18
Galaxy S+ CO
2
Incubator
User Guide
#
NOTE:
CO
2
will not come on until the programmed temperature has been reached.
Ensure gas supply connections are secure: gas leaks not only affect
performance, they will also deplete the gas supply.
7.
The chamber set points are pre-programmed at 37.0°C and 0.0% CO
2
.
8.
If you are not planning to humidify the incubator, leave the humidity tray in place
and
skip to Step 4 in Section 3.3 below
.
3.3
Using the Humidity Tray
1.
Fill the humidity tray with 1.5 litres of warm (
∼
37.0°C) distilled water.
2.
For cell culture work
, we recommend the addition of one small teaspoonful (3.2g)
of copper sulphate (or another recognised biocide) in the humidity tray. Tests
have shown that in addition to inhibiting bacterial growth in the tray, this can
reduce contamination on the chamber walls.
3.
For IVF and other sensitive work
, we do not recommend the use of any biocide
in the humidity tray. To reduce the possibility of contamination, every 10 - 14
days the humidity tray water should be changed and the humidity tray should be
cleaned with a solution of 70% isopropyl alcohol and 30% distilled water.
CAUTION!
Never leave water in the humidity tray if the incubator is switched OFF,
as this may cause damage to the CO
2
sensor.
4.
Let the incubator run until programmed conditions are achieved (preferably
overnight), to allow conditions to stabilise within the incubator before use.
3.4
Setting Temperature & CO
2
At this time, program the desired temperature and CO
2
set points. Temperature and
CO
2
may be set within the following ranges (
see Table 4 on the following page
):
