NuAire NU-5500, Руководство по эксплуатации и обслуживанию

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OM0133 16
8.0 DH Autoflow Operation
CAUTION: All maintenance actions on this equipment must be performed by a qualified
technician who is familiar with the proper maintenance procedures required for this
equipment, as well as repair.
The incubator is designed to provide a sterile, constant temperature, constant CO
2
level and naturally
humidified atmosphere for optimum growth of tissue cell cultures and other organisms requiring this
precise environment. To operate the incubator properly, the following parameters must be reviewed,
carefully set, and/or prepared.
8.1 Sterility
The environment provided by this incubator is not selective. As a result, any contamination within the
chamber is subjected to the same environment as the specimens. Therefore, before placing any cultures in
the incubator, the shelves and sidewall top plenums should be sterilized. The interior sidewalls, top,
bottom, door, as well as the gasket should be wiped clean with a 70% solution of isopropyl alcohol or
other disinfectant, to remove any contamination. Use mild detergent to clean the exterior of the
incubator. The plastic fan wheel cannot be easily cleaned. It is recommended that this wheel be removed
and replaced each time the incubator is sterilized.
8.2 Humidity
Humidification of the incubator chamber is achieved through the process of water evaporation vapor
water pressure from a stainless steel water pan placed on the bottom of the chamber. Materials of
different thermal resistance i.e. glass, plastic do not offer sufficient thermal recovery and are not
recommended for use. Although some metals offer better thermal coefficients than stainless steel,
dissimilar metals cause electrolysis in the acid atmosphere carbonic acid and should never be used, or
placed within the chamber.
Use only distilled or mineral-free water, no purer than 1 mega ohm, in the stainless steel pan. The water
should be changed at least once a week; preferably more often. FLOODING THE BOTTOM OF THE
INCUBATOR IS NOT RECOMMENDED since it is difficult to change the water weekly and almost
necessitates the use of chemicals, which are not recommended and may damage the stainless steel. Also,
it promotes condensation on the inner walls because it steals the natural convection, heat flow through the
inner chamber and condensation points occur.
ABSOLUTELY NO CHLORINATED OR HALOGEN
MATERIALS ARE TO BE USED IN THE CHAMBER .
Humidity recovery to 90% of original level within 20-40 minutes after a 15-second door opening with a
water reservoir area of 210 square inches. Contamination in the water pan may be avoided by adding a
small amount of copper sulfate to the water pan after every decontamination of the chamber.
Condensation on the glass door, walls, top, or bottom of the chamber indicates an incorrect balance of
door front perimeter heat. Both the door and front perimeter heaters operate on a duty cycle. A good
starting point for these duty cycles is the default setting for the door & perimeter heaters in a room
ambient temp of 22ºC 72ºF at a temperature set point of 37.0ºC. The fresh airflow that the air pump
delivers to the chamber has been preset at the factory. If condensation starts forming on the sides or
backwall in the chamber, the number and length of air injections into the chamber can be increased. (See
section 8.8.1.2 for instructions). To alleviate the condensation, increase the airflow. To increase RH in
the chamber, reduce the airflow. If airflow is decreased, some condensation may be unavoidable. The
plastic plug with breather holes must be used on the side port.
DO NOT SEAL THE SIDE PORT
OPENING.
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ATTENTION ACCOMPANY'S
INFORMATION OR IMPORTANT
SYMBOL POTENTIAL ELECTRICAL
HAZARD ONLY QUALIFIED
PERSON TO ACCESS
188
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