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H301-PRIOR-NZ100-H117

User Manual

REV 02

www.oko-lab.com

Pa

ge

2

Index

Components and dimensions................................................................................................... 3

Insertion of the Sample Feedback Temperature Sensor .................................................................. 4

Working with 35 Petri Dish- .................................................................................................... 4

Working with 1”x3’’ and 1”x2’’chamber slides

............................................................................ 5

Connection of the Gas Supply ................................................................................................. 6

Working with Perfusion ......................................................................................................... 6

Connection of the Chamber with Z piezo stage ............................................................................ 7

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Содержание H301-PRIOR-NZ100-H117

Страница 1: ...al REV 02 www oko lab com Page 1 H301 PRIOR NZ100 H117 Compatible with the following Z piezo stages Compatible with the following Okolab Controllers Prior Prior NZ100 NZ 200 in H117 H301 T BL PLUS UNO COMBINED CONTROLLER H401 T DUAL ...

Страница 2: ...1 Components and dimensions 3 2 Insertion of the Sample Feedback Temperature Sensor 4 3 Working with 35 Petri Dish 4 4 Working with 1 x3 and 1 x2 chamber slides 5 5 Connection of the Gas Supply 6 6 Working with Perfusion 6 7 Connection of the Chamber with Z piezo stage 7 ...

Страница 3: ...ctive coating and embedded temperature sensor Chamber riser It is a removable frame increasing the height of the chamber from 23 to 28 mm Distance between focal plane and upper side of the glass lid without the chamber riser Distance between focal plane and upper side of the glass lid with the chamber riser Chamber top view Lid Chamber Riser Chamber Main Body Sample Holder Order Separately Figure ...

Страница 4: ...les when not in use Grub screws M3x6 Remove small screw as necessary before introducing the sample Feedback Tem perature Sensor 1 Frontal view 2 3D view Temperature Sensor dedicated opening Temperature Sensor dedicated opening Figure 2 Insertion of the temperature sensor inside the chamber 3 Working with 35 Petri Dish The closing parts A in Image1 of Figure 3 and the flat springs B in Image 3 of F...

Страница 5: ...orking with 1 x3 and 1 x2 chamber slides Flat springs prevent movement of 1 x3 and 1 x2 chamber slides inside of the sample holder A A 1 2 Figure 4 Sample holder for1 x3 chambered cover glass holder 2 1 A A Figure 5 1 Sample holder for 1 x2 chambered cover glass holder ...

Страница 6: ...gently pushing silicon tubing onto brass opening Gas Input Figure 6 Connection with gas supply 6 Working with Perfusion The Chamber Riser included with H301 PRIOR NZ100 H117 features 12 perfusion holes for the insertion of perfusion tubing up to 2 5 mm in outer diameter Small screws plug the perfusion holes when not in use Grub screws M3x6 Remove small screws as necessary before introducing perfus...

Страница 7: ...ct the chamber with Z piezo stage 1 Place the sample holder on the stage and tighten the four M2x3 Pan head screws as shown in Figure 8 Image 1 2 Place the chamber main body on the stage and tighten the two M2x3 Pan head screws as shown in Image 2 and 3 Figure 8 3 Place the lid on the chamber as shown in Image 3 of Figure 8 1 2 4 3 Screw M2x3 Screw M2x3 Figure 8 How to assemble the chamber to the ...

Страница 8: ...e chamber cools down To clean the body and the glass lid of the chamber wipe with a soft micro fiber cloth For stubborn smudges you can damp the soft micro fiber cloth with ethyl alcohol product code UN1170 Do not put any liquid di rectly on the chamber While cleaning the glass lid do not apply strong force to the surface of the glass lid because it can be damaged H301 PRIOR NZ100 H117_User Manual...

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