PerkinElmer IVIS Lumina III Series, Руководство по оборудованию

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IVIS ® Lumina XRMS Series III Hardware Manual Chapter 8 | Fluorescence Module
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Following the excitation filter, a second lens focuses light into a one quarter inch fused silica fiber
optic bundle inside the IVIS Lumina XRMS Series III imaging chamber. Fused silica (core and clad)
fibers are used in this bundle to avoid the generation of auto-fluorescence in the fiber, as is the case
with ordinary glass fibers.
The fused silica fiber bundle splits into four separate legs that deliver filtered light to four reflectors
located on the ceiling of the imaging chamber (
Figure 8.2 on page 59 ). Typical illumination profiles
for stage locations A-D (fields of view 5-12 cm respectively) are shown in Figure 8.7 . Note that the
profiles for all the stage locations are peaked near their center. The non-uniformity of the
illumination pattern is compensated for when units of efficiency are selected in the Living Image
software (for more details, see the Living Image® Software User's Manual). When imaging 96-well
plates, the lower stage positions (C and D) are recommended to minimize shadowing effects due to
the off-axis illumination. Fluorescent emission from the target fluorophore is collected through an
emission filter wheel located at the top of the imaging chamber and then focused into the CCD
camera. The emission filter wheel contains eight openings. Users have the ability to choose up to
seven emission filters (60 mm diameter), leaving one position open for bioluminescent imaging.
Figure 8.6
Excitation Filter Wheel – Cross Sectional Area
Figure 8.7
Typical Illumination Profiles for Stage Locations (FOV of 5, 7,
10 and 12 cm, respectively) Measured From the FOV Center