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PriorLux
™
7
G. Oil Immersion Objectives Only
For oil immersion lenses, make sure that a part of the specimen is in the centre of the
field of view as seen with the last dry objective used. Rack the nosepiece up slightly,
swing the dry lens to one side, rotate the 100x oil immersion lens over the specimen
ensuring that it clears the cover-slip. If there is insufficient clearance, rack the
nosepiece up further. Lower the lens close to the slide and then rotate it slightly to
one side. Place a drop of immersion oil (free of air bubbles) on the specimen and
gently rotate the lens into the drop. Using the fine focus knob, focus the image.
Proceed as before through steps B to F. Finally, set the focus stop to ensure that the
front lens of the objective does not touch the slide.
Remove residual oil from the objective after use.
8. Cleaning Objectives
It is critical that the front lens of each objective is kept clean and free of contamination.
Any dust or dried immersion oil will seriously affect the image quality attainable with that
objective. If contamination is suspected then the easiest way to confirm this is by removing
the objective lens and examining the front lens using the eyepiece. To do this take out one
of the eyepieces, turn it around so that you are looking the wrong way through it and move
it towards the front of the objective until you can focus on the front lens. This will clearly
show any contamination. To remove dirt and oil a lens cloth, lens tissue or cotton bud
dampened with industrial alcohol can be used. A spiral motion starting from the centre of
the lens moving to the outside is the best way of achieving a thoroughly clean surface