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Olink Signature Q100, Руководство пользователя

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Olink Signature Q100 Скачать руководство пользователя страница 38

   

P1

P4

P2

P3

P5

Pressure Fluid

Actuation Fluid

Figure 15.  Control reagents pipetting map for the 24.192 chip

4.3.3  Run the 24.192 chip on Olink Signature Q100

NOTE:  

 

If you see the Lock icon, swipe it to unlock the system.

1.  If required, log in to the Olink Signature Q100 instrument.
2.  On the 

Start a New Run

 screen, tap 

Focus

.

NOTE:  

 

For more information about the task displayed on the screen, tap 

.

3.  On the 

Pipette samples, primers, and control reagents onto the chip

 screen, tap 

Open Drawer

.

38

Содержание Signature Q100

Страница 1: ...Olink Signature Q100 User Manual 1172 v1 1 2022 01 25...

Страница 2: ...s 28 4 1 Workflow Overview 28 4 2 Olink Target Workflows with the 96 96 and 48 48 chip 30 4 3 Olink Focus Workflow with the 24 192 chip 40 5 Troubleshooting 48 5 1 Run Instrument Checks 50 5 2 Export...

Страница 3: ...alerts for chemicals Indicator Description Pictogram see example consisting of a symbol on a white background within a red diamond shaped frame Refer to the individual safety data sheet SDS for the ap...

Страница 4: ...from Olink either alone or as part of this kit go to olink com and search for the SDS using either the product name or the part number Some chemicals referred to in this user guide may not have been...

Страница 5: ...ets of primers This innovative solution for real time PCR provides experiment densities far beyond what is possible with microplate platforms significantly reducing the number of liquid handling steps...

Страница 6: ...h the Olink Signature Q100 system Component Label Color Supported chip Olink Signature Q100 Interface Plate 96 96 96010 Green Olink 96 96 chip for Protein Expression Olink Signature Q100 Interface Pla...

Страница 7: ...mm tubing into the compressed air inlet on the rear of the system and regulate the incoming air to 80 90 psi or 5 5 6 2 bar of CDA POWERED BY FLUIDIGM LAN AC 100 240V 50 60Hz 8A USB USB HDMI COMPRESS...

Страница 8: ...be reached Damage control and limitation in case of an incident via the smaller attack surface A smaller subset of network devices is within the same scope Improved access control for external and int...

Страница 9: ...networks can be established if the scenario requires it 2 8 Regulatory Compliance The following directives and harmonized standards were used to evaluate the safety and performance of the Olink Signa...

Страница 10: ...ink Signature Q100 System Software 3 1 1 Unlock the Touchscreen Swipe toward the unlock icon to unlock the touchscreen 3 1 2 Log In to the Olink Signature Q100 System If required enter your username a...

Страница 11: ...passwords To access the User Accounts settings you must have an account that has Manager permissions see section 3 2 User Accounts Instrument Check Run instrument checks on the Olink Signature Q100 in...

Страница 12: ...ructions for how to perform the tasks required for that step of the workflow To close the on screen help window tap in the top right corner 3 1 5 View Run Logs and Export Data Tap History to view run...

Страница 13: ...port The files are copied to the USB drive A Success message is displayed when the export is complete If there was a problem with the run Technical Support might request the raw data files for that ru...

Страница 14: ...o export 3 Tap Export The files are copied to the USB drive A Success message is displayed when the export is complete 3 1 6 Delete Run Files After you export run files you can delete them from the in...

Страница 15: ...exported run files In the History screen exported runs show a checkmark in the right column NOTE Run files must be exported before they are deleted 1 Go to the History screen 2 Tap or in the Export c...

Страница 16: ...3 1 8 Change System Time Settings 1 Tap Settings System then tap Edit next to Time Settings 2 Change the time zone and the date and time then tap OK 16...

Страница 17: ...3 To view the time in 24 hour format select the 24 Hour Format checkbox 4 Tap Save Changes Tap Yes to update the system settings If you do not want to keep your changes tap Cancel 17...

Страница 18: ...e system settings Tap Cancel if you do not want to keep your changes For information about running instrument checks manually see 5 1 Run Instrument Checks 3 1 10 Change the Screen Lock Timeout 1 Tap...

Страница 19: ...the Require Authentication checkbox then tap Save Changes 4 Tap Yes to update the system settings If you do not want to keep your changes tap Cancel After enabling authentication you can access the U...

Страница 20: ...t to request a recovery key The Olink Signature Q100 system has three types of user accounts Account Type Description Manager Managers can Log into the system Start new runs and abort runs started by...

Страница 21: ...or Operator 5 Tap Save 3 2 2 Disable a User Account A user with a Disabled account is no longer allowed to log in but the account is still in the system for auditing purposes IMPORTANT To disable a us...

Страница 22: ...tails screen 4 Tap 5 Tap Yes sure to delete the user account If you do not want to delete the user account tap No 3 2 4 Change Your Password When Authentication is enabled a user with an Operator acco...

Страница 23: ...g particulates reagents and fluids to the chip surface 3 3 2 Bubble Prevention Vortex the 96 well primer and sample plates gently low speed but thoroughly at least 5 sec to allow all reagents to mix h...

Страница 24: ...epare the chip on Signature Q100 This primes the chip ready for use Remove the chip from the instrument and pipet each final sample and primer mix into the chip 3 Run the chip and collect data on Olin...

Страница 25: ...uid Do not evacuate air from a syringe before injecting control line fluid Step 4 Optional step to ensure proper chip function 2 Actuate the check valves a Place the chip on a flat surface b Use the s...

Страница 26: ...proximately 5 sec before withdrawing the syringe Before removing the syringe from the accumulator ensure that all the control line fluid and air are purged from the syringe This is to avoid dripping f...

Страница 27: ...displayed on the screen tap 3 After you tap Target 96 or Target 48 the drawer opens 4 Place the chip containing control line fluid and the interface plate on the drawer Align the notched corner of th...

Страница 28: ...eturn to the Insert a chip with control line fluid into the instrument screen 6 Tap Start Preparation begins and displays the time remaining NOTE If you need to abort the preparation tap Abort at the...

Страница 29: ...p Eject in the bottom right corner to open the drawer 8 Remove the chip and interface plate from the instrument then tap Close Drawer IMPORTANT Do not leave the drawer open while you are pipetting sam...

Страница 30: ...orientation of the A1 corner The barcoded edge is on the left side NOTE Olink recommends using the reverse pipetting method when pipetting the sample and primer mixes See the Olink Target 96 User Manu...

Страница 31: ...If required login to the Olink Signature Q100 instrument 2 On the Pipette samples and primers onto the chip screen tap Open Drawer NOTE Olink Target 48 chip screen shown 3 Make sure that the Inteface...

Страница 32: ...the following details for the run Run Name the default name is the Chip ID Max number of characters is 100 Run Notes NOTE If you need to eject the chip tap Eject at the bottom of the screen The softw...

Страница 33: ...drive into the USB port on the front of the instrument b On the Run Successfully Completed screen tap Export Data to copy the run data to the USB drive NOTE You can export the data files later from th...

Страница 34: ...instrument 10 Tap Close Drawer 11 Return the interface plate to the storage container Discard the used chip 12 Analyze the exported data using the NPX Signature software NOTE You can export data onto...

Страница 35: ...ion 2 Actuate the top check valve a Place the chip on a flat surface b Use the syringe with the shipping cap to actuate the check valve in the top accumulator with gentle pressure Ensure that the popp...

Страница 36: ...chip surface Residual control line fluid or air in the pipette tip is normal 7 Make sure that the O ring returns to its normal position after the syringe is removed 8 Pull the protective film down and...

Страница 37: ...chip 5 Pipet 20 L of Pressure Fluid into each of the P4 and P5 inlets on the chip 6 Place the Olink Signature Q100 Interface Plate 24 192 over the chip aligning the barcoded edges 7 Use clear tape to...

Страница 38: ...k Signature Q100 NOTE If you see the Lock icon swipe it to unlock the system 1 If required log in to the Olink Signature Q100 instrument 2 On the Start a New Run screen tap Focus NOTE For more informa...

Страница 39: ...late and Chip Notched corner in chip and drawer 5 Tap Close Drawer The system scans the chip barcode then closes the drawer 6 On the Add run details screen confirm the Chip ID then enter any of the fo...

Страница 40: ...run begins and the screen displays the time remaining NOTE If you need to abort the run tap Abort at the bottom of the screen See 5 8 Abort a Run in Progress for more information 8 When the run is com...

Страница 41: ...n the Run Successfully Completed screen tap Export Data to copy the run data to the USB drive NOTE You can export the data files later in the History screen See 3 1 5 View Run Logs and Export Data for...

Страница 42: ...n the interface plate to the storage container Discard the used chip 13 Analyze the exported data using the NPX Signature software NOTE You can export data onto a USB drive or access it using ethernet...

Страница 43: ...uum Dust on the bottom of the chip or the top of the thermal chuck Confirm that the protective film was removed from the chip prior to loading on the tray Clean the thermal chuck using a lint free clo...

Страница 44: ...n Demonstration mode by mistake Contact Olink Technical Support to change the instrument back into Run mode Chip scan error Chip was loaded without its Interface plate on Repeat loading with correct I...

Страница 45: ...all components are connected and communicating properly The quick check takes 5 8 min to complete NOTE If a chip is located in the instrument it is not possible to execute the Run Instrument Checks R...

Страница 46: ...logs as follows 1 Insert a USB drive into the USB port on the front of the instrument 2 Tap select the date range for the logs you want to export then click Export The logs are exported to the USB dr...

Страница 47: ...USE T8AL 250VAC Main power switch Fuse holder blocks 3 Replace the fuse T8AL 250 VAC 5 20 mm and reinstall the fuse holder Follow the arrow marks in the fuse holder to match the casing 4 Plug the powe...

Страница 48: ...sure that the system components are working correctly Initialization and the instrument checks take approximately 10 min to complete Export the results from the Instrument Check screen under Settings...

Страница 49: ...h system shutdown NOTE If a run is in progress the screen displays a message NOTE You can either abort the run and shut down or wait until the run is complete before trying to power down the system ag...

Страница 50: ...l The system starts up and the instrument initializes all system components and software It also performs a set of instrument checks to ensure that the system components are working correctly The syst...

Страница 51: ...interface plate 3 Tap Close Drawer to return to the Home screen 5 9 Idle mode The instrument goes into idle mode automatically when there is no user interaction for a long while Touch the screen to t...

Страница 52: ...to cool down after a completed run AUTION C HOT SURFACE HAZARD The thermal cycler chuck gets hot and can burn your skin Use caution when working near the chuck 2 Tap Open Drawer 3 Remove the chip and...

Страница 53: ...ean the Instrument and Touchscreen Use a lint free cloth and 70 ethanol or 70 isopropyl alcohol to gently wipe the top of the instrument drawer and the instrument covers as needed Wipe down the touchs...

Страница 54: ...nd Biomedical Laboratories BMBL a publication from the Centers for Disease Control and Prevention and your lab s safety protocol to limit biohazard risks If biohazardous materials are used label the e...

Страница 55: ...inated surfaces with a wet cloth to remove residual alcohol and wipe dry 6 2 3 Disposal of Products Used chips and reagents should be handled and disposed of following federal state regional and local...

Страница 56: ...7 Technical support For technical support contact support olink com 56...

Страница 57: ...8 Revision history Version Date Description 1 1 2022 01 25 Added text to section 2 7 Editorial changes 1 0 2021 11 11 New 57...

Страница 58: ...related documents Title Document Number Olink Target 96 User Manual 0935 Olink Target 48 User Manual 1141 Olink Signature Q100 Site Requirements Guide 1170 Olink NPX Signature User Manual 1173 Olink S...

Страница 59: ...from the Centers for Disease Control and Prevention and your lab s safety protocol to limit biohazard risks If biohazardous materials are used label the equipment properly as a biohazard For more info...

Страница 60: ...wer and standby symbol Rear of instrument The Power switch is in the Off position Rear of instrument The Power switch is in the On position Inside of instrument for service personnel only Protective c...

Страница 61: ...ceable by the user ARNING W ELECTRICAL HAZARD Plug the system into a properly grounded receptacle with adequate current capacity 10 4 Chemical Safety The responsible individuals must take the necessar...

Страница 62: ...dures All information in this publication is subject to change without notice Trademarks Olink and the Olink logo are trademarks or registered trademarks of Olink ProteomicsAB in the United States and...

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