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10
Retiga 3000 USER MANUAL
ROI / Binning
The Retiga 3000 supports both user-defined, arbitrary regions of interest (ROI) as well as hardware pixel
binning modes of 1x1, 2x2, 4x4, and 8x8. Both ROIs and pixel binning will enable faster frame rates, making
focusing and scanning much easier. Pixel binning also has the advantage of significantly increasing the signal-
to-noise ratio (SNR) of the image.
For color cameras, pixel binning will result in the loss of the native color interpolation. When using the Retiga
3000 color camera for low light fluorescence documentation, binning can be used to improve SNR, but
pseudo-coloring will be required to obtain color images. Pseudo-coloring is a commonly used method and is
a function typically available in most imaging software applications (including QCapture Pro 7). This will allow
you to apply the tint of the fluorescent labels used to the acquired images for documentation and publication
purposes. When using pixel binning, the acquired images will appear to be grayscale although they will have
an RGB pixel value. Simply convert the images to a single channel greyscale value (8-bit mono, 12-bit mono,
etc.) and use your software application to apply a color tint.
Device Synchronization
Your Retiga 3000 camera offers several methods of hardware synchronization via TTL signals with external
devices, including function generators, light sources, shutters and filters. Each camera has an I/O connector (pin
out functions are described in the specifications chapter) on the back for trigger-in/out and various TTL input
and output operations.
A special cable is available to access primary signals such as “Trigger-in,” “Expose out,” and “Frame Readout”.
In the default mode, the camera triggers on the rising edge of a TTL signal. Retiga 3000 cameras support three
trigger modes:
Trigger-first mode
In this mode, the camera requires one trigger to begin the acquisition of a stream of images. Once a single
trigger is received, the camera will use its internal clock to acquire the entire image stream, independent of
any future triggers. It is possible to run this triggering mode in either Non-Overlap mode (left) or Overlap mode
(right).
Содержание Retiga 3000
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