Nikon 50i, Инструкция по эксплуатации

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Chapter 2 Microscopy
2.3 Microscopy with Epi-fl Attachment Mounted
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2
2.3
Microscopy with Epi-fl Attachment Mounted
Before microscopy
• Check the cumulative operating hours of the mercury lamp. Replace the lamp if
its cumulative operating hours exceed the average service life.
• Use non-fluorescent slide glass.
• Use non-fluorescent immersion oil.
• To keep the specimen color from fading, keep the shutter closed when not
performing microscopy.
1
Perform steps 1 through 10 in “2.1 Bright-Field Microscopy.”
2
Turn off the microscope power
switch.
3
Close the shutter and block the
light emitted by the mercury lamp.
⇒ P.51
4
Insert the desired excitation filter
cube into the optical path.
⇒ P.52
Close
the shutter.
Select a cube using the filter
cube switching knob.
Press the power switch
to the “
◯
” position.