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QIAamplifier 96 User Manual 03/2020 18
4.4 User-specific quick start of programs
Many other thermal cyclers offer a so-called “List of latest programs used”. In the list of latest
programs used, a certain number of PCR programs is collected and offered for quick start. The list
is typically ordered only by date and the user has to search for the desired program. In contrast,
the QIAamplifier 96 software creates a user-specific list and only the latest programs started by the
currently logged-in user are offered for quick start. Even if a lab member does not use the device
for a longer time, the information on the latest programs started by this user will not get lost.
For maximum convenience, the QIAamplifier 96 software additionally features a program preview.
Before the start of a program the protocol steps are summarized in a clearly arranged table by the
program preview tool. The preview therefore provides a comprehensive overview of the protocol
structure without the need to access programming screens. The program preview is also available
before the quick start of programs.
4.5 Easy programming
Creating new PCR programs takes a lot of time if for each single step all parameters have to be set
manually. The QIAamplifier 96 software offers several pre-installed program templates for different
applications. The program templates provide a general protocol structure that can be easily
adapted for the current experiment.
4.6 Linear gradient tool
For optimizing new primer pairs in a single run, the QIAamplifier 96 is equipped with a gradient
function. Finding the best primer annealing temperature is crucial for the specificity and efficiency
of PCR reactions. Often the optimization of experiments is conducted to a limited extent only,
leading to unspecific byproducts or reduced PCR sensitivity. By using the gradient function, new
primer pairs with unknown annealing temperatures can be tested quickly and optimized in a very
short time.
For maximum convenience, the QIAamplifier 96 offers the Linear Gradient Tool. For most other
devices, the temperature difference from row to row or from column to column in a gradient step
varies across the sample block. The QIAamplifier 96 Linear Gradient Tool enables the programming
of gradient steps with a defined temperature increment between the columns or rows. The Linear
Gradient Tool option enables the comfortable creation of gradient steps and thus the best annealing
temperature can be easily transformed to a non-gradient protocol.