Last updated on Mar 2022
3.1 Acknowledgements
If you use the TLL Microscopy and Imaging facility and/or have been trained or assisted any
of the bioimaging facility members in your research, then this should be acknowledged
appropriately in your publications and presentations.
4. Operation Procedures
Every confocal system in TLL Bioimaging Facility has its specific instructional manual which
are found in every confocal rooms. Strictly adhere to the correct order of operation for all
system. Failure to do so will result in disciplinary action from the facility. Any issues
encountered during the operation of the system are advised to seek help from the
Bioimaging facility.
Modification, exchange or removal of components beyond this operational manual is strictly
prohibited and is only carried out by the manufacturer, Bioimaging facility or by experienced
users approved by bioimaging facility. During operation of laser microscopy system, do not
look into the laser beam directly as they are all Class 3b and Class 4 lasers.
4.1 Switching ON Protocol
(a)
SIGN IN the Logbook and record your START-TIMING.
(b)
Switch on the Master switch on the wall
(1)
& PC
(2)
. Wait until windows icon pops
up.
(c)
Switch on Stage controller box
(3)
& CBH controller
(4)
.
(d)
(Optional)
Turn on the Metal Halide lamp
(5)
, if needed.
(e)
Turn on the Touch screen panel power button at the back
(6)
and wait for 5s to light
up.
(f)
Switching ON Lasers,
•
To use 405/488/561/640nm, switch on the power button on the main
combiner
(7)
, then turn the key to “ON”
(7A)
and finally turn on the
individual lasers that you need.
•
To use 445/514nm, switch on the power button on the sub combiner
(8),
then turn the key to “ON”
(8A)
and finally turn on the individual lasers that
you need.
(g)
(Optional)
Turn on the incubator
(9)
if required. It takes 10-15mins to stabilize.
(h)
Mount your slide onto the sample holder. If you need Live cell Imaging, turn on the
OkaLab incubator and the facility CO
2
(10)
. It takes 10mins to stabilize. If you require
help mounting the stage incubator holder onto the stage, please request help from
the TLL bioimaging department.
(i)
Log in with your TLL user account and d
ouble click on “FV31S
-
SW” to load the
software.
1st offence
A warning will be issued along with the reminder of the rules.
2nd offence
A second warning will be issued and your respective PI will be notified.
3rd offence
Banned from using any of the facility’s microscope for 2 weeks.
