Oroboros Instruments O2k-FluoRespirometer Скачать руководство пользователя страница 4

MiPNet22.11 

O2k-FluoRespirometer manual

 

4

 

 

Oroboros Instruments 

 

Mitochondria and Cell Research 

XP or later Windows versions. Recommended: Intel i5 or 
equivalent CPU, 4GB RAM, Windows 7, SSD. 

 

 Copy  the  folder 

Oroboros  O2k-Course  on  HRFR

” 

from the 

Oroboros USB-flash drive

 to your computer. 

The 

Oroboros-FileFinder

  is  the  tool  to  find  topics  and 

open  the  files.  Updates  of  the 

O2k-Manual

  and 

O2k-

Protocols

 are posted on our website. 

 

DL7Install.exe

  

It  is  recommended  that  you  exit  all  computer 

applications  before  running  the  DatLab  installation 

programme. In the 

Oroboros-

FileFinder’, 

L

 click 

the  Oroboros  symbol  or 

‘O2k

-DatLab  i

nstall’

Read the short instruction windows, and proceed 

by  L

  Next.  By  default,  DatLab  is  installed  to 

"C:\DatLab". 

 

 

 

Installation is quick and easy and generates this icon on 
your desktop: 

Oroboros DatLab

 for control of the O2k, 

TIP2k, data acquisition & analysis. 

 

Running DL7

 

For  data  safety  reasons  and  optimal  DatLab 
performance, it is strongly recommend to operate your 

DatLab files, DL-Protocols and DatLab templates on the 

PC‘s local 

hard drive (HDD).  

 
 

MitoPedia-O2k

  is  integral  to  the  O2k-Manual,  where  specifically 

advanced sections on High-Resolution FluoRespirometry 

are included:  

 

»  

wiki.oroboros.at/index.php/MitoPedia:_O2k_hardware

 

   

»  

wiki.oroboros.at/index.php/MitoPedia:_DatLab

  

 
 

3.

 

Electric and lab space requirements 

 

 

Max. power input: 120 W 

Current at 100-120 V: max. 1.0 A 
Current at 220-240 V: max. 0.5 A 

Power  consumtion  during  standard  operation  (when 
block  temperature  is  stable  at  37  °C  and  ambient 

temperature of 22 °C): approx. 24 W 

 

Check the suitability of the delivered cable for the mains 
power (230\120 V) and socket. The O2k is delivered with 

one of three different cable types: 

  

EU:

 

O2k-Main Power Cable\230 V\Europe, 21111-01 

Содержание O2k-FluoRespirometer

Страница 1: ...meter provides a unique high resolution approach to the monitoring of cellular and mitochondrial respiratory function High Resolution FluoRespirometry HRFR is indispensable for application of SUIT pro...

Страница 2: ...y step instructions integrated as DL Protocols into DatLab 7 Numerous new O2k Publications on applications of the Oroboros O2k focus on critical issues of mitochondrial physiology While the Oroboros O...

Страница 3: ...Titration Set 1 Unpack the O2k FluoRespirometer Box 1 or Peli Case contains the O2k Main Unit with the O2k Chambers in a finally mounted position the OroboPOS Connectors without polarographic oxygen...

Страница 4: ...ates this icon on your desktop Oroboros DatLab for control of the O2k TIP2k data acquisition analysis Running DL7 For data safety reasons and optimal DatLab performance it is strongly recommend to ope...

Страница 5: ...e for the ISS O2k Titration Set and handling For maximum signal stability and optimal temperature control the O2k should be placed in a position in which it is not exposed to direct sunlight and sudde...

Страница 6: ...ct to electrical ground or simply touch the O2k steel housing 5 1 Instrument overview The O2k FluoRespirometer is electronically equipped with all required plugs for sensors in addition to the O2 chan...

Страница 7: ...it Insert the male plug of the cable 2 into the female O2 plug The red dot on the male plug 2 faces straight upwards Each OroboPOS head can be used on O2k chamber A or B Note the POS number marked on...

Страница 8: ...cable to the Fluo plug Similar to the OroboPOS 2 insert the male plug of the cable into the female Fluo plug The red dot on the male plug faces straight upwards Each Smart Fluo Sensor can be used on...

Страница 9: ...or chambers A and B Usually sensors should not be switched between chambers except for troubleshooting The individual sensor number is included in the protocol for generating a database of calibration...

Страница 10: ...w rpm but uncoupling of the magnet occurs at very high stirring speeds causing high signal noise Both chambers may be set at an identical stirrer speed Data recording interval s can be set between 0 2...

Страница 11: ...n automatically opened Save as window the default file name has the form 2017 07 21 P1 01 DLD Year Month Day P DLD P ower O2k sequential number of the experiment extension DLD for DatLab Data Select a...

Страница 12: ...metric pressure continuously recorded Peltier power Operative Peltier power applied for temperature regulation of the O2k block continuously recorded Amp A and Amp B V Amperometric signal from amperom...

Страница 13: ...is on Illum on highlighted in yellow or off Illum off grey Toggle by a L double click When the windows to the glass chambers are closed with optical sensors the illumination is not visible and must b...

Страница 14: ...ps for renaming or deleting O2k setups 7 2 O2k control F7 with the O2k connected The O2k control key F7 provides fast access to the O2k control window Load setup After selecting a setup it must be L l...

Страница 15: ...ibed above with the next sequential number After re connecting the O2k continues operation uninterrupted the experimental time is reset to zero and data recording is restarted with automatic saving to...

Страница 16: ......

Страница 17: ...t O2k Chamber Stirrer Bar white PVDF 15x6 mm OroboPOS Holder O2k Chamber Holder A2_Insert OroborPOS OroboPOS Connector A3_O2k Chamber volume calibration Volume Calibration Ring Stopper black PEEK coni...

Страница 18: ...chamber into the copper block of the O2k FluoRespirometer 2 Screw the POS holder 1 into a position where the glass chamber with the angular cut towards the POS holder is slightly lifted upwards 3 Scre...

Страница 19: ...of the stopper black PEEK is not part of the effective chamber volume When titrating 2 1 ml insert the tip of the pipette to the wall of the glass chamber to add the entire volume Liquid must not be l...

Страница 20: ...the manual Trouble shooting of test versions DatLab 7 1Andras M sz ros Oroboros Instruments AM wrote and edited the manual Taking editing photos and images 1Ondrej Capek Oroboros Instruments OC was m...

Отзывы: