Vetlab Supplies Ltd Unit 13 Broomers Hill Park Broomers Hill Lane Pulborough RH20 2RY
Telephone 01798 874567 Fax 01798 874787 Email
www.vetlabsupplies.co.uk
Begin observation of your specimen with the 4x Objective. Turn the coarse
focusing knob until a clear image is obtained, then use the fine focus knob to
enhance the observation of the specimen to its clearest image. On the coarse
focus knob is a tension control ring (located on the right side of the microscope
against the stand) which can be turned to tighten or loosen the tension of the
focusing knob to the user’s desired setting.
When the desired view is obtained under the lowest power (4x), rotate the
nosepiece to the next higher magnification (10x). The nosepiece should “click”
into position. It should only be necessary to adjust the fine focus knob slightly
to have a clear view of the specimen. As the magnification is increased, the
user may also find adjustments in the various methods of regulating the
brightness (see below) will improve the view of the specimen.
It is important to remember when adjusting the focus that the objective
should never touch the specimen. When increasing to 100x magnification, the
objective will appear to be very close to the slide. Because the 100x is an oil
immersion objective, a drop of immersion oil should be applied in the gap
between the objective and the specimen. The 100x oil immersion objective
should be wiped off with a piece of soft clean cloth or lens tissue to remove the
immersion oil immediately after using.
This microscope is equipped with a “locking nut” (located behind the stage
against the arm of the stand) to help prevent the stage from moving up far
enough to collide with the objectives. This locking nut is pre-set in position at
the factory and should not require adjustment. If you find that the stage is not
moving up far enough to get your specimen in focus, try turning the locking
knob slightly to allow the stage to travel farther.
Regulating brightness of the view
:
There are several ways to adjust the intensity of light shining through the
specimen, in order to see things more clearly. In most cases, a better view is
obtained by the use of a coloured glass filter inserted below the iris diaphragm.
Slide the filter holder to the right to reveal an open ring. Insert the blue filter into
this ring and slide the filter holder back into position under the diaphragm.
The iris diaphragm below the stage fitted underneath the condenser can be
opened (slide lever back to left) or closed (slide lever forward) to control the
amount of light directed through the condenser.
