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5.
Using the Cadence ILC Module
This section describes how to complete the procedures in each of the four parts of an SPTFF process,
and is organized as follows:
• Preparation of Equipment and Materials
• Part 1 — Pre-Use Conditioning: Procedures A through H
Pre-use conditioning establishes the fitness of the module for use.
• Part 2 — Processing: Procedures I through J
• Part 3 — Post-Use Conditioning (optional): Procedures K to O
• Part 4 — Post-Use Disposal
5.1
Preparation: Equipment and Materials
Equipment
• Cadence ILC module
• SPTFF set-up consisting of (at minimum) a feed pressure source and feed pressure sensor,
and a permeate 1 pressure sensor. Typically, end-users also have retentate and/or permeate
flowmeters (or scales) to monitor their process.
• Appropriate tubing set. Pall can provide tubing sets for your application, please contact your
Pall representative for more details
• Containers to hold and collect product, water, buffer, and sanitizing solutions
• Tubing and connectors:
– For a Cadence system with Cadence T01 or T02 modules: Female Luer type connectors
and
⅛
in. internal diameter (ID), that needs to withstand 4.1 barg (60 psig)
– For a Cadence system with Cadence T12 modules: MPC quick disconnect fittings,
¼ in. ID, that needs to withstand 4.1 barg (60 psig)
– For a Cadence system with Cadence T06 modules: MPX quick disconnect fittings,
½ in. ID, that needs to withstand 4.1 barg (60 psig)
• Personal protective equipment as required by OSHA (U.S. Occupational Safety and Health
Administration) and your facility’s safety regulations
• pH meter
• Palltronic
®
Flowstar integrity test instrument
• Thermometer
Materials
• Product solution
• Buffer solution compatible with product: 15 L/m
2
• De-ionized, 0.2 μm filtered water: 40 L/m
2
(for pre-conditioning steps)
• Sodium hydroxide: 45 L/m
2
(for pre-conditioning sanitization)
– Delta membranes: 0.1 – 0.25 N
– Omega membranes: 0.1 – 0.5 N
• FOR REUSE: Post-conditioning cleaning agent, 50 L/m
2
– Delta membranes: 0.1 N sodium hydroxide
– Omega membranes: 0.5 N sodium hyd 400 ppm sodium hypochlorite
• FOR REUSE: Storage solution, 10 L/m
2
– Delta membranes: 0.1 N sodium hydroxide (< 3 months); 5% ethanol, 1% sodium
diacetate (> 3 months)
– Omega membranes: 0.1 N sodium hydroxide
HELPFUL INFORMATION:
Solution volumes are provided as a guide. Appropriate volumes
must be determined by users for their specific applications.
