Manual: TIRF Microscope
Ee-1454
Martijn de Gruiter
Erasmus OIC
5
Metamorph - Taskbar
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On startup your personal taskbar is loaded
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Metamorph functions can be accessed easily from a
Taskbar and an
Acquire window
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You can restore illumination settings with the
button: "Restore OIC settings"
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Open the Acquire window with Acquire
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Access Acquisition modes with: MDA & Stream
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Select preferred illumination settings
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Left side: lasers for camera,
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right side: mercury lamp or transmission to
eyepiece
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Open FRAP or laser console
Metamorph - Acquire window
Sidebar left:
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Exposure time
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Minimum interval time at full chip is 90ms, quad chip is 50ms
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Exposure time can be lower than interval time
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Full Chip, Quad Chip, Use Active Region
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Use full chip, middle quadrant, or region selected by ROI
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Live bin & bin
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Combine pixels to increase readout speed
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Use only for live viewing or real recording
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Normal state = 1
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Start Live/Stop Live
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Start/stop live mode
Acquire images
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To acquire live mode press "Acquire" button
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Separate images can be recorded as separate images or in stack
