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QIAamplifier 96 User Manual 03/2020

Creating, editing, and saving programs

8.1

Programming modes

The QIAamplifier 96 software offers spreadsheet or graphical programming. Use the buttons to
toggle between the two modes. The following screenshots show the spreadsheet and graphical
programming mode.

Graphical view

Spreadsheet view

Figure 14. Spreadsheet programming.

Содержание 1120412

Страница 1: ...Sample to Insight__ March 2020 QIAamplifier 96 User Manual For Molecular Biology Application Not for use in diagnostic procedures QIAGEN GmbH QIAGEN Strasse 1 D 40724 Hilden 1120412...

Страница 2: ...l 11 2 5 Safety instructions transport and installation 12 2 6 Safety instructions during operation 12 2 7 Behavior during emergencies 14 3 Technical Specifications 15 4 Function and setup 16 4 1 High...

Страница 3: ...g editing and saving programs 46 8 1 Programming modes 46 8 2 Creating a new program using a program template 48 8 3 Assigning program names 50 8 4 Setting the heated lid temperature 50 8 5 Preheating...

Страница 4: ...hooting 107 13 1 Administrator password forgotten 107 13 2 Slow heating and cooling 107 13 3 Auto restart 107 13 4 Auto restart without an apparent cause 107 13 5 Adaptation of programs from other the...

Страница 5: ...QIAamplifier 96 User Manual 03 2020 5 Waste Electrical and Electronic Equipment WEEE 116 Index 118 Document Revision History 120...

Страница 6: ...theoretical expertise in molecular biology and the use of QIAGEN products If you have any questions or experience any difficulties regarding QIAamplifier 96 or QIAGEN products in general please do no...

Страница 7: ...n 1 3 Intended use of QIAamplifier 96 The QIAamplifier 96 is an end point thermal cycler It is designed to amplify nucleic acids by repeated cycles of heating and cooling using DNA polymerases in a PC...

Страница 8: ...r several of the following causes Use of the QIAamplifier 96 other than intended Improper commissioning operation and servicing of the device Modifications of the QIAamplifier 96 without prior consult...

Страница 9: ...References to potential dangers do not replace the work protection regulations which must be observed 2 1 Symbols and signal words used This manual uses the following symbols and signal words to indi...

Страница 10: ...actions leading to personal injury or material damage Important Do not remove safety symbols Replace damaged safety symbols immediately The following safety symbols are attached to the inside of the...

Страница 11: ...ion The technical condition must always comply with the legal requirements and regulations Prior to every use the device must be checked for damage and sound condition Any changes in the device affect...

Страница 12: ...ach commissioning that the condition of the device including the safety equipment is sound Observe the following Free access to the power switch on the back of the enclosure has to be ensured during o...

Страница 13: ...by the customer service of QIAGEN and specially authorized technicians The electrical components must be checked regularly by a qualified electrician Any defects such as loose connections and faulty...

Страница 14: ...ial 2 6 5 Safety instructions service and repair The QIAamplifier 96 is usually repaired by the service department of QIAGEN or its authorized and trained specialist personnel Unauthorized repairs can...

Страница 15: ...e info file SINF generation expanded self test adjustable ramp rates gradient temperature diagram view Program memory Total capacity of 350 programs in up to 90 user directories Language English Displ...

Страница 16: ...ns on a PC Figure 1 The QIAamplifier 96 Thermal Cycler The QIAamplifier 96 thermal cycler features an automatic restart If a power failure occurs during the run the device will continue the run as soo...

Страница 17: ...ve the block surface level and it ensures reliable contact between the reaction tubes and the thermoblock by applying constant pressure Thanks to the integrated clutch mechanism the pressure applied t...

Страница 18: ...f time if for each single step all parameters have to be set manually The QIAamplifier 96 software offers several pre installed program templates for different applications The program templates provi...

Страница 19: ...ubes strips or plates PCR plates can be standard profile or low profile and they can be skirted half skirted or non skirted The QIAamplifier 96 can handle all kinds of plates and the plasticware can b...

Страница 20: ...ttempt to operate the device if there is any visible damage Keep the original packaging for possible return The QIAamplifier 96 must always be transported in its original packaging If the packaging is...

Страница 21: ...QIAamplifier 96 User Manual 03 2020 21 Figure 1 Voltage selector switch on the underside of the device...

Страница 22: ...tion Place the device at a minimum distance of 10 cm between the rear and sides of the device and the wall or other objects Place the QIAamplifier 96 on a solid dry surface If the QIAamplifier 96 has...

Страница 23: ...r 96 User Manual 03 2020 23 6 Control elements 6 1 QIAamplifier 96 front view 1 Dial for adjusting the contact pressure 2 Lid sealing button 3 Display 4 USB port Figure 2 Front view of the QIAGEN QIAa...

Страница 24: ...6 2 QIAamplifier 96 rear view The Ethernet port mains plug and mains switch are located at the rear of the QIAGEN QIAamplifier 96 1 Ethernet port 2 Mains switch 3 Fuse holder 4 Mains plug Figure 3 Re...

Страница 25: ...e until the clutch mechanism is activated you will hear a clicking noise ATTENTION The strength of the lid contact pressure has been designed for a fully populated block If only a few samples are to b...

Страница 26: ...elow when opening the lid If the lid is opened under pressure the locking mechanism may be damaged 1 Reduce the contact pressure of the lid completely by turning the lid wheel counterclockwise until t...

Страница 27: ...ation 6 4 1 Keyboard For some applications names addresses or passwords have to be defined For this purpose the QIAamplifier 96 software offers a keyboard to enter characters digits and special charac...

Страница 28: ...maximum of 3 characters 6 4 2 Keypad In some screens of the QIAamplifier 96 software digits have to be entered For this purpose the software features a keypad integrated in some screens Figure 6 QIAa...

Страница 29: ...igher than the maximum number that can be displayed on a screen the software will show cursor keys for navigation Scroll up Scroll right Scroll down Scroll left 6 4 4 Confirm or cancel On many screens...

Страница 30: ...30 6 4 5 List of commands and icons used The QIAamplifier 96 software uses the following icons Command Icon Home Back Right Left Up Down Confirm Cancel Login User management New user Delete user Edit...

Страница 31: ...rom previous page Command Icon Incubation Start incubation Programs New program Open template Copy program copy all programs Delete program Edit program Start program Quick select program or user Stop...

Страница 32: ...inued from previous page Command Icon Pause program Continue program Skip step Graphical view Spreadsheet view Gradient display Edit step Insert step Delete step Time increment Heating rate Gradient L...

Страница 33: ...m previous page Command Icon Standard gradient programming Preheating OFF Preheating ON Tools Settings Date and time Automatic user logout Beeper Network Display brightness Display darker Display brig...

Страница 34: ...n Factory settings Documentation Run log file Run log file overview Save run log file to USB Save all run log files to USB View run log file View program Log file self test Error log file Extended sel...

Страница 35: ...QIAamplifier 96 User Manual 03 2020 35 Table continued from previous page Command Icon Backup file Save backup file Load backup file Cycler information...

Страница 36: ...power on self test the login screen is displayed The login screen offers the following functions Command Function Chapter Login Starts the login dialog See chapter 7 3 Block Shows the current block s...

Страница 37: ...the most recently logged in user is always shown in the first position at the top left of the screen All the other buttons become arranged from top left to bottom right according to the date and time...

Страница 38: ...QIAamplifier 96 User Manual 03 2020 38 Figure 9 QIAamplifier 96 enter password screen...

Страница 39: ...grams Viewing running and editing programs See chapter 8 Block Shows the current block status If a block is free a touch on the button leads to a list of previously used or changed programs for quick...

Страница 40: ...haracters are shown in red and the warning Hot plus the corresponding warning symbol are displayed In the line below the textbox the remaining runtime is shown The sample block number is shown above t...

Страница 41: ...QIAamplifier 96 User Manual 03 2020 41 Button Block status Color Free Green Running Yellow Pause Blue...

Страница 42: ...e a touch of the button will lead to the program view screen instead If the block status button is used in the login screen before a user is logged in a list of the programs most recently used on the...

Страница 43: ...tart to start the selected program If necessary the program can be edited before the start or a new program can be created based on a template see chapter 8 1 Start Press the corresponding button to a...

Страница 44: ...ss Incubate and in the next screen set the values for the block temperature and hold time using the keypad Set whether the heated lid should be on or off and whether it should preheat or not using the...

Страница 45: ...ds can be used Stop Pause Continue Skip Note To stop the incubation use the corresponding command Do not switch off the device while the incubation is running The device will react like after a power...

Страница 46: ...Programming modes The QIAamplifier 96 software offers spreadsheet or graphical programming Use the buttons to toggle between the two modes The following screenshots show the spreadsheet and graphical...

Страница 47: ...ode Chapter Program name See chapter 8 3 Heated lid temperature C See chapter 8 4 Preheat lid mode See chapter 8 5 Step number See chapter 8 6 Temperature C See chapter 8 6 Hold time h mm ss See chapt...

Страница 48: ...1 2 After login press Program in the home screen see chapter 7 3 Program The program overview screen opens Figure 16 QIAamplifier 96 program overview screen 3 In the program overview screen press New...

Страница 49: ...lifier 96 Blank program template Select Blank whenever a new program should be created and select another template from the list whenever an existing program should be edited The program structure can...

Страница 50: ...6 4 1 Note If a character is pressed the pre displayed name will be deleted and a new program name can be set If the program name should be modified first press another button like delete or space 8...

Страница 51: ...cessary cooling against the heating cover is required 8 5 Preheating the heated lid The heated lid can be set to preheating ON or OFF using the corresponding buttons Preheat heated lid Off Preheat hea...

Страница 52: ...ch the target temperature is increased or decreased with each cycle The temperature increment can be 20 0 C See chapter 8 6 1 2 Time increment e g for long range PCR by which the time is increased wit...

Страница 53: ...n Figure 20 QIAamplifier 96 program step editing screen Note To program a step at least the temperature and the hold time must be defined To set the temperature touch the corresponding input field and...

Страница 54: ...g of loops For a loop two parameters have to be set 1 The step number to which the program should return Go To 2 The number of repetitions Cycles To program a loop select the last step of the loop and...

Страница 55: ...n the graphical programming mode 8 6 1 3 Programming a time increment For some applications for example long range PCR time increments are used The time is increased in each cycle by the set value To...

Страница 56: ...8 6 1 5 Programming a gradient step The gradient function is most often used to optimize new primer pairs A temperature gradient is established across the sample block in the annealing step so that t...

Страница 57: ...sample block The effective temperatures for all the other columns are automatically calculated and displayed as a bar chart see Figure 23 Note The annealing temperature is set for a specific column i...

Страница 58: ...sing the standard gradient programming screen enter a temperature for the first column and the last column in the sample block see Figure 24 The effective temperatures for all the other columns are au...

Страница 59: ...l or spreadsheet view To edit a parameter for a step press directly on the cell for the program parameter in the table see Figure 25 Figure 24 QIAamplifier 96 spreadsheet program view Parameter Spread...

Страница 60: ...mplifier 96 graphical program view Parameter Spreadsheet view Temperature gradient C input field Hold time h mm ss input field Loop counter option button Temperature increment option button Time incre...

Страница 61: ...6 User Manual 03 2020 61 Figure 26 QIAamplifier 96 individual program parameter editing You also have the option of programming a gradient step To do this when editing the temperature press the Grad b...

Страница 62: ...iting program steps opens see chapter 8 6 Enter the desired parameters for the new step Note The new step will be inserted at the selected program step If for example step 3 is selected the new step w...

Страница 63: ...h the corresponding buttons to select a user directory and a program storage location If the number of user directories or programs is higher than the maximum number that can be displayed in the list...

Страница 64: ...or a program location using the quick select function Touch the icon above the user or program view see Figure 29 Quick select function An overview screen opens in which a maximum of 30 users or 50 us...

Страница 65: ...29 QIAamplifier 96 quick select screen Before saving the program there is still the possibility of changing the program name To do this press Save as and enter the new program name using the QIAamplif...

Страница 66: ...a program to be started press Programs on the QIAamplifier 96 home screen see chapter 7 4 Programs The program overview screen opens with the directory of the logged in user preselected see Figure 31...

Страница 67: ...ram before it is started To start the selected program press Start Start Figure 31 QIAamplifier 96 program overview screen with preview 9 2 Copying a program To copy a program touch the corresponding...

Страница 68: ...ready in use the stored program will not be overwritten instead this program and all other following programs will be moved one position higher The storage location number for these programs will incr...

Страница 69: ...Save Save Note The programs will be copied into the selected user directory beginning at the first free storage location Figure 33 QIAamplifier 96 program overview screen 9 4 Deleting a program To de...

Страница 70: ...e by 1 9 5 Deleting all programs To delete all programs touch the corresponding buttons to select a user directory on the QIAamplifier 96 program overview screen see Figure 31 If the number of user di...

Страница 71: ...s been started see chapter 9 1 the QIAamplifier 96 software can display the currently running program in a spreadsheet or graphical view In addition the curve of the gradient can be displayed in gradi...

Страница 72: ...ifier 96 display during the run in a graphical spreadsheet and gradient view The following parameters are summarized in the headline in the table Program name Sample block temperature Estimated end He...

Страница 73: ...the graphical view each step is divided into a heating phase and a hold time and both parts are highlighted separately Time and temperature increments and ramp rate adjustments cannot be shown in the...

Страница 74: ...ime field Spreadsheet view Graphical view Figure 37 QIAamplifier 96 run display screen during a program pause Note Pressing the Pause button requires manual interaction The QIAamplifier 96 software al...

Страница 75: ...am To continue a paused program press Continue on the QIAamplifier 96 run display screen see Figure 38 Continue The QIAamplifier 96 will continue the program from exactly the point at which it was pau...

Страница 76: ...QIAamplifier 96 User Manual 03 2020 76 Figure 38 QIAamplifier 96 confirmation prompt After confirmation of the confirmation prompt the QIAamplifier 96 stops the current program...

Страница 77: ...mentation Access to stored log files See chapter 11 3 Self Test Executes the extended self test See chapter 11 4 Service info file Creates service info files SINF See chapter 11 5 Backup Saves all fol...

Страница 78: ...ork Defines network settings See chapter 11 1 4 Display brightness Adjusts the display brightness See chapter 11 1 5 Touch screen calibration Calibrates the touchscreen See chapter 11 1 6 Factory sett...

Страница 79: ...ield and use the keypad on the right side of the date and time entry screen to enter digits The following buttons may also be helpful when completing the fields Delete single character Delete all char...

Страница 80: ...button Automatic logout On Automatic logout Off If automatic user logout is switched on the time after which the user is logged out from the system can be set You can enter values between 30 and 60 mi...

Страница 81: ...r configuration screen 11 1 4 Network The Network menu item can be used to configure basic settings or show a list of network users Press the corresponding button Network settings Network user 8 6 1 6...

Страница 82: ...Delete all characters Go to the next input field Figure 44 QIAamplifier 96 network settings screen Note The network cable must be at least performance class Cat 5e and the cable configuration must be...

Страница 83: ...slider Darker Brighter Figure 45 QIAamplifier 96 display brightness adjustment screen 11 1 6 Screen calibration To calibrate the screen follow the instructions and touch the screen at the center of th...

Страница 84: ...prompt Figure 47 QIAamplifier 96 confirmation prompt Using the factory settings tool will delete all users and programs Use a backup file to restore the system see chapter 11 6 11 2 User management T...

Страница 85: ...s Figure 48 QIAamplifier 96 user management screen Available users are listed on the QIAamplifier 96 user management screen see Figure 49 sorted by the date and time of the last login and the username...

Страница 86: ...he QIAamplifier 96 keyboard see chapter 6 4 1 Username up to 13 characters or numbers Initials 2 to 3 characters Figure 49 QIAamplifier 96 create new user screen 11 2 2 Editing user settings To edit t...

Страница 87: ...e first system start and to create a backup of the system Folders programs users and rights settings can be restored from a backup file 11 2 3 Delete user To delete a user select the user on the user...

Страница 88: ...the following documentation tools Run Log File Documentation of PCR runs See chapter 11 3 1 Power On Log File Result of the latest initial self test See chapter 11 3 2 Self Test Log File Result of th...

Страница 89: ...d is shown at the first position in the table The initials of the user who started the program the number of the sample block being used the program name the directory and the program number are also...

Страница 90: ...rsion User initials Cycler type Serial number of the QIAamplifier 96 thermal cycler Sample block type Block serial number Block number Messages Figure 54 QIAamplifier 96 run log file screen To view th...

Страница 91: ...en the run log file screen and the view program screen Overview Run Log Files View Run Log File Show Program To export all the saved run log files on USB press Save all Run Log Files on USB To export...

Страница 92: ...and time Last power down by date and time A table with a list of error messages by number date time error code and message Figure 56 QIAamplifier 96 power on log file screen 11 3 3 Extended self test...

Страница 93: ...n be Passed or Failed and is listed in the last row of the table for each test 11 3 4 Error log file The QIAamplifier 96 records all errors by date and time The latest error is shown at the first posi...

Страница 94: ...at sink and the fans are working properly together Gradient Checks if the sample block reaches the set gradient temperatures Heated lid Tests if the heated lid reaches the set temperature and can hold...

Страница 95: ...tended self test can be started To start the extended self test press Start Self Test Figure 60 QIAamplifier 96 extended self test screen The software provides a progress overview during the extended...

Страница 96: ...mplifier 96 extended self test overview screen The results of the extended self test are summarized in a log file stored by the device and can be viewed by the user see chapter 11 3 3 11 4 Service inf...

Страница 97: ...stored to USB and transferred to another QIAamplifier 96 Figure 62 QIAamplifier 96 backup file screen Connect a USB stick to the device and press Save backup file to create a backup file Save backup...

Страница 98: ...ock type Block serial number Software version Protocol version Revision number power board Software version power board Software version power board logic Revision number processor board Software vers...

Страница 99: ...QIAamplifier 96 User Manual 03 2020 99 Figure 65 QIAamplifier 96 Cycler Info Screen...

Страница 100: ...tailed description of the error situation This information will help you and your QIAGEN Technical Service Specialist to deal most efficiently with your issue For technical assistance and more informa...

Страница 101: ...and may come to an explosive boil Wear goggles when handling hot samples Make sure that the lid is closed securely before starting the program NOTICE The use of oil between samples and sample block is...

Страница 102: ...Tools to access the system settings user management documentation backup tool or self test functions Press Logout to log out Editing saving and copying programs Press Programs on the QIAamplifier 96...

Страница 103: ...id preheating on or off press the corresponding button Select a program step and press Edit The screen for editing program steps opens Use the cursor keys above the keyboard to switch between program...

Страница 104: ...sheet or graphical program display Alternatively individual program parameters for a step can also be edited To do this in the spreadsheet view press directly on the cell for the parameter in the prog...

Страница 105: ...n prompt Starting stopping and pausing programs The QIAamplifier 96 stores the latest 5 started or edited programs for each user To quick start a program press Quick Start on the QIAamplifier 96 home...

Страница 106: ...aused program press Continue To skip a step press Skip The program will continue with the next step To stop an active program press Stop program and confirm the confirmation prompt Programs with a pau...

Страница 107: ...o restart function If a power failure occurs during a run the device will continue the run at the same point when the power returns In case of long term power failure longer than 30 minutes the device...

Страница 108: ...this situation the clutch mechanism is active in both directions there is a clicking noise in both directions To unlock the lid wheel press down the metal pin on the top of the lid wheel with a pen Th...

Страница 109: ...vice department immediately In order to guarantee sound and safe operation and to ensure laboratory certification we recommend concluding a maintenance agreement with regular device validation 14 1 Cl...

Страница 110: ...ing spray disinfectants there is a risk that the liquid enters the sensitive electronic system through the ventilation slits If the disinfectant has a spray nozzle apply disinfectant to a suitable clo...

Страница 111: ...y damage caused by inadequate decontamination of the device Please clean all device components from biologically hazardous chemical or radioactive contamination see also section Disinfecting the devic...

Страница 112: ...s an extra designed packaging system with inserts of foamed material Important The thermal cycler is only protected from transport damage if the original packaging is used and the packing instructions...

Страница 113: ...dispose of any waste materials that occur during measurements sample materials in accordance with statutory and local regulations At the end of its service life the QIAamplifier 96 and all its electr...

Страница 114: ...3 2020 114 Appendix A Declaration of Conformity Name and address of the legal manufacturer QIAGEN GmbH QIAGEN Strasse 1 40724 Hilden Germany An up to date declaration of conformity can be requested fr...

Страница 115: ...QIAamplifier 96 User Manual 03 2020 115 Certificates For up to date certificates you may request them from QIAGEN Technical Services or from your local QIAGEN representative...

Страница 116: ...ures that the product is recycled in a manner that protects human health and the environment Recycling can be provided by QIAGEN upon request at additional cost In the European Union in accordance wit...

Страница 117: ...duration of the original warranty period and in no case beyond the original expiration date of original warranty unless authorized in writing by an officer of the Company Read out devices interfacing...

Страница 118: ...cubation 39 Liability 7 Login 33 Login screen 33 Maintenance 106 Manual Nomenclature 7 Operating voltage 20 Packaging 109 Password Administrator 34 Forgotten 104 Personnel 11 Power on self test 32 Pro...

Страница 119: ...Display brightness 78 Factory settings 79 Network 76 Screen calibration 78 User management 79 Signal words 9 Step Skip 70 Symbols Manual 9 Tools 72 Contact 96 Cycler Info 95 Extended self test 90 Ser...

Страница 120: ...QIAamplifier 96 User Manual 03 2020 120 Document Revision History Date Changes March 2020 Initial release...

Страница 121: ...arties 2 Other than expressly stated licenses QIAGEN makes no warranty that this kit and or its use s do not infringe the rights of third parties 3 This kit and its components are licensed for one tim...

Страница 122: ...QIAamplifier 96 User Manual 03 2020 122 1120412 HB 2739 001 03 2020 Ordering www qiagen com shop Technical Support support qiagen com Website www qiagen com...

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