Sartorius Stedim Biotech MD8 airscan, Руководство по эксплуатации, Страница: 5 / 57

1. Introduction
The MD8 airscan is an air sampler which works according to the filtration principle. It draws
a defined volume of air through a gelatin filter or equivalent. The microorganisms and viruses
contained in the sampled air are retained by the filter, where they are available for processing
by microbiological or virological methods.
When the microorganism content of the sampled air is to be quantitatively determined, the
usual method is to subsequently plate the filter directly on an appropriate culture medium,
incubate and count the resulting number of colony forming units (cfu). This number is related
to the volume sampled to give cfu/m
3
.
When the virus concentration is to be determined, the gelatin filter must be dissolved after
sampling, so that retained viruses can be cultivated in the solution and analysed by virological
or molecular biological methods. Detailed procedural information is given in the application
notes “Collecting Airborne Viruses and Phages Using the Gelatin Membrane Filter Method”
and “A method for detecting and enumerating airborne virulent bacteriophage of dairy starter
cultures” (publication numbers SLF-4028-e and SM-4021e respectively) as well as in further
publications on this topic.
Further information on request.
| 5