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11

3.5 THEORY OF OPERATION

The rotor shaft is coupled directly to the drive motor, via the drive pin. 

When attached to the homogenizer motor, the rotor shaft can spin 

up to 35,000rpm. This assembly makes up the rotor portion of the 

rotor-stator generator probe. The tube/collar assembly is attached to 

the motor housing, but does not spin. This is the stator portion of the 

rotor-stator generator probe. As the rotor knife spins within the tube 

and collar assembly, it creates a pumping action, pulling the sample 

into the open end of the generator probe and forcing the sample out 

through the windows in the tube. The interaction of the rotor knife 

with these windows sets up a shearing action, reducing the particle 

size of the sample. The speed differential between the rapidly moving 

portion and the relatively stationary portion of the sample sets up a 

second force called cavitation which pulls the sample apart, further 

reducing the particle size.

The processing efficiency can be affected by:

•  Amount of material processed vs. size and speed of the  

generator probe. 

•  Container geometry and size (round vessels encourage  

swirling, while fluted or cornered vessels disrupt flow patterns 

for more effective mixing/processing).

•  Processing speed vs. optimal speed.

•  Size and type of material and flow characteristics (material  

particles must be small enough to be carried into the    

generator head for optimal processing).

SECTION 3 — OMNI TH OPERATION

Содержание PCR-258

Страница 1: ...REV B PCR 258 OMNI Tip HOMOGENIZING KITS USER MANUAL...

Страница 2: ...ithout charge For the balance of the period parts will be provided but service will be charged at established labor rates This warranty does not include normal wear from use it does not apply to any i...

Страница 3: ...ion fire or injury Use this product only for its intended purpose as described in this booklet DO NOT use attachments not recommended by the manufacturer DO NOT operate the product if it is damaged in...

Страница 4: ...for those applications WARNING Keep all housings in place and in working order WARNING Remove all tools from the generator probe before turning the motor on WARNING DO NOT use the motor in a dangerou...

Страница 5: ...he Omni TH 3 1 Stand Mounting the Omni TH 9 3 2 Installing Rotor Stator Generator Probes 9 3 3 Omni Tip Plastic Probes 10 3 4 Omni TH Operation 10 3 5 Theory of Operation 11 Section 4 Operational Guid...

Страница 6: ...n for more information Omni s Tissue Homogenizing Kits are designed for situations where molecular contamination between samples cannot be tolerated Stainless steel generator probes are available for...

Страница 7: ...ssue Homogenizing Kits is required for mounting Omni Tips to the Omni TH Homogenizer and is not disposable See Section 5 for more information about Omni Tips Part Number A1000SB 12 120 30750 30750H 32...

Страница 8: ...03 100 mL Height motor only 22 9 cm 9 in Weight motor only 466 g 16 4 oz Electrical Requirements 115 V 60 Hz or 220 V 50 Hz Standards Approval Compliance 220V CE Certified Description Motor Drive Unit...

Страница 9: ...n firmly The unit will lock in place 4 To remove the TH press the black button on the back of the opening of the post clamp assembly and lift the motor from the cradle 3 2 INSTALLING ROTOR STATOR GENE...

Страница 10: ...he sample vessel can significantly affect flow patterns within the vessel This also affects processing efficiency As a rule of thumb the generator probe usually operates most efficiently at a depth of...

Страница 11: ...eraction of the rotor knife with these windows sets up a shearing action reducing the particle size of the sample The speed differential between the rapidly moving portion and the relatively stationar...

Страница 12: ...n times for repeated use they are not recommended for extended use with phenol or guanidine based reagents see Section 5 3 for chemi cal resistance compatibility Disposal may be required when used in...

Страница 13: ...s Processing Speed Omni Tip Homogenizing Kits include a handheld Omni TH Homogenizer with motor speeds up to 35 000rpm Operating at the top speed enhances processing efficiency of the generator probes...

Страница 14: ...ntamination for many applica tions the probe can be cleaned sterilized or disinfected and reused by the procedures described below NOTE Omni Tip plastic probes are not factory presterilized or disinfe...

Страница 15: ...euse However phenol or guanidine based reagents commonly used in PCR studies are not recommended for extended use with Omni Tips Exposure to these chemicals shortens the life of Omni Tips and usually...

Страница 16: ...ft begins to warp as a result of autoclaving discard and lower the cycle time or temperature level accordingly for future runs 5 5 CLEANING THE OMNI TIP ADAPTER The Omni Tip 12 120 and A1000SB adapter...

Страница 17: ...he Omni TH is plugged in and turned on but is not functioning Check that the brushes are not worn and are correctly installed Replace if necessary see Section 3 2 Motor is turned ON and makes a makes...

Страница 18: ...18 NOTES...

Страница 19: ...19 NOTES...

Страница 20: ...www omni inc com OMNI Tip HOMOGENIZING KITS Omni International Inc 935C Cobb Place Blvd Kennesaw GA 30144 800 776 4431 770 421 0058...

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