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Neurotechnology Research Systems

Optogenetic Controller

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Optogenetic Controller

User’s Guide December 2012

CAUTION:

THIS OPTOGENETIC CONTROLLER IS NOT FOR USE IN HUMANS

CAUTION:

READ THE ENTIRE MANUAL BEFORE ATTEMPTING TO OPERATE THIS EQUIPMENT

Plexon Inc Proprietary

The information contained herein is the property of Plexon Inc. and it is proprietary and restricted

solely to assist Plexon Inc. customers.

Neither this document nor the contents may be disclosed, copied, revealed or used in whole or in

part for any other purpose without the prior written permission of Plexon Inc. This document must

be returned upon request of Plexon Inc.

The information contained in this document is subject to change without notice. Plexon Inc.

reserves the right to make changes in equipment design or components as progress in engineering

or manufacturing may warrant.

This user’s guide is PN 08-06-A-56-P11

This user’s guide refers to:

Hardware

PN 08-06-A-04

Firmware

PN 08-06-A-32

Software version

1.0.0

® Plexon is a registered trademark of Plexon Inc.

Printed in the United States of America

Содержание Optogenetic Controller

Страница 1: ...2011 Plexon Inc 6500 Greenville Ave Suite 700 Dallas Texas 75206 United States of America Tel 214 369 4957 Fax 214 369 1775 E mail info plexon com www plexon com Optogenetic Controller December 2012 O...

Страница 2: ...opied revealed or used in whole or in part for any other purpose without the prior written permission of Plexon Inc This document must be returned upon request of Plexon Inc The information contained...

Страница 3: ...true in Windows 7 9 5 1 7 An Opal Kelly FrontPanel USB Driver Setup window will pop up 9 5 1 8 Accept the Opal Kelly License Agreement 10 5 1 9 Click Finish When the Opal Kelly Driver Setup is complet...

Страница 4: ...Stimulation 16 6 6 Defining a pattern with a txt file 18 6 7 Loading a pattern from a txt file 18 6 8 Verifying the output on an oscilloscope 20 6 9 Using Different Scaling Options 20 7 GUI Function...

Страница 5: ...EDs 26 8 5 1 Device 26 8 5 2 I on V off 26 8 5 3 Running 26 8 5 4 Non Zero 26 8 6 Digital Out 27 8 7 Stimulation Cables 27 9 Sample Arbitrary Waveform Pattern Files 27 10 Optogenetic Controller Limita...

Страница 6: ...its may be defined with 8 bit precision and output waveforms may be defined with 8 bit resolution within those output limits Playback of pulses and arbitrary stimulation waveforms may be initiated fro...

Страница 7: ...08 06 A 04 6 Color coded Insulated BNC Cables 4 Plexon 06 03 A 04 CCLL International customers The stimulator power supply has an International Electrotechnical Commission IEC 60320 C14 inlet for AC...

Страница 8: ...Browse to the Optogenetic Contrller Radient_x64 or Radient_x86 folder on the USB flash drive depending on if you have a 64 or 32 bit operating system 5 1 2 Right click the file Radientx86v1Setup exe...

Страница 9: ...ntinue 5 1 5 You will be asked to confirm where the files will be stored Click Next to continue 5 1 6 When the installation begins it may take a couple of minutes before the progress bar begins moving...

Страница 10: ...Click Next 5 1 8 Accept the Opal Kelly License Agreement 5 1 9 Click Finish When the Opal Kelly Driver Setup is complete 5 1 10 Click Finish in the Plexon Radiant installation window 5 1 11 The compu...

Страница 11: ...ed for viewing the output of the channels in voltage mode 5 2 1 Connect the AC power cord between the AC outlet and the power supply 5 2 2 Connect the DC power cord between the power supply and the Op...

Страница 12: ...e first time you turn the stimulator on a Found New Hardware balloon will appear in the lower right hand corner of the computer screen In Windows 7 the balloon changes from Installing device driver so...

Страница 13: ...s highly recommended that you work through this section before attempting to do any type of stimulation Note that you will need an oscilloscope to see the output 6 1 Overview Every optogenetic stimula...

Страница 14: ...perational Flowchart Start Radiant Software Set LED or LASER Mode Set Max Curent Voltage or Power Pattern Manual Enter Desired Current or Voltage Pattern File Browse to File No File Header Enter Info...

Страница 15: ...ou may load the factory default configuration at any time by clicking File Restore Factory Settings Note that if the USB cable is not connected to the controller or if the controller power is turned o...

Страница 16: ...Note that a pulse started from software can be stopped with a digital input and vice versa In software until a pattern for a channel has been defined the start button in the top left of the interface...

Страница 17: ...pattern the pattern will be cut short A start signal that occurs after a pattern has been cut short will resume playback of the pattern When a stimulation pattern plays to completion the Radiant softw...

Страница 18: ...software such as Matlab or Labview The text file is simply a list of amplitudes that is updated every 100 s To maintain a value longer than 100 s the value is repeated Several sample files are includ...

Страница 19: ...output mode is in mW you should not select Voltage or current as your file s units After you select the units the channel is ready and the start button becomes active A preview will appear on the left...

Страница 20: ...Scaling Options The Optogenetic Controller fundamentally outputs either a 0 5V signal to control a laser or a 0 1100mA signal to directly power an LED The Radiant software allows patterns to be speci...

Страница 21: ...e being output on an oscilloscope You will see that it is 2 5V 5V 20mW 40mW 7 GUI Function Reference 7 1 Status The status of a given channel is either Not Ready or Ready Until a pattern for a channel...

Страница 22: ...r current mode respectively 7 5 2 File Arbitrary waveform patterns are stimulation patterns that are defined in text files with a opt extension From the Pattern drop down list select File Then click o...

Страница 23: ...xt 10 kHz clock edge comes following the digital input Stopping stimulation also has a 100 s variability because the pulse cannot end until a 10kHz clock edge is seen Once the stimulation pattern is t...

Страница 24: ...e restored by selecting File Restore Factory Settings You can also go to Window Layout Reset to Default Layout to set the windows of the GUI back to their default state 7 9 View When one controller is...

Страница 25: ...power supply 8 2 Digital In Each channel in the stimulator has a dedicated digital input DI that can initiate stimulation on that channel See sections The inputs are TTL compatible 8 3 USB 2 0 The sti...

Страница 26: ...GUI The LED above 1 will illuminate for controller number 1 the LED above 2 will illuminate for controller number 2 etc 8 5 2 I on V off Each channel has a dedicated LED to indicate if it is outputti...

Страница 27: ...ouching This is particularly useful when using the controller in current output mode The circuit that sets the current output relies on measuring the return current so if the outer BNC contact were sh...

Страница 28: ...ltage output is less than 4us Because all channels operate on the same 10kHz clock there is 100 s of variation in start latency when starting a second channel while the first is running This is becaus...

Страница 29: ...s had longer fall times than orange ones These delays are still small relative to the kinetics of the opsins that are available at this time 10 2 Synchronization of multiple Controllers The Radiant GU...

Страница 30: ...LED is connected to the controller while it is in voltage mode and the output is switched to current mode a brief pulse of light approximately 4ms long will occur as the current output stabilizes at...

Страница 31: ...Current or Voltage 4 3mA 19 5mV Output Resolution Max current or voltage 256 Output accuracy with max set to 5V for outputs 800 mV error less than 10 mV for outputs 800 mV error less than 1 Output acc...

Страница 32: ...Plexon Inc 6500 Greenville Ave Suite 700 Dallas Texas 75206 United States of America Tel 214 369 4957 Fax 214 369 1775 E mail info plexon com www plexon com Optogenetic Controller December 2012...

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