Last updated on Mar 2022
•
Export Images:
➢
Right click on the image and
then export. An Export
windows will pop up.
➢
Select your directory where
you would like to save your
TIFF images into.
➢
Save as type: TIFF (*.tif, *.tiff
)
➢
Under Output format, select
RGB Color with merge and
24bit Full color.
➢
Click Save.
4.10 Switching OFF Protocol
Check through TLL microscopy resource booking if there is a user immediately after you. If
there is a user immediately after you, leave the system on and log off the PC. Otherwise
switch off the system.
(a)
Save your images through TLL common drive folder. Images saved in computer D/E
drives will be
deleted within a month without prior warning
.
(b)
Lower down the objective lens completely.
(Z value= 0)
(c)
Remove the specimen holder with the sample and return the stage to central
position.
(d)
Carefully clean the objective lens with Whatman lens tissue using 100% ethanol.
(e)
Switch lens back to 10x/0.3 objective lens.
(f)
Exit the FV31S-SW software and shut down PC
(2)
.
(g)
Press “OFF” on the touch screen panel and wait for 5 secs for the light to disappear.
Then, switch OFF the power button at the back of touch screen panel
(6)
.
(h)
Switch off the Stage controller box
(3)
, CBH controller
(4)
.
(i)
Switch off the HBO/Mercury lamp if used
(5)
. (Record down the lifespan hours in the
logbook.)
(j)
Switching OFF lasers,
•
To turn off 405/488/561/640nm, turn off the individual laser lines and turn the
key to “standby”,
(8)
. Switch off the main combiner
(7)
.
•
To turn off 445/514nm, turn off the individual laser lines and turn the key to
“standby”
(10)
. Switch off the sub combiner
(9)
.
(k)
Turn off the incubator
(12)
if it was on.
(l)
Finally, Switch off the master switch on the wall
(1)
.
(m)
Fill in the logbook with the objective lens used.
