Oroboros Instruments O2k-FluoRespirometer Скачать руководство пользователя страница 5

MiPNet22.11 

O2k-FluoRespirometer manual

 

5

 

 

 

Oroboros Instruments

 

 

High-Resolution FluoRespirometry

 

 

 

Note: Use O2k-Main Power Cable\230 V\Europe (Hybrid 
German/French  plug  CEE7/7,  Type  E+F)  only  for  a 

compatible socket. 

  

DK: 

Some Danish sockets will also accept the CEE7/7 plugs, 
but  there  is  no  grounding  connection  with  these  plugs 

without  male  ground  pin.  Exchange  for  an  appropriate 
Danish power cable. 

 

CH: 

A different main power cable or adapter is required. 

 

US:

 

O2k-Main Power Cable\120 V\US-CA, 21112-01 

 

AU/NZ:

 O2k-Main Power Cable\230 V\AU-NZ, 21113-01 

 

The 

O2k

 requires a minimum of lab space (0.5 m bench 

space) next to an external PC or laptop, with sufficient 
space  for  the 

ISS

O2k-Titration  Set

  and  handling.  For 

maximum  signal  stability  and  optimal  temperature 

control, the O2k should be placed in a position in which 
it is not exposed to direct sunlight and sudden changes 

of room temperature. Keep the space on the table below 
the  O2k  bottom  clear  to  prevent  any  interference  of 

ventilation beneath the 

O2k-Main Unit

 

Avoid  damage  due  to  condensation.  When  placing  the 
O2k from a cold to warm environment, let it equilibrate 

for at least 4 h in the warm room before connecting to 
the mains. 

 

 

Rear of the O2k-FluoRespirometer.

 

New features are the USB hub 2.0 with 4 external ports, 
a plug for an external Pt1000 thermistor, and an optional 

extension through an RS485 serial port. 

 

WGT quality control seal:

 Do not open. An intact quality control seal 

is  required  for  warranty  obligations  related  to  the 
electronics of the O2k. 

 

Содержание O2k-FluoRespirometer

Страница 1: ...meter provides a unique high resolution approach to the monitoring of cellular and mitochondrial respiratory function High Resolution FluoRespirometry HRFR is indispensable for application of SUIT pro...

Страница 2: ...y step instructions integrated as DL Protocols into DatLab 7 Numerous new O2k Publications on applications of the Oroboros O2k focus on critical issues of mitochondrial physiology While the Oroboros O...

Страница 3: ...Titration Set 1 Unpack the O2k FluoRespirometer Box 1 or Peli Case contains the O2k Main Unit with the O2k Chambers in a finally mounted position the OroboPOS Connectors without polarographic oxygen...

Страница 4: ...ates this icon on your desktop Oroboros DatLab for control of the O2k TIP2k data acquisition analysis Running DL7 For data safety reasons and optimal DatLab performance it is strongly recommend to ope...

Страница 5: ...e for the ISS O2k Titration Set and handling For maximum signal stability and optimal temperature control the O2k should be placed in a position in which it is not exposed to direct sunlight and sudde...

Страница 6: ...ct to electrical ground or simply touch the O2k steel housing 5 1 Instrument overview The O2k FluoRespirometer is electronically equipped with all required plugs for sensors in addition to the O2 chan...

Страница 7: ...it Insert the male plug of the cable 2 into the female O2 plug The red dot on the male plug 2 faces straight upwards Each OroboPOS head can be used on O2k chamber A or B Note the POS number marked on...

Страница 8: ...cable to the Fluo plug Similar to the OroboPOS 2 insert the male plug of the cable into the female Fluo plug The red dot on the male plug faces straight upwards Each Smart Fluo Sensor can be used on...

Страница 9: ...or chambers A and B Usually sensors should not be switched between chambers except for troubleshooting The individual sensor number is included in the protocol for generating a database of calibration...

Страница 10: ...w rpm but uncoupling of the magnet occurs at very high stirring speeds causing high signal noise Both chambers may be set at an identical stirrer speed Data recording interval s can be set between 0 2...

Страница 11: ...n automatically opened Save as window the default file name has the form 2017 07 21 P1 01 DLD Year Month Day P DLD P ower O2k sequential number of the experiment extension DLD for DatLab Data Select a...

Страница 12: ...metric pressure continuously recorded Peltier power Operative Peltier power applied for temperature regulation of the O2k block continuously recorded Amp A and Amp B V Amperometric signal from amperom...

Страница 13: ...is on Illum on highlighted in yellow or off Illum off grey Toggle by a L double click When the windows to the glass chambers are closed with optical sensors the illumination is not visible and must b...

Страница 14: ...ps for renaming or deleting O2k setups 7 2 O2k control F7 with the O2k connected The O2k control key F7 provides fast access to the O2k control window Load setup After selecting a setup it must be L l...

Страница 15: ...ibed above with the next sequential number After re connecting the O2k continues operation uninterrupted the experimental time is reset to zero and data recording is restarted with automatic saving to...

Страница 16: ......

Страница 17: ...t O2k Chamber Stirrer Bar white PVDF 15x6 mm OroboPOS Holder O2k Chamber Holder A2_Insert OroborPOS OroboPOS Connector A3_O2k Chamber volume calibration Volume Calibration Ring Stopper black PEEK coni...

Страница 18: ...chamber into the copper block of the O2k FluoRespirometer 2 Screw the POS holder 1 into a position where the glass chamber with the angular cut towards the POS holder is slightly lifted upwards 3 Scre...

Страница 19: ...of the stopper black PEEK is not part of the effective chamber volume When titrating 2 1 ml insert the tip of the pipette to the wall of the glass chamber to add the entire volume Liquid must not be l...

Страница 20: ...the manual Trouble shooting of test versions DatLab 7 1Andras M sz ros Oroboros Instruments AM wrote and edited the manual Taking editing photos and images 1Ondrej Capek Oroboros Instruments OC was m...

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