Operating Basics
Rev. 007 Date 2010-01-13
CyFlow
®
space Instrument Operating Manual
11/26
Optical Standard Setup
– The Parameters
Note
If required by a specific application, the optical
standard setup can be optimized by exchanging
preassembled removable mirror/filter blocks. This
is a matter of seconds and does not require
readjustments.
Fig. 2: Examplary demonstration of the optical
bench of a CyFlow
®
space with 2 lasers and 8
parameters (top) and 3 lasers and 9 parameters
(bottom). Other laser light sources and parameter
allocations possible. D detector (photo multiplier)
The CyFlow
®
space flow cytometer can be
equipped with various laser light sources (1, 2 or 3
lasers), different laser power intensities and up to
nine optical parameters.
Due to
its
modular
concept
the
optical
configuration can be adopted to many different
clinical and scientific purposes. The main
standard configurations include:
2 Laser instrument:
This instrument is equipped
with a blue diode pumped solid-state Laser (20
mW) at 488 nm and a red laser diode at 638 nm
(25 mW). Up to eight optical parameters can be
included (colours reflect laser origin of the signal).
FSC:
forward scatter
SSC:
side scatter
FL1:
green fluorescence
(FITC)
FL2:
orange fluorescence
(PE)
FL3:
red fluorescence I
(PE-Cy5)
FL4:
far red fluorescence I
(PE-Cy7)
FL5:
red fluorescence II
(APC)
FL6:
far red fluorescence II
(APC-Cy7)
Other parameter allocations possible
3 Laser instrument, high power laser 488 nm:
This instrument is equipped with a blue diode
pumped solid-state Laser (200 mW) at 488 nm, a
violet laser diode at 405 nm (100 mW) and a red
laser diode at 638 nm (25 mW). Up to nine optical
parameters can be included (colours in the table
below reflect laser origin of the signal).
FSC:
forward scatter
SSC:
side scatter
FL1:
green fluorescence
(FITC)
FL2:
orange fluorescence
(PE)
FL3:
red fluorescence I
(PE-Cy5)
FL4:
far red fluorescence I
(PE-Cy7)
FL5:
red fluorescence II
(APC)
FL6:
far red fluorescence II
(APC-Cy7)
FL7:
blue fluorescence
(CFP, Alexa 405)
Other parameter allocations possible
Other lasers available:
532 nm green solid state laser (50 or 100 mW)
375 nm UV laser diode (16 mW)
More lasers on request
The instrument is equipped with a CCD camera in
order to monitor the particle flow and to check the
focus of the objective.
Laser
488 nm
Laser
405
nm
Laser 638 nm
Flow cell
M
M
M
D
FSC
SSC
FL3
FL1
FL4
FL5
FL2
FL6
Laser
optic
CCD
D
D
D
D
D
D
D
FL7
D
Laser
488 nm
Laser 638 nm
Flow cell
M
M
D
FSC
SSC
FL3
FL1
FL4
FL5
FL2
FL6
Laser
optic
CCD
D
D
D
D
D
D
D
Содержание CyFlow space
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