Oroboros Instruments O2k-FluoRespirometer Скачать руководство пользователя страница 7

MiPNet22.11 

O2k-FluoRespirometer manual

 

7

 

 

 

Oroboros Instruments

 

 

High-Resolution FluoRespirometry

 

 

5.3. O2k OroboPOS assembly 

 

 

The POS are shipped dry without electrolyte in 

a Perspex housing in the OroboPOS-Service Kit. 
Before  assembling  the  O2k,  apply  electrolyte 

and membrane, following the OroboPOS service 
instructions. 

 

Go to: OroboPOS assembly

  

» 

MiPNet19.18B_POS-service

 

 

 

 

1

.  Screw  a  POS  head  onto  each 

POS  connector

 

(finger-tight). Push a 

wetted

 POS seal tip (black gasket) 

over the POS head (arrow 1). Position the pore centrally 
and do not stretch the gasket. 

 

 

2

.  Connect  the  POS  connector  cable  to  the  O2k-Main 

Unit. Insert the male plug of the cable 2 into the female 
O2 plug. The red dot on the male plug  2 faces straight 
upwards.  Each  OroboPOS  head  can  be  used  on  O2k-

chamber A or B. Note the POS number (marked on 
the cylindrical body of each POS) for each chamber 

(see 

Section 6

)

 

3

.  After  inserting  the  plug  of  the  POS 

connector  cable  to  the  O2k-Main  unit, 
the POS connector cable 3 must be in a 
relaxed, undistorted position. Press the 
sleeve downwards  (arrow  4), insert the 
POS  with  the  black  gasket  straight  into  the  POS 
holder 5, and release the sleeve for fixing the POS 
in its final position. 

 

Do not rotate the POS connector

 6 after it 

is attached to the POS holder in order to prevent 

damage of the black gaskets. The gaskets provide 
a  tight  seal  against  the  glass  chamber  that  has 
sharp edges. 

 
 

 
 

 

 

 

Clip A2

 

Содержание O2k-FluoRespirometer

Страница 1: ...meter provides a unique high resolution approach to the monitoring of cellular and mitochondrial respiratory function High Resolution FluoRespirometry HRFR is indispensable for application of SUIT pro...

Страница 2: ...y step instructions integrated as DL Protocols into DatLab 7 Numerous new O2k Publications on applications of the Oroboros O2k focus on critical issues of mitochondrial physiology While the Oroboros O...

Страница 3: ...Titration Set 1 Unpack the O2k FluoRespirometer Box 1 or Peli Case contains the O2k Main Unit with the O2k Chambers in a finally mounted position the OroboPOS Connectors without polarographic oxygen...

Страница 4: ...ates this icon on your desktop Oroboros DatLab for control of the O2k TIP2k data acquisition analysis Running DL7 For data safety reasons and optimal DatLab performance it is strongly recommend to ope...

Страница 5: ...e for the ISS O2k Titration Set and handling For maximum signal stability and optimal temperature control the O2k should be placed in a position in which it is not exposed to direct sunlight and sudde...

Страница 6: ...ct to electrical ground or simply touch the O2k steel housing 5 1 Instrument overview The O2k FluoRespirometer is electronically equipped with all required plugs for sensors in addition to the O2 chan...

Страница 7: ...it Insert the male plug of the cable 2 into the female O2 plug The red dot on the male plug 2 faces straight upwards Each OroboPOS head can be used on O2k chamber A or B Note the POS number marked on...

Страница 8: ...cable to the Fluo plug Similar to the OroboPOS 2 insert the male plug of the cable into the female Fluo plug The red dot on the male plug faces straight upwards Each Smart Fluo Sensor can be used on...

Страница 9: ...or chambers A and B Usually sensors should not be switched between chambers except for troubleshooting The individual sensor number is included in the protocol for generating a database of calibration...

Страница 10: ...w rpm but uncoupling of the magnet occurs at very high stirring speeds causing high signal noise Both chambers may be set at an identical stirrer speed Data recording interval s can be set between 0 2...

Страница 11: ...n automatically opened Save as window the default file name has the form 2017 07 21 P1 01 DLD Year Month Day P DLD P ower O2k sequential number of the experiment extension DLD for DatLab Data Select a...

Страница 12: ...metric pressure continuously recorded Peltier power Operative Peltier power applied for temperature regulation of the O2k block continuously recorded Amp A and Amp B V Amperometric signal from amperom...

Страница 13: ...is on Illum on highlighted in yellow or off Illum off grey Toggle by a L double click When the windows to the glass chambers are closed with optical sensors the illumination is not visible and must b...

Страница 14: ...ps for renaming or deleting O2k setups 7 2 O2k control F7 with the O2k connected The O2k control key F7 provides fast access to the O2k control window Load setup After selecting a setup it must be L l...

Страница 15: ...ibed above with the next sequential number After re connecting the O2k continues operation uninterrupted the experimental time is reset to zero and data recording is restarted with automatic saving to...

Страница 16: ......

Страница 17: ...t O2k Chamber Stirrer Bar white PVDF 15x6 mm OroboPOS Holder O2k Chamber Holder A2_Insert OroborPOS OroboPOS Connector A3_O2k Chamber volume calibration Volume Calibration Ring Stopper black PEEK coni...

Страница 18: ...chamber into the copper block of the O2k FluoRespirometer 2 Screw the POS holder 1 into a position where the glass chamber with the angular cut towards the POS holder is slightly lifted upwards 3 Scre...

Страница 19: ...of the stopper black PEEK is not part of the effective chamber volume When titrating 2 1 ml insert the tip of the pipette to the wall of the glass chamber to add the entire volume Liquid must not be l...

Страница 20: ...the manual Trouble shooting of test versions DatLab 7 1Andras M sz ros Oroboros Instruments AM wrote and edited the manual Taking editing photos and images 1Ondrej Capek Oroboros Instruments OC was m...

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