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Last updated on Mar 2022 

BEGINNERS SAFETY MANUAL FOR  

OLYMPUS FV3000 CONFOCAL (#02-20) 

Produced by Shaalini, Graham Wright, Fiona Chia, Cris Barzaghi, Melvin  

1. Safety Guidelines ................................................................................................................... 2

 

1.1 Lasers ............................................................................................................................... 2

 

1.2 Immersion Oil ................................................................................................................... 2

 

1.3 Metal Halide Lamps (MHL) .............................................................................................. 2

 

2. Training .................................................................................................................................. 3

 

2.1 To arrange a training session: .......................................................................................... 3

 

3. Online Booking and System Access........................................................................................ 4

 

3.1 Acknowledgements .......................................................................................................... 5

 

4. Operation Procedures ............................................................................................................ 5

 

4.1 Switching ON Protocol ..................................................................................................... 5

 

4.2 Objective lens ................................................................................................................... 6

 

4.2 Software Initiation ........................................................................................................... 7

 

4.3 Setting up directory to save images ................................................................................ 8

 

4.4 Laser Configuration Settings ............................................................................................ 9

 

4.5 Using the LSM Imaging Tab ............................................................................................ 10

 

4.6 Image Adjustments 

–

 Setting the Gain and Offset properly.......................................... 11

 

4.7 How to do a Z stack for a 3D Image (xyz) ....................................................................... 11

 

4.8 For Time-lapse (xyzt) ...................................................................................................... 12

 

4.9 Reuse previous settings and export images .................................................................. 12

 

4.10 Switching OFF Protocol ................................................................................................ 13

 

5. Troubleshooting ................................................................................................................... 14

 

 

 

 

Содержание FV3000

Страница 1: ...oking and System Access 4 3 1 Acknowledgements 5 4 Operation Procedures 5 4 1 Switching ON Protocol 5 4 2 Objective lens 6 4 2 Software Initiation 7 4 3 Setting up directory to save images 8 4 4 Laser...

Страница 2: ...Immersion Oil Modern immersion oil has no known hazards to human beings so far yet they can cause discomfort to a person if the immersion oil has been left on skin for too long or inhaled Therefore pl...

Страница 3: ...taff before they are allowed to access the confocal system 2 1 To arrange a training session Please email Bioimaging Facility bioimaging tll org sg for a training session Fill in the necessary particu...

Страница 4: ...er 6pm Sat Sun Public holidays If users require more slots in a particular week they can book on weekdays during non peak hours after 6pm on weekends Sat and Sun and on public holidays If extra time i...

Страница 5: ...RT TIMING b Switch on the Master switch on the wall 1 PC 2 Wait until windows icon pops up c Switch on Stage controller box 3 CBH controller 4 d Optional Turn on the Metal Halide lamp 5 if needed e Tu...

Страница 6: ...t to 10x objective or lower then click YES Cleaning the stage will take some time b Otherwise click NO to skip cleaning 4 2 Objective lens Objective lens Immersion media 1 25x 0 04 Plan Apochromat Air...

Страница 7: ...n the stage mechanism This adds time to the initialization process Do not place anything on the stage mechanism until the initialization process is complete If you are not the first user of the week C...

Страница 8: ...y to save all your images Please save in your folder You have several different options on where you can save your images Option 1 D or E Users shaalini create your own folder BPAE cells oir Do keep i...

Страница 9: ...oduce intense background noise SD keep HV within 600 800 More than 800 will contribute to intense background noise Under PMT settings tab Mode Select VBF Average average a given number of scans to get...

Страница 10: ...to zoom images only in the center Ideal zoom which satisfy the Nyquist limit can be achieved by zooming till the red arrow any zoom beyond it will result in poor resolution and result in more photoble...

Страница 11: ...the laser power until red pixels disappear Increase Offset until a dusting of blue pixels in the background are observed Click LSM start to capture an image 4 7 How to do a Z stack for a 3D Image xyz...

Страница 12: ...your timelapse experiment Interval FreeRun continues to capture images until it has completed your selected cycle You could always choose an interval of your choice 4 9 Reuse previous settings and ex...

Страница 13: ...h the sample and return the stage to central position d Carefully clean the objective lens with Whatman lens tissue using 100 ethanol e Switch lens back to 10x 0 3 objective lens f Exit the FV31S SW s...

Страница 14: ...ission please contact Bioimaging Group No stage movement Check the coarse fine movement setting near the focus and xy position knobs It is a physical button that has no electronic indication elsewhere...

Страница 15: ...id white or coloured light sometimes red light might be indicative of error III Restart the main controller right at the bottom if none of the steps taken work especially if the machine has been left...

Страница 16: ...and cannot be recovered Restart computer terminal Startup popup error code LAST_OPERATION_ NONCOMPLETED The last user did not shut down the program properly or the program encountered a fatal error du...

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