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TM248 · Revised 12/18
10. Repping, S.
et al. (
2002) Recombination between palindromes P5 and P1 on the human Y chromosome causes
massive deletions and spermatogenic failure.
Am. J. Hum. Genet
.
71
, 906–22.
11. Saxena, R.
et al.
(1996) The DAZ gene cluster on the human Y chromosome arose from an autosomal gene that
was transposed, repeatedly amplified and pruned.
Nat. Genet.
14
, 292–9.
7. Appendix
7.A. Composition of Buffers and Solutions
1X TBE buffer
89mM Tris-base
110mM boric acid
2mM EDTA
7.B. Related Products
Accessory Components
Product
Size Cat.#
Nuclease-Free Water
(2 × 25ml) 50ml
P1193
Mineral Oil
12ml
DY1151
DNA Purification Systems
Product
Size Cat.#
Wizard
®
Genomic DNA Purification Kit
100 isolations × 300µl
A1120
500 isolations × 300µl
A1125
100 isolations × 10ml
A1620
MagneSil
®
KF, Genomic System
200 preps
MD1460
7.C. Reaction Setup for Small Sample Numbers
This protocol is designed to analyze a small number of samples. The order of addition of
Taq
DNA polymerase,
Multiplex Master Mixes and genomic DNA is different from the Large Sample Number Protocol (Section 4.A). These
changes conserve the Multiplex Master Mixes to ensure the full number of reactions can be performed.
In this procedure:
•
The Multiplex Master Mixes are added to the reaction tubes.
•
A mixture of genomic DNA and Taq DNA polymerase in Nuclease-Free Water is prepared.
•
The genomic DNA/Taq DNA polymerase mixture is added to each Multiplex Master Mix.
•
Each sample DNA is analyzed with all five of the Multiplex Master Mixes.
