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Example: 

Calculating Irradiation Time If Dose is NOT listed on Dose Chart 

Suppose you wish to give a specimen a dose of 34 Gy on Level 3. Looking at the Dose Chart, 
the dose rate at Level 3 is 1.8 Gy/minute. Dividing dose by the dose rate                                   
(34 ÷ 1.8) gives a total irradiation time of 18.89 minutes. 

 

 

  Doses are for machine setting: 160 KV, 25mA   

 

 

 

RAD+(blac

k  boran 

carbonite 

box)

 

 

 

ANIMAL 

CAGE

 

 
 

 

LEVEL 1

 

 
 

 

LEVEL 2

 

 
 

 

LEVEL 3

 

 
 

 

LEVEL 4

 

 
 

 

LEVEL 5

 

 

 

cGy/Rad 

dose

 

 

 

Gray(Gy) 

dose

 

Dose Rate:

 

112 rads 

Or 

1.1Gy/min

 

Dose Rate:

 

114 rads 

Or 

1.4Gy/min

 

Dose Rate:

 

101 rads 

Or 

1.0Gy/min

 

Dose Rate:

 

132 rads 

Or 

1.3Gy/min

 

Dose Rate:

 

180 rads 

Or 

1.8 Gy/min

 

Dose Rate:

 

256 rads 

Or 

2.6 Gy/min

 

Dose Rate:

 

410 rads 

Or 

4.1 Gy/min

 

100 

54S 

55S 

1M 00S 

46S 

34S 

22S 

15S 

250 

2.5 

2M 14S 

2M 11S 

2M 28S 

1M 53S 

1M 23S 

59S 

33S 

500 

4M 28S 

4M 23S 

4M 57S 

3M 47S 

2M 47S 

1M 57S 

1M 13S 

1,000 

10 

8M 56S 

8M 47S 

9M 54S 

7M 35S 

5M 33S 

3M 55S 

2M 27S 

1,500 

15 

13M 25S 

13M 09S 

14M 51S 

11M 22S 

8M 19S 

5M 51S 

3M 39S 

2,000 

20 

17M 51S 

17M 33S 

19M48S 

15M 9S 

11M 06S 

7M 49S 

4M 53S 

2,500 

25 

22M 19S 

21M 56S 

24M 45S 

18M 57S 

13M 53S 

9M 46S 

6M 06S 

3,000 

30 

26M 48S 

26M 19S 

29M 42S 

22M 43S 

16M 40S 

11M 43S 

7M 19S 

3,500 

35 

31M 15S 

30M 42S 

34M 39S 

26M 32S 

19M 27S 

13M 41S 

8M 33S 

4,000 

40 

35M 42S 

35M 06S 

39M 36S 

30M 18S 

22M 14S 

15M 38S 

9M 45S 

6,000 

60 

53M 35S 

52M 32S 

59M 24S 

45M 27S 

33M 19S 

23M 27S 

14M 37S 

10,000 

100 

see Deb 

see Deb 

see Deb 

see Deb 

55M 34S 

39M 04S 

24M 24S 

12,500 

125 

see Deb 

see Deb 

see Deb 

see Deb 

1H 09M 27S  48M 51S 

30M 29S 

17,500 

175 

see Deb 

see Deb 

see Deb 

see Deb 

see Deb  1H 08M 22S  42M 41S 

20,000 

200 

see Deb 

see Deb 

see Deb 

see Deb 

see Deb 

see Deb 

48M 47S 

 

   1cGy=1 Rad 
    1Gy=100cGy (100 Rads)

 

 

  10Gy=1000cGy (1000 Rads)=1 kiloRad   

 

 

 

 

100Gy=10000cGy 
 

Note that some of the times have a notation to “See Deb” that is because if the 
machine is continuously ran for more than an hour it will over heat and shut down.  
To prevent the machine from overheating we run our sample for 59minutes, let the 
machine turn off the X-ray but keep the fans going for 15 minutes, this allows the 
200 gallons of internal water to cool down to maintain a lower core temp for the X-
ray tube.  Once the cool down is done, simply enter your remaining time and 
continue your run. 
 
In most cases, the Dose Chart will provide the irradiation time needed. However, 
if a dose is not provided you can use the dose rate to calculate the irradiation 
time, as shown in the example below:

 

 
 
 
 
 
 
 

 

 

Содержание RS-2000

Страница 1: ...egulatory Dose Limit General Hazards Associated with the X Ray Irradiator Safety Instructions Regarding the RS 2000 Irradiator Operating Instructions for the RS 2000 Irradiator 2 More complete and det...

Страница 2: ...ct with the body So our next question is What is ionization and why is it a concern All matter is made up of atoms Atoms contain a small central nucleus containing protons which have electrically posi...

Страница 3: ...called an ion The process of ionization is illustrated below This freed electron can potentially cause damage to living cells or DNA The damage can be repaired from small amounts of radiation however...

Страница 4: ...generate x rays see picture below Basically what happens is that a metal filament is heated up to a very high temperature allowing electrons from the metal to essentially boil off Electrons the small...

Страница 5: ...ill I receive from handling irradiated specimens and from using this irradiator As for handling irradiated specimens they do NOT become radioactive after an irradiation and you will not receive any ra...

Страница 6: ...s No unauthorized personnel may defeat or override any safety features on the x ray generator collimator or shielding without permission of the manufacturer Rad Source The RS 2000 is to be serviced by...

Страница 7: ...Indication Lights show when X rays are being produced Shielded and Interlocked X Ray Tube Chamber to prevent X ray production if panel should be opened Door Handle Double Safety Interlocked to prevent...

Страница 8: ...ning module must be documented in order to get Xray access This training is different that the Radiation training orientation you need to complete both Radiation Safety for Radioactive Material Users...

Страница 9: ...t Here are the different steps that may need to be taken Let me know which one fits your needs and I will send you the correct forms 1 If you currently have a mouse protocol and have mice housed in CC...

Страница 10: ...gaged unit will not operate if not properly closed This can be done after you load your samples animals and set your time 2 Use the key to turn the power on off switch to on the key to power on the un...

Страница 11: ...d that over heating can cause the unit to shut down I also have an electric fan in the room that you can set up to bring cooler air from the hall into the room 5 Main Menu The main menu enables the op...

Страница 12: ...re two pre programmed warm up cycles labeled NORMAL and EXTENDED Unless you know that the unit hasn t been used for a couple of months select NORMALNORMAL 4 Press START at the bottom of the screen 5 T...

Страница 13: ...re typically irradiated on Level 1 i e within the black RAD reflective chamber on the floor of the chamber Place no more than 5 mice in a CLEAN cage Remove food and water from the cage if you have exc...

Страница 14: ...ormity is 98 The radiation dose rate is highly dependent upon the distance from the radiation source x ray tube Therefore for certain specimens e g test tubes flasks plates you should lay items horizo...

Страница 15: ...S 29M 42S 22M 43S 16M 40S 11M 43S 7M 19S 3 500 35 31M 15S 30M 42S 34M 39S 26M 32S 19M 27S 13M 41S 8M 33S 4 000 40 35M 42S 35M 06S 39M 36S 30M 18S 22M 14S 15M 38S 9M 45S 6 000 60 53M 35S 52M 32S 59M 24...

Страница 16: ...an irradiation time in for one programs A B C or D If you have already programmed in an irradiation time go to Step 3 After selecting PROGRAM SETUP the following screen AUTO PROGRAM SETUP will appear...

Страница 17: ...afety Cabinet If the BSC is used spray and wipe down all surfaces inside the BSC Repeat with TEC Surf solution c Please dispose of all debris and waste in the waste basket provided d Empty the bleach...

Страница 18: ...rrective actions for the RS 2000 Most of the time you will just need to hit clear and continue on If that doesn t work turn off the machine wait 20 seconds and turn it back on If neither of those opti...

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