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IVIS Spectrum

In Vivo

and

In Vitro

3D Optical Imaging

Bioluminescence and Fluorescence

Spectral Unmixing

Transmission and Reflectance Imaging

Hardware Manual

March 2012

Содержание Caliper IVIS Spectrum

Страница 1: ...IVIS Spectrum In Vivo and In Vitro 3D Optical Imaging Bioluminescence and Fluorescence Spectral Unmixing Transmission and Reflectance Imaging Hardware Manual March 2012 ...

Страница 2: ...marks or trademarks of Xenogen Corporation The names of companies and products mentioned herein may be the trademarks of their respective owners Apple Macintosh and QuickTime are registered trademarks of Apple Computer Inc Microsoft PowerPoint and Windows are either registered trademarks or trademarks of Microsoft Corporation in the United States and or other countries Adobe and Illustrator are ei...

Страница 3: ...m Components 7 2 7 Power Considerations 8 2 8 Cleaning or Moving the System Components 9 2 9 Other Equipment Chemicals or Supplies 9 2 10 Servicing 10 3 Warnings 11 3 1 Electrical Safety 11 3 2 Burn Hazard Eye Safety 12 3 3 Mechanical Safety 12 3 4 Chemical Biological Safety 15 3 5 Panels Covers Modules 15 4 Legal Notices 17 4 1 Limited Warranty 17 4 2 Patents 18 4 3 Trademarks 18 5 Specifications...

Страница 4: ...System 35 7 2 Gas Plumbing 37 7 3 Door Operation 39 7 4 Centering a Subject in the Field of View 39 7 5 Fluorescent Imaging 41 7 6 Imaging Basics 42 7 7 System Shut Down Procedure 42 8 Troubleshooting 45 8 1 Measured Temperature Does Not Equal the Demand Temperature 45 8 2 Photographic Image Is Unacceptable 46 8 3 Luminescent Image Is Unacceptable 47 8 4 No Image Is Produced 48 8 5 Hardware Proble...

Страница 5: ... Chiller Unit 57 10 1 Required Tools 57 10 2 Turning Off Electric Power to the Main Console 57 10 3 Removing the Console Rear Panel 58 10 4 Sliding the Tray Out 59 10 5 Filling the ThermoCube Unit 60 10 6 Reassembling the System 61 10 7 Restarting the IVIS Spectrum 61 Appendix A Spare Parts List 63 ...

Страница 6: ...Contents iv ...

Страница 7: ...es In the reflectance mode light is delivered to four reflectors that are located on the ceiling of the imaging chamber In the transillumination mode the excitation light is delivered to an x y translation assembly under the stage and focused to a 2 mm diameter beam that can be directed to a particular location on the underside of the animal subject The system includes ten excitation and 18 emissi...

Страница 8: ... how to operate and maintain the equipment and provides guidelines for obtaining the best bioluminescent or fluorescent images Before using the IVIS Spectrum please read this manual carefully to obtain safe optimum performance and a maximum service life from the unit For instructions on using the system software please see the Living Image Software User s Manual If you have questions regarding thi...

Страница 9: ...tems Technology Support For technical support please contact Caliper at Telephone 1 877 522 2447 US 1 508 435 9500 E mail tech support caliperls com Fax 1 508 435 3439 Mail Caliper Life Sciences US Corporate Headquarters 68 Elm Street Hopkinton MA 01748 ...

Страница 10: ...1 Welcome 4 This page intentionally blank ...

Страница 11: ...ect the performance of the IVIS Spectrum If the equipment is used in a manner not specified in this manual the protection provided by the equipment may be impaired CAUTION CAUTION This symbol indicates a potentially hazardous situation which if not avoided may result in minor or moderate injury and or mechanical damage It is also used to alert against unsafe practices It reminds you that all safet...

Страница 12: ...operating instructions Failure to adhere to warnings or safety precautions may void any warranty covering this product Label Description Laser caution label Note The actual warning label mounted on the laser module at the service access inside the unit uses non fluorescing colors and materials The Caliper IVIS Spectrum Imaging Instrument contains a Class 3R Laser module The instrument itself is cl...

Страница 13: ...s source 2 6 Environmental Considerations for the System Components Location for the IVIS Spectrum Before the IVIS Spectrum is installed consider the proper environment for the components Install the equipment in an environment where The temperature does not change or fluctuate widely Choose an environment where the temperature is maintained between 15 25 C 59 78 F The humidity does not exceed 80 ...

Страница 14: ...8 Gas Anesthesia System Make sure that all equipment is plugged into a properly grounded AC supply The computer should share the same circuit to avoid ground loops Never use auxiliary power outlets on other equipment to supply the IVIS Spectrum Since the system contains internal surge protection it must be powered directly from the main electrical supply wall socket Use the surge protector provide...

Страница 15: ...nts Sprays and liquids that come into contact with the IVIS System may result in damage to the system or electrocution For more details on proper care of the system see Cleaning the IVIS Spectrum page 51 Moving the IVIS Spectrum The IVIS Spectrum is a sensitive scientific instrument and should be moved with care Pay particular attention when rolling the instrument on its casters to avoid toppling ...

Страница 16: ...ervicing to IVIS Imaging Systems Technology Support If the product is damaged and requires service unplug the product from the outlet and contact technical support Servicing by anyone other than those authorized by Caliper voids any warranty covering this product ...

Страница 17: ...t IVIS Imaging Systems Technology Support for electrical service needs Although there are no voltages in excess of 45V inside the imaging chamber local line voltages can be present inside the electronics cabinets CAUTION CAUTION If cleaning is necessary wipe the exterior surfaces of the IVIS Spectrum with a soft damp cloth only Do not allow fluids of any kind to enter the system interior under any...

Страница 18: ...s and jewelry before placing hands in the imaging chamber Complies with FDA performance standards for laser products except for deviations pursuant to Laser Notice No 50 dated 6 24 2007 Complies with IEC EN 60825 1 2007 Light Source Module The IVIS Spectrum is equipped with a fluorescence light source module and connecting fiber optic cables that are capable of producing intense light that could b...

Страница 19: ... Imaging Systems Technology Support The IVIS Spectrum contains several delicate optical and mechanical components Do not touch these components and avoid sprays or other contaminates that may damage lenses or other optical mechanical components CAUTION CAUTION The laser galvanometer is a very delicate optical component that is visible when the filter wheel side access panel is removed Figure 3 3 D...

Страница 20: ...pening at the top of the chamber Never place any object tool or fingers into this opening otherwise damage to the galvanometer will result WARNING WARNING DO NOT look directly into this opening when the laser is operating Figure 3 3 Filter wheel removable panel Filter wheel panel ...

Страница 21: ...echnology Support Ask your laboratory safety officer to advise you about the level of containment required for your application and about the proper decontamination or sterilization procedures to follow Handle all infectious samples according to good laboratory procedures and methods to prevent the spread of disease 3 5 Panels Covers Modules The imaging chamber door and the filter wheel access pan...

Страница 22: ...3 Warnings 16 This page intentionally blank ...

Страница 23: ... conditions apply a The System has been subjected to unauthorized modifications e g unauthorized installation of hardware or software unauthorized repair or servicing misuse neglect abuse accident alteration any use inconsistent with or in contradiction to the instructions in this manual or other acts which are not the fault of Caliper b Caliper was not advised in writing by the Customer of the al...

Страница 24: ...2 Patents The detection and imaging of light originating within mammals is the subject of several issued patents and pending patent applications in the United States and around the world including U S Patent Numbers 5 6200 135 6 217 847 6 649 143 6 890 515 and European Patent Commission Number EP0861093 for which Xenogen Corporation is the exclusive licensor The use of an IVIS Imaging System for s...

Страница 25: ...ion in the United States and or other countries Adobe and Illustrator are either registered trademarks or trademarks of Adobe Systems Incorporated in the United States and or other countries Intel is a registered trademark of Intel Corporation ...

Страница 26: ...4 Legal Notices 20 This page intentionally left blank ...

Страница 27: ...ed Dedicated Circuit 100 120VAC 50 60Hz 15A 220 240VAC 50 60Hz 10A 20 Amps for 120VAC or 10 Amps minimum for 230VAC For more details see Power Considerations page 8 CCD Camera Specification Sensor Type Back illuminated CCD Format 2048 x 2048 pixels CCD Size 26 x 26 mm Effective CCD Format 1920 x 1920 pixels Pixel Dimensions 13 5 x 13 5 μm Quantum Efficiency 85 400 700nm 50 350 900nm Readout Noise ...

Страница 28: ...ng Component Specification Excitation Filters 12 positions 25 mm diameter 10 filters supplied Emission Filters 22 positions 60 mm diameter 18 filters supplied Background Autofluorescence and Leakage 1 part in 107 typical Lamp 150 W quartz halogen 21 V 3250 Kelvin Environmental Component Specification Temperature 15 25 C 59 78 F Humidity 0 80 non condensing Type of Use Indoor Sound Level 61 dB 500 ...

Страница 29: ...rum Hardware Manual 5 6 Weights Dimensions Item Specification Weight 275 kg 600 lbs Depth 77 cm 30 inches Width 65 cm 25 5 inches Height 211 cm 83 inches Imaging Chamber Internal Dimensions 51 x 51 x 66 cm D x W x H ...

Страница 30: ...5 Specifications 24 This page intentionally blank ...

Страница 31: ...Laser galvanometer Photographic illumination system Integrated fluorescence system Imaging platform crash avoidance system These components are integrated into one freestanding instrument that is equipped with casters Figure 6 1 A pre configured Windows based computer enables you to control the system as well as acquire and analyze images IMPORTANT ALERT If you modify the IVIS Imaging System in an...

Страница 32: ... has a 16 bit digitized low noise electronic readout for extremely low background images 6 2 ThermoCube Chiller Unit The ThermoCube unit is a thermoelectric water chiller Figure 6 2 Water from the thermoelectrically cooled CCD camera is pumped to the ThermoCube unit where the water is cooled and returned to the camera Figure 6 1 IVIS Spectrum components The system is housed in a portable cart with...

Страница 33: ...scent imaging The imaging chamber is equipped with gas anesthesia inlet and outlet ports for use with the optional XGI 8 Gas Anesthesia System The imaging chamber is light tight so that no light penetrates from the outside after the magnetically activated door is locked The magnetic latch ensures the door cannot be inadvertently opened during an imaging session The interior of the imaging chamber ...

Страница 34: ...op of the imaging chamber protects the lenses and other optical components in the lens compartment from contamination For more details on the care of this window see Cleaning the Lens Protection Window page 52 NOTICE Do not touch the glass window or permit specimens to come in contact with the window otherwise image quality may be impaired If the window is struck by a hard object it may crack or s...

Страница 35: ...rs If the filters must be changed or the module requires service contact IVIS Imaging Systems Technology Support For more details on fluorescence imaging see page 41 6 5 Specimen Warming System The temperature controlled imaging platform keeps specimens warm during imaging The temperature is controlled by the software and is only enabled when the instrument is initialized from Living Image softwar...

Страница 36: ...ured light for 3D contouring and auto focusing 6 7 Crash Avoidance System The crash avoidance system uses a scanning laser to determine the subject height and verify that it is within acceptable limits This system prevents injury to specimens and damage to delicate optical components such as the lens window or the illumination diffusers for field of view A only You are prompted at the start of eac...

Страница 37: ...accessible at the rear of the instrument and the stage connector is located inside the imaging chamber Figure 6 9 Table 6 1 shows guidelines for pin usage Figure 6 7 IVIS Spectrum back view Ventilation openings Ventilation openings Table 6 1 Connector pin usage Pin Number Function 1 to 5 These pins are optimized for transport of ECG EKG signals and use shielded cabling that maximizes the signal to...

Страница 38: ...o be used to connect to the THM 100 ECG monitor Indus Instruments Figure 6 8 Back panel connector Figure 6 9 Stage connector Table 6 1 Connector pin usage Pin Number Function Note A 15 pin D sub male mating connector is required supplied by user Note Connector type in shelf MS3119E16 26PS Mating connector required supplied by customer Manufacturer Amphenol Aerospace part no MS3126F16 26P ...

Страница 39: ...when it is imaged in a light tight and contaminant free imaging chamber As a result it is not visible in a luminescent image If a light leak or an internal phosphorescent contaminant is present in the imaging chamber the hemisphere reflects that light and is visible in a luminescent image If you suspect that there is light contamination in the imaging chamber see Light contamination External page ...

Страница 40: ...6 IVIS Spectrum Components 34 This page intentionally blank ...

Страница 41: ...nable automatic overnight electronic background measurements Periodically rebooting the computer is permissible and does not affect the camera operation Electrical Control Status Lights The IVIS Spectrum console has two electrical switches and one status light Figure 7 1 The color of the status light indicates the state of the instrument Table 7 1 The main power on off switch circuit breaker is lo...

Страница 42: ...ower switch on the rear panel The status light is blue 4 Turn on the front panel toggle switch by pulling the toggle switch out and up The status light is blue and blinking Figure 7 1 IVIS Spectrum Table 7 1 IVIS Spectrum status light modes Status Light Is Indicates Blue The system is in standby mode Blue and blinking The CCD camera cooling system is disabled due to a power interruption Use the Li...

Страница 43: ...System control panel in the Living Image software The instrument is ready for operation when the camera temperature is locked at 90 C as indicated by the green temperature square in the IVIS System Control panel For operating instructions see the Living Image Software Manual The temperature square is red at startup and turns green when the operating temperature is reached Click the temperature squ...

Страница 44: ...with plumbing connections for supplying isoflurane anesthetic gas to the imaging chamber and exhausting gases out The flow of gas into the imaging chamber is manually controlled by the gas valve on the front panel of the console The imaging chamber does not include a gas scavenging system This is provided by suitable auxiliary equipment such as the XGI 8 Gas Anesthesia System Gas ports are located...

Страница 45: ... as the XGI 8 Gas Anesthesia System is used 7 3 Door Operation The door of the imaging chamber cannot be opened when the system status light is red The door is equipped with an electromagnetic lock to prevent accidental opening during an imaging session WARNING WARNING Never try to defeat the door interlock or force the door open during image acquisition when the status light is red Such action co...

Страница 46: ...d select a different FOV setting For example FOV settings see Figure 6 4 page 28 NOTICE The alignment laser turns off after 60 seconds Repeat step 1 to turn on the laser 4 Using the alignment grid as a guide place the specimen s at the locations of interest then proceed with image acquisition NOTICE The Living Image software controls the photographic illumination LED intensity Figure 7 4 IVIS Syst...

Страница 47: ...witch that directs the light to either the top of the imaging chamber or to the bottom of the stage When top illumination is selected the light is equally split to four diffusers to provide uniform stage illumination When bottom or trans illumination is selected the excitation light is directed to one of the 2 mm diameter holes in the aperture plate located in the stage surface This removable plat...

Страница 48: ... that nearly EVERYTHING glows that is has the potential to phosphoresce and contaminate the image Most plastics almost all tape plants paint rodent food mostly plants mouse urine and animal bedding have been found to glow Use caution when introducing materials into the IVIS Spectrum It is advisable to prescreen all items by imaging them alone before imaging them with samples under study Caliper re...

Страница 49: ...wall socket 6 Cover the gas inlet and outlet ports with masking tape to prevent entry of dust 7 Remove any loose objects from the imaging chamber and close the door If you plan to move the system see important information on page 55 If you have any problems during the shut down procedure please contact IVIS Imaging Systems Technology Support for assistance ...

Страница 50: ...7 Operating the IVIS Spectrum 44 This page intentionally blank ...

Страница 51: ...e chiller unit remain on the system maintains this temperature regardless of whether the Living Image software is open or the computer is turned on To check the temperature of the CCD click the Temperature square red or green in the Camera Control Panel of the Living Image software Figure 8 1 Figure 8 1 IVIS System control panel in the Living Image software Click the temperature square to display ...

Страница 52: ...hesia is being used confirm that the anesthesia is turned on and the flow rate is appropriate Image is blurry Subject height is not correctly entered in the Living Image software If you are using the Sample Height setting to determine the focus confirm that the correct height of the sample is entered Alternatively Auto Focus may be used to determine the sample height If you are using the Manual se...

Страница 53: ...eflectance hemisphere can be special ordered to help check for light leaks XRH 1 part number 118937 Figure 8 3 To check for light leaks 1 Place the high reflectance hemisphere in the imaging chamber on the stage using a subject height of 3 5 cm at field of view D 2 Take a luminescent image of the hemisphere using the luminescent settings f stop 1 Binning Large high sensitivity and exposure time 5 ...

Страница 54: ...e main power switch should not trip Frequent tripping of this circuit breaker may indicate an underlying electrical problem If this occurs unplug the system and contact IVIS Imaging Systems Technology Support If the circuit breaker is occasionally tripped 1 Confirm that the rear panel switch is off the front panel toggle switch is down standby and the power cord is plugged into the console and the...

Страница 55: ...perature before you begin an imaging session Laser Galvanometer The laser galvanometer is not user serviceable If you notice any changes to the alignment grid for example change in the brightness or position of the grid contact IVIS Imaging Systems Technology Support Lamp Replacement The quartz halogen lamp that provides the fluorescent light source typically operates for thousands of hours This l...

Страница 56: ... moves to the service position and the door can be opened 3 Place the stage lock down bar in position as shown in Figure 8 5 Be careful not to disturb the platform heater cable or the gas tubing located in this area To return the platform to the operating position 1 Return the lock down safety bar to the stowed position under the front edge of the imaging platform and close the door 2 In the IVIS ...

Страница 57: ...tem or electrocution WARNING WARNING Do not use fluids or moistened towels to clean any part of the system console where electrical or fiber optic cables make connections Do not use fluids of any kind near the filter wheel assembly Turn off the electrical power to the IVIS Spectrum by turning the front panel toggle switch to standby and turning off the rear panel switch before engaging in cleaning...

Страница 58: ... that the surfaces are dry before using the imaging chamber 9 2 Cleaning the Lens Protection Window Contact IVIS Imaging Systems Technology Support for information about cleaning or sterilizing any of the optical components or replacing the optical filters A clear window at the top of the imaging chamber protects the lenses and other optical components from contamination Do not touch or allow subj...

Страница 59: ...the inner left hand side towards the front of the console Noting this orientation is helpful when you reinstall the panel 4 Notice that the 1 2 shaft on the right side of the filter wheel compartment has a carriage clamp lock with a handle Turn the handle counter clockwise to loosen the clamp so that it can slide freely over the shaft NOTICE Starting with step 5 wear lint free powderless gloves to...

Страница 60: ... arrow that is printed on the side of the filter points up 9 Push the filter wheel carriage back into the console by placing one finger on the rim of the upper filter wheel 10 While you hold the carriage in the closed position turn the carriage locking clamp in the clock wise direction Be careful not to dislodge any drive belts off of their pulleys when locking the clamp 11 Replace the access pane...

Страница 61: ... panel toggle switch in the standby position 2 Turn off the main power switch on the rear panel and disconnect the power cord from the wall socket and the console 3 Remove all cables from the rear panel of the console CAUTION CAUTION Any cables that dangle from the console could cause the system to topple if caught by a caster 4 Unlock the four casters 5 Grip the console at a convenient location b...

Страница 62: ...aintenance 56 The status light is red The status light turns green when the system is initialized Wait until the camera has cooled to the demand temperature before you begin an imaging session about ten minutes ...

Страница 63: ...quired but not provided Allen Wrenches hex head wrench 3 32 and measured across the flats AF Measuring cup with a spout Spectral TE Camera Cooling Mixture part no 119219 includes a 3 32 and allen wrench or a solution of 25 pure ethanol 75 distilled water Paper towel or cloth for clean up 10 2 Turning Off Electric Power to the Main Console VOLTAGE VOLTAGE Do not remove any panels from the console u...

Страница 64: ... lean it against the side of the console CAUTION CAUTION Be careful not to pull too hard on the rear fan panel otherwise its plug may become disconnected If this happens contact IVIS Imaging Systems Technology Support Figure 10 1 Lower rear panel Note that the power cord has been removed from the console Figure 10 2 Removing the lower rear panel using a hex head wrench left and ThermoCube unit exp...

Страница 65: ...y 3 Pull out the tray with the ThermoCube unit Figure 10 4 NOTICE The ThermoCube unit has its own power switch located at the rear of the unit The power switch is not visible until the unit is pulled out Make sure that the power switch is not accidentally turned off when you slide the tray out or back into the console 4 Remove the fill cap on the ThermoCube chiller and check coolant level If the c...

Страница 66: ...ION DO NOT OVERFILL the ThermoCube unit Over filling the tank can cause fluid to run down the chiller walls and contact the power plug NOTICE If the Caliper Spectral TE Camera Cooling Mixture part no 119219 is not available a suitable substitute can be made from ordinary 80 proof Vodka and distilled water To make 750 ml of the disinfecting coolant mix 470 ml of 80 proof alcohol 40 ethanol and 280 ...

Страница 67: ...ews Figure 10 2 10 7 Restarting the IVIS Spectrum 1 Confirm that the IVIS system rear panel power switch is off and that the front panel toggle switch is in the down standby position 2 Reconnect the power cord first to the console and then to the wall socket 3 Confirm that all other cables are properly connected 4 Turn on the computer 5 Turn on the rear panel power switch The front status light is...

Страница 68: ...10 Servicing Maintaining the ThermoCube Chiller Unit 62 This page intentionally blank ...

Страница 69: ...escription Caliper Part Number Mouse Inhalation Nose Cone 117150 Low Profile 5 Station Manifold 117706 XRH 1 High Reflectance Hemisphere with container 118937 Black Paper Swarthmore Artagain 9x12 24 sheet pad 117837 Low Fluorescence Imaging Mat 11 6 square 117839 ...

Страница 70: ...A Spare Parts List 64 This page intentionally blank ...

Страница 71: ...ing 5 dome 55 door operation 39 E electrical power requirements 21 electrical safety 11 emission filter selecting 41 emission filter wheel 29 emission filters changing 53 54 environmental considerations 7 heat 7 location of system 7 space and ventilation 8 water and moisture 8 environmental requirements 22 excitation filter selecting 42 excitation filter wheel 29 eye safety 12 F field of view cent...

Страница 72: ...3 O optical components lenses 28 optical specifications 22 other equipment chemicals or supplies 9 P patents 18 photographic image unacceptable 46 pinch hazard label 6 power cord 8 power line surges 8 power outages 9 power overloading 9 power sources 8 Q quartz halogen lamp 49 R read manual label 6 restarting the system 35 37 S safety burn hazard 12 chemical biological 15 electrical 11 eye 12 lase...

Страница 73: ...arks 18 transillumination 41 troubleshooting camera noise 47 light contamination 47 measured temperature does not equal demand temperature 45 no image produced 48 streaked image 46 unacceptable luminescent image 47 unacceptable photographic image 46 V voltage definition 5 W warming system 29 warning definition 5 water and moisture safety 8 weights and dimensions 23 X XGI 8 Gas Anesthesia System 38...

Страница 74: ...Index 68 ...

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