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H301-MINI

User Manual

Rev 02

www.oko-lab.com

Connection of the Gas Supply

A single silicon tubing carries output gas from the Okolab Gas Controller to the H301-MINI. Silicon tubing connects to a

gas input - brass opening - located on a corner of the H301-MINI. See Figure 11. Connect by gently pushing silicon tub-

ing onto brass opening.

Gas Input

Figure 11.Connection with gas supply.

Working with Perfusion

The Chamber H301-MINI features 12 perfusion holes on the sides for the insertion of perfusion tubing up to 2.5 mm in

outer diameter. Small screws plug the perfusion holes when not in use. (

screws plug M3x0.5 THD

). Remove small

screws plug as necessary before introducing perfusion tubing.

Figure 12 shows location of perfusion holes.

1.Frontal view

2.3D view

Perfusion dedicated opening

Figure 12. Perfusion

Содержание H301-MINI

Страница 1: ...age 1 H301 MINI Compatible with the following XY stages Compatible with the following Okolab Controllers Physik Instrumente P 737 1SL 2SL 5SL Ludl 99A600 It fits in any MW plate holder H301 T BL PLUS...

Страница 2: ...rtion of the Sample Feedback Temperature Sensor 4 4 Insertion of Sample Holder into Chamber 5 5 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic Locks 5 6 Working with 1x3 and 1x2 chambe...

Страница 3: ...nts Chamber main body with embedded temperature sensor Heated glass lid with Indium Tin Oxide ITO conductive coating and embedded temperature sensor Distance between focal plane and upper side of the...

Страница 4: ...M single 1 60mm Petri dish 1x35 M single For 1 35mm Petri dish holder 1x60 M single For 1 60mm Petri dish holder 1xGS M single For 1 1 x3 chamber slide holders 1xLABTEK M single For 1 1 x2 Labtek cha...

Страница 5: ...or 60 mm Petri Dish Spacing Rings and Magnetic Locks Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder Figure 5 illustrates the available magnetic locks for 35 and 60 mm...

Страница 6: ...ck NOTE Spacing rings are included with sample holder Grainer Petri35x10 Ibidi Dish35mm low Corning 35x10mm MatTek P35G X 14 X Willco 35x10 BD Falcon 35x10 Figure 7 Ring selection scheme for 35 mm dis...

Страница 7: ...etic locks prevent movement of 1 x 3 and 1 x 2 chamber slides inside of the sample holder NOTE Magnetic locks are included with sample holder 1 2 Figure 9 Magnetic lock for 1 x 3 chamber slide 1 3 2 F...

Страница 8: ...ilicon tub ing onto brass opening Gas Input Figure 11 Connection with gas supply 8 Working with Perfusion The Chamber H301 MINI features 12 perfusion holes on the sides for the insertion of perfusion...

Страница 9: ...ber on the stage follow the steps shown in Figure 13 1 Place the chamber on the stage See Image 1 of Figure 13 2 Incline the chamber and slide it in direction of the springs until it snaps into the ho...

Страница 10: ...e chamber cools down To clean the body and the glass lid of the chamber wipe with a soft micro fiber cloth For stubborn smudges you can damp the soft micro fiber cloth with ethyl alcohol product code...