Manual: TIRF Microscope
Ee-1454
Martijn de Gruiter
Erasmus OIC
3
Start system
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Turn on the power by the power socket (on the floor under the table)
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Switch the mercury lamp on and then ignite it.
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If not needed don’t turn the mercury lamp on
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The unit on the shelf closest to the door
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Turn on all devices with a button on the shelf and the table.
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Camera and laser box are automatically enabled
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Next turn on the device with a key: Laser control panel
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Start the computer and start the program Metamorph.
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Select suited user account.
Live Cell imaging:
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When imaging at 37
o
C:
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The heating device to maintain your cells uses water.
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fill reservoir with demineralized water up to level of CO2 tube
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Allow the system to warm up for 15-30 minutes
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Settings for the heating unit are paper on the wall, choose your settings
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General settings for cells on a coverslip:
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top heater 40.5 ° C
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bath heater 38.5 ° C
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stage heater 38.5 ° C
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lens heater 37 ° C
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Ensure that the cells are grown on a glass coverslip
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Place the slide in the metal ring, tighten firmly but not too hard because it will break the
glass
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Add 1-2 ml of the medium from the appropriate well. There is a convex surface
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Move with a paper towel around the edge of the coverslip from below with a little pressure,
if no medium leaks from the ring your seal is good.
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Wipe clean the underside of the slide with ethanol to prevent contamination of the
objective and microscope