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Chapter

Microscopy

2.1

Bright-Field

Microscopy

Adjust the diopter and the
interpupillary distance.

⇒

P.43

⇒

P.44

Focus and center the condenser.

⇒

P.45

Switch to the desired objective
and view the specimen.

Adjust the field diaphragm and
aperture diaphragm each time you
change objectives.

⇒

P.46

⇒

P.47

Aperture diaphragm

knob

Field diaphragm

knob

Select the

desired

objective.

Center the condenser

using the condenser

centering screws.

Focus the condenser

using the condenser

focus knob.

Содержание 50i

Страница 1: ... Established 1981 Advanced Test Equipment Rentals Advanced Test Equipment Rentals www atecorp com 800 404 ATEC 2832 ...

Страница 2: ...Microscope ECLIPSE 50i ECLIPSE 55i Instructions M317E 04 2 CF 2 1 2 ...

Страница 3: ......

Страница 4: ...ucts attached to or to be used with this product super high pressure mercury lamp power supply high intensity light source etc Warning Caution symbols used in this manual Although Nikon products are designed to provide the utmost safety ignoring safety precautions or improper use may result in personal injury or property damage as well as voiding the terms of the warranty To ensure safe use please...

Страница 5: ... symbol found on the lamphouse of the ECLIPSE 50i indicates the following The lamp and surrounding areas including the lamphouse become very hot during and immediately after a period of illumination Risk of burns Do not touch the lamp or surrounding areas during or immediately after a period of illumination Make sure the lamp and surrounding areas have cooled sufficiently before attempting to repl...

Страница 6: ...Make sure you have read the instruction manual for the light source super high pressure mercury lamp power supply or high intensity light source 4 Power cord for ECLIPSE 50i and the power cord for AC adapter Use one of the power cords specified Using the wrong power cord may result in fire or other hazards The product is classified as subject to Class I protection against electrical shock Make sur...

Страница 7: ... is scratched fouled subjected to high external pressure or physical impact or used beyond its service life the sealed gas may escape or the lamp may burst resulting in gas inhalation injury from glass or other injury 3 When the lamp is lit the lamp and surroundings will become extremely hot Touching the lamp with bare hands may result in burns flammable materials placed near the lamp may ignite 4...

Страница 8: ... is open Do not break up used lamps instead dispose of them as special industrial waste or as specified by local regulations 3 Use the specified lamp when using the ECLIPSE 50i The product s built in power source is used for the halogen lamp that is a light source for the diascopic illumination A halogen lamp up to 6V 30W can be lit Always use the specified halogen lamp Using an unspecified lamp m...

Страница 9: ... imparing performance or generating malfunction Operating conditions temperature 0 to 40 C humidity 85 RH max no condensation Transporting storage conditions temperature 20 to 60 C humidity 90 RH max no condensation 8 Use the product with the wiring cover when using the ECLIPSE 55i Do not remove the wiring cover except when assembling the product or replacing batteries Using the system without the...

Страница 10: ...llic objects such as necklaces or hairpins The battery may become hot leak or burst Do not expose to direct sunlight or leave inside a sun heated car with all windows shut The battery may become hot leak or burst Do not drop or expose to strong impact The battery may become hot leak or burst Keep out of reach of small children The battery can be swallowed by small children If swallowed consult a p...

Страница 11: ... result in malfunctions or poor performance Consider the following factors when selecting an installation location Select a vibration free location Install the product on a level surface Install the product at least 10 cm away from walls Choose a location less exposed to hazards in the event of collisions earthquakes or other potential disasters To keep the product from falling use strong rope or ...

Страница 12: ...t degrade over time Replace them after the appropriate number of hours Filter characteristics may alter if the filter is exposed to high humidity To prevent changes in or degradation of filter characteristics avoid using or storing the filters under conditions of high humidity or high temperature Avoid subjecting filters to rapid temperature changes When a filter is not in use store in a desiccato...

Страница 13: ...Riser Eye Level Riser C TE Ergonomic Binocular Tube Ergonomic Binocular Tube C TEP DSC Port for Ergonomic Binocular Tube DSC Port J FL 50i55i Epi Fluorescence Attachment Epi fl Attachment J CY Cytodiagnostic Unit Cytodiagnostic Unit C HS Hand switch Hand Switch DS Camera Head DS 5M Camera Head DS Camera Control Unit DS L1 DS L1 DS Camera Cable Camera Cable Super High Pressure Mercury Lamp Power Su...

Страница 14: ...idual Operations 34 3 1 Power ON OFF 35 3 1 1 Microscope 35 3 1 2 Cytodiagnostic unit 35 3 1 3 Light source of the Epi fl attachment mercury lamp 35 3 2 Brightness Adjustment 36 3 2 1 Adjustment using the brightness control knob 36 3 2 2 Adjustment using the preset switch 37 3 2 3 Adjustment with the ND filter IN OUT lever for 50i 37 3 2 4 Automatic adjustment after magnification change only for a...

Страница 15: ...g 50 3 15 2 Marking specimens 50 3 16 Fluorescence Observation 51 3 16 1 Warning 51 3 16 2 Shutter of the Epi fl attachment 51 3 16 3 Light shielding plate of the Epi fl attachment 51 3 16 4 Field diaphragm of the Epi fl attachment 51 3 16 5 Switching excitation methods 52 3 16 6 Inserting and removing the filter cubes 52 3 16 7 ND filters of the Epi fl attachment 53 3 17 Selecting Fluorescent Fil...

Страница 16: ... 6 1 Optical 74 6 2 Operational 75 6 3 Electrical 75 Chapter 7 Care and Maintenance 76 7 1 Lens Cleaning 76 7 2 Cleaning the Product 76 7 3 Disinfecting the Product 76 7 4 Storage 77 7 5 Periodic Inspections fee charged 77 Chapter 8 Technical Specifications 78 8 1 Specifications 78 ...

Страница 17: ...14 Part Names 1 1 1 Names of Main Components Eyepiece tube Eyepiece Microscope main body Objective Specimen holder Condenser Stage Revolving nosepiece ...

Страница 18: ...justments 1 2 1 Right view Optical path switching lever Diopter adjustment ring Refocusing lever Aperture diaphragm knob X stage knob Y stage knob Brightness control knob Field diaphragm knob Preset switch Preset brightness control knob 50i ND filter IN OUT lever 55i Color compensating filter IN OUT lever ...

Страница 19: ...r 1 Part Names 1 2 Names of Parts Used to Make Adjustments 16 1 2 2 Left view Power switch Condenser focus knob Condenser centering screws Fine focus knob Coarse focus knob Coarse focus torque adjustment knob ...

Страница 20: ...Names 1 2 Names of Parts Used to Make Adjustments 17 1 1 2 3 Rear view 50i 50i with lamphouse cover open Tool Handle Wiring guides Wiring guides Lamphouse Halogen lamp AC inlet Input voltage indication Lamphouse cover ...

Страница 21: ...Handle Wire guides Wiring cover Connector J4 for cytodiagnostic unit signal cable Connector J2 for cytodiagnostic unit power supply cable Connector J3 for cytodiagnostic unit hand switch Connector J1 for AC adapter Battery holder Cytodiagnostic unit signal cable Cable for cytodiagnostic unit hand switch Cytodiagnostic unit power supply cable AC adapter cable ...

Страница 22: ...Names of Parts Used to Make Adjustments 19 1 1 2 5 Ergonomic binocular tube with camera attached Camera centering screws Camera fine focus adjustment ring C mount Camera head Camera cable connector Attachment guide fixing screw ...

Страница 23: ...gnostic unit attached Side and rear views of cytodiagnostic unit Object marker knob Magnification indicator Hand switch 50i AC adapter cable thick 55i J CY PS Power supply cable thick 50i Hand switch cable thin 55i J CY SG Signal cable thin Cytodiagnostic unit power switch Ink cartridge ...

Страница 24: ... Fl attachment mounted Mercury lamp requires a separate mercury lamp power supply ND filters Filter cube replacement cover Field diaphragm lever Mercury lamphouse Filter cube motion restricting lever Shutter Light shielding plate Filter cube switching knob Optical path switching lever ...

Страница 25: ...e following section entitled 2 2 Microscopy with Cytodiagnostic Unit Attached 1 Turn on power P 35 2 Raise the condenser to the uppermost position 3 Fully open the field diaphragm and aperture diaphragm Raise the condenser using the condenser focus knob Fully open the aperture diaphragm using the aperture diaphragm knob Fully open the field diaphragm using the field diaphragm knob Press the power ...

Страница 26: ...en and move the portion to be viewed into the optical path P 42 6 Focus on the specimen P 40 Select the 10x objective Set a specimen and secure in place using the specimen holder Move the portion to be viewed into the optical path using the XY stage knobs Focus on the specimen using the coarse and fine focus knobs ...

Страница 27: ...denser P 45 9 Switch to the desired objective and view the specimen Adjust the field diaphragm and aperture diaphragm each time you change objectives P 46 P 47 Aperture diaphragm knob Field diaphragm knob Select the desired objective Center the condenser using the condenser centering screws Focus the condenser using the condenser focus knob ...

Страница 28: ...Chapter 2 Microscopy 2 1 Bright Field Microscopy 25 2 10Turn off power after completing observation Press the power switch to the position ...

Страница 29: ...the condenser to the uppermost position 3 Fully open the field diaphragm and aperture diaphragm Press the switch to the position Insert the cytodiagnostic unit power switch Raise the condenser using the condenser focus knob Fully open the aperture diaphragm using the aperture diaphragm knob Fully open the field diaphragm using the field diaphragm knob ...

Страница 30: ...he diopter and interpupillary distance P 43 P 44 7 Focus and center the condenser P 45 Set a specimen and secure in place using the specimen holder Move the portion to be viewed into the optical path using the XY stage knobs Focus on the specimen using the coarse and fine focus knobs Center the condenser using the condenser centering screws Focus the condenser using the condenser focus knob ...

Страница 31: ...om the right toward the left then turn it toward the back to extend the marker 2 Gently press down the entire object marker knob to apply a mark 3 Turn the object marker knob toward the front to retract the marker P 50 4 Release both hands from the object marker knob 10Turn off all power switches after completing observations Select the magnification using the hand switch Press the cytodiagnostic ...

Страница 32: ...ent slide glass Use non fluorescent immersion oil To keep the specimen color from fading keep the shutter closed when not performing microscopy 1 Perform steps 1 through 10 in 2 1 Bright Field Microscopy 2 Turn off the microscope power switch 3 Close the shutter and block the light emitted by the mercury lamp P 51 4 Insert the desired excitation filter cube into the optical path P 52 Close the shu...

Страница 33: ...al for the light source 7 Set the 10x objective into the optical path 8 Set a specimen and move the portion to be viewed into the optical path P 42 9 Focus on the specimen P 40 Fully open the field diaphragm Select the 10x objective Set a specimen and secure in place using the specimen holder Move the portion to be viewed into the optical path using the XY stage knobs Focus on the specimen using t...

Страница 34: ...ecimen and the objective P 51 P 48 11To return to bright field microscopy Close the shutter of the Epi fl attachment and block the light emitted by the mercury lamp Turn on the microscope power switch to turn on the diascopic light source Turn the filter cube switching knob and move the position without a filter cube into the optical path 12Turn off all power switches after completing observations...

Страница 35: ...te adjustments tighten the screws firmly 2 Focus adjustment If the image viewed through the eyepiece appears to be in focus but the image on the monitor is out of focus turn the camera fine focus adjustment ring on the C mount until the image on the monitor is in focus Note that such out of focus situations can also indicate incorrect diopter adjustment Make sure you have made diopter adjustments ...

Страница 36: ...t photomicrography adjust white balance under normal lighting conditions before shooting 6 Capture and save images Position the specimen Refocus Adjust image brightness using the camera exposure compensation function Check the image using the Freeze button If the image is acceptable press the CAPT button to save the image The operating procedure differs if DF FL scene mode is selected For detailed...

Страница 37: ...llary Adjustment Eyepiece sleeve 3 8 Adjusting the Observation Position Ergonomic binocular tube 3 9 Adjusting the Condenser Position Condenser focus knob condenser centering screws 3 10 Adjusting the Aperture Diaphragm Condenser aperture diaphragm objective 3 11 Selecting a Condenser Condenser 3 12 Adjusting the Field Diaphragm Field diaphragm knob 3 13 Oil Immersion Operation Oil immersion objec...

Страница 38: ...starts initialization and sets the magnification of the cytodiagnostic unit to the 10x setting The 10x magnification indicator lights Press the power switch The switch pops out and the cytodiagnostic unit is switched off The magnification indicator turns off 3 1 3 Light source of the Epi fl attachment mercury lamp Refer to the operating manual provided with the mercury lamp power supply Always obs...

Страница 39: ...tachment 3 2 5 Epi fl image Erasure of transmitted image Microscope power switch 3 2 6 Monitor image Camera adjustment Application software for camera control Display mode exposure mode exposure compensation camera gain adjustment etc 3 2 7 3 2 1 Adjustment using the brightness control knob With the preset switch in the out position rotate the brightness control knob The brightness control knob is...

Страница 40: ...the actual image turn the knob with a precision screwdriver until the desired brightness is achieved Setting the preset switch to the depressed position enables the brightness level set with the preset brightness control knob For 55i when a cytodiagnostic unit is attached To adjust brightness automatically after switching magnification make sure the preset switch is in the out position 3 2 3 Adjus...

Страница 41: ...attach detach lever moves the ND filter into the optical path and darkens the fluorescent image ND filters are used to adjust light intensity Higher filter numbers correspond to lower transmission rates i e darker images ND filters do not affect color balance The table on P 53 shows the brightness levels achieved by different combinations of the three filters ND4 Reduces light intensity to 1 4 ND8...

Страница 42: ...hing lever allows distribution of light to the binocular section and camera port Optical path distribution Position of optical path switching lever Binocular section Camera port Pushed in 100 0 Ergonomic binocular tube Extended 50 50 Pushed in 100 0 Extended by one notch 20 80 Trinocular eyepiece tube T Extended by two notches 0 100 Pushed in 100 0 Trinocular eyepiece tube F Extended 0 100 3 3 2 D...

Страница 43: ...he coarse focus knob is located on either the right side or the left side A fine focus knob is provided on both sides To lower the stage Turn the knob toward the front To raise the stage Turn the knob toward the back 3 4 3 Number of knob turns and distance of stage travel No of knob turns Distance of stage travel vertical direction Coarse focus knob 1 turn Approx 13 8 mm Fine focus knob 1 turn App...

Страница 44: ...rn When turned in the direction opposite to arrow Makes knob easier to turn 3 4 5 How to refocus The entire stage can be lowered by setting a finger on the refocusing lever or top surface of the stage and pushing down When the finger is released the stage slowly returns to its original position If the stage is lowered to the lowest position it will be locked in that position Push down once again t...

Страница 45: ...along its vertical axis to the desired height 3 5 4 Adjusting the knob rotation torque When the X knob and Y knob are moved to the top and bottom positions the torque adjustment screws can be found between the knobs Turning the torque adjustment screw to move them closer towards the respective knobs increases rotational torque To increase rotational torque turn the adjustment screw counterclockwis...

Страница 46: ...oscopy to focus on the specimen with the 10x objective 3 Set the 40x objective in the optical path Using the coarse fine focus knobs focus on the specimen 4 Set the 4x or 10x objective in the optical path 5 Focus on the specimen using the diopter adjustment rings instead of the coarse fine focus knobs When making focus adjustments be sure to look through the right eyepiece with your right eye and ...

Страница 47: ...and focus on the specimen using the 10x objective Then move the eyepiece sleeve until the fields of view for the right and left eyes coincide 3 8 Adjusting the Observation Position The ergonomic binocular tube makes it possible to extend and tilt the binocular section Adjust the position of the binocular section for the most comfortable viewing Field of view Converge until the right and left field...

Страница 48: ...ce 4 Turn the condenser centering screws so that the field diaphragm image is positioned in the center of the field of view 5 Set the 40x objective in the optical path Turn the coarse fine focus knobs and focus on the specimen 6 Turn the condenser focus knob to form the field diaphragm image on the specimen surface 7 Adjust the condenser centering screws until the field diaphragm is at the center ...

Страница 49: ...m to less than 60 of the objective s numerical aperture 3 10 1 Adjusting the aperture diaphragm opening using the condenser scale Since the condenser scale indicates the numerical aperture adjust the aperture diaphragm ring according to the scale Normally the index on the aperture diaphragm ring should align with a scale line that corresponds to 70 to 80 of the numerical aperture of the objective ...

Страница 50: ...th an N A of 1 4 is used the maximum aperture of the swing out condenser or the Abbe condenser will be only about 65 of the objective s N A even when the condenser aperture diaphragm is wide open Refer to the condenser operating manual for information on phase contrast condenser 3 12 Adjusting the Field Diaphragm The field diaphragm controls the amount of illumination falling on the area of the sp...

Страница 51: ...ersed objective back and forth once or twice In the case of the condenser gently turn the condenser focus knob to move the condenser up and down slightly Add more oil Remove the oil and apply new oil Use as little oil as possible just enough to fill the space between the tip of the objective and the specimen or between the tip of the condenser and the specimen Too much oil will result in excess oi...

Страница 52: ...py procedures are the same as for oil immersion objectives Since water evaporates readily monitor the immersion water during observation Avoid using too much water since excess water will flow onto the stage and around the condenser promoting corrosion After use wipe off water from the tip of the objective and condenser then follow up by wiping with absolute alcohol If you observe water stains app...

Страница 53: ...sity does not vary if the brightness control knob is set to the maximum setting or if the preset switch is in the depressed position 3 15 2 Marking specimens Holding the object marker knob with both hands push it from the right toward the left then turn it toward the back to extend the marker Gently press down the entire object marker knob to apply a circular mark Turn the object marker knob towar...

Страница 54: ...t light 3 16 3 Light shielding plate of the Epi fl attachment The light shielding plate protects the observer s eyes from reflected ultraviolet light which is originally emitted through the objective from the specimen To remove the plate loosen the clamp screw and pull it forward 3 16 4 Field diaphragm of the Epi fl attachment The field diaphragm is used to restrict the illumination to the area of...

Страница 55: ...Lever position Filter cube switching Pulled all the way to the front Free switching possible Pushed in one increment notch Switching between positions 1 and 2 or between positions 3 and 4 The position at which the lever is pulled determines whether switching is for positions 1 and 2 or positions 3 and 4 Pushed in two increments notches Lock filter cubes cannot be switched 3 16 6 Inserting and remo...

Страница 56: ...ight intensity Higher filter numbers correspond to lower transmission rates i e darker images ND filters do not affect color balance ND4 Reduces light intensity to 1 4 ND8 Reduces light intensity to 1 8 ND16 Reduces light intensity to 1 16 As shown below you can combine these three filters to achieve various levels of brightness Brightness ND4 ND8 ND16 1 1 4 1 8 1 16 1 32 1 64 1 128 1 512 Outside ...

Страница 57: ... Different combinations of excitation and barrier filters may be selected for the same excitation method Other types of excitation filters barrier filters and dichroic mirrors can be purchased separately Excitation filters are exposed to strong light during operations and tend to age rapidly Replace the excitation filter at frequent intervals When using the UV2A or UV2B be sure to remove the space...

Страница 58: ...iking the specimen and causes the image to appear darker but reduces self fluorescence and fading For specimens with pronounced self fluorescence use excitation filters with a narrow bandwidth note that this will make the fluorescent image darker Excitation filters are exposed to strong light during operations and tend to age rapidly Replace the filter at intervals determined by usage Narrow EX fi...

Страница 59: ...hroic mirror an excitation filter and an LP filter type barrier filter will be incapable of exciting dyes that emit long wavelength fluorescent light for example TRITC in the case of FITC and TRITC This will result in very dark TRITC fluorescent images For such cases we recommend using multiband filters BP filter bandpass filter The bandpass filter passes only light of a certain wavelength blockin...

Страница 60: ...isted below cannot be inserted directly into the Epi fl attachment Instead as described below an internal spacer must first be removed or reversed 1 Place the filter cube on the work surface with the excitation filter facing up 2 Unscrew the ring retaining the excitation filter Be careful to avoid dropping the filter 3 Remove the spacer inside the ring 4 Remove or reverse the spacer as appropriate...

Страница 61: ...urate color reproduction is critical set the brightness control knob at a midpoint and use ND filters to make brightness adjustments Filter Place a commercially available color compensation filter on the filter holder at the microscope base as necessary 3 18 2 Adjusting the condenser Always focus and center the condenser Center the annular diaphragm for phase contrast microscopy For normal operati...

Страница 62: ...magnification with the objective rather than the zoom Generally the aperture of the objective increases with magnification The larger the numerical aperture the brighter the resulting image 2 Adjusting the excitation light Excessively bright excitation light will accelerate specimen fading while making it more difficult to acquire suitable fluorescent images Use ND filters to adjust brightness 3 S...

Страница 63: ...nearest Nikon representative to seek advice 2 Wiring at the Rear and Installing the Battery for the 55i 1 Confirm that the power switch of the microscope is turned off 2 Remove the rear wiring cover 3 Connect the appropriate cables 55i rear Connector J1 INPUT 12V DC C Connector J2 J CY PS Connector J3 SWITCH when using cytodiagnostic unit Connector J4 J CY SG Cytodiagnostic unit rear Connector A I...

Страница 64: ...ed battery when using the 55i on battery power Insert the battery into the battery holder If the 55i is running on battery power there is no need to connect the AC adapter to the J1 connector INPUT 12V DC at the rear of the 55i Always use the specified battery type EN EL1 Battery Battery holder CAUTION Cytodiagnostic unit connector 55i connector ...

Страница 65: ...the slot on the rear of the unit in the direction indicated by the arrow in the diagram The rear wiring cover should always be in position to protect the LED light source and battery holder from impact etc Lamp Lamphouse cover Slot for cover hook Lamphouse cover Cover hook CAUTION ...

Страница 66: ...he opening at the bottom of the arm For the 50i insert the AC adapter cable and the hand switch cable from the top of the arm and lead them out through the opening at the bottom of the arm 3 Secure the two cables in place using the guide at the back of the microscope as shown to the right 4 Replace the cover For the 50i AC adapter and hand switch cable For the 55i Signal cable thin J CY SG and pow...

Страница 67: ...lder on the stage and tighten screws 5 Attaching a Condenser 1 Turn the coarse focus handle until the elevating section is raised to the uppermost position 2 Turn the condenser focus knob until the substage is brought to the lowermost position 3 Insert a condenser and adjust so that it faces toward the front Secure in place with the tool stored in the back of the microscope 4 Turn the condenser fo...

Страница 68: ... as it will go 3 Insert the lamphouse into position 4 Turn the bayonet mount back to its original position to lock the lamphouse 5 Attach a mercury lamp 6 Connect the mercury lamphouse to the mercury lamp power supply 7 Attaching an Eyepiece Tube 1 Place an eyepiece tube on the microscope arm or Epi fl attachment 2 Secure in place with a clamp screw For the Epi fl attachment secure in place with a...

Страница 69: ...d with that of the fitting part 2 Secure in place with the tool stored in the back of the microscope 9 2 Attaching a Cytodiagnostic Unit 1 Insert the separately packed ink cartridge to the hole at the rear of the cytodiagnostic unit 2 Lift the cytodiagnostic unit from slightly forward of the point directly below the fitting part and slide toward the back to attach Continue sliding the cytodiagnost...

Страница 70: ...e binding band provided with the cytodiagnostic unit 10Attaching Objectives when a revolving nosepiece is attached Screw objectives into the revolving nosepiece When attaching the objective in this way make sure that the magnification of the objective increases when the revolving nosepiece is turned clockwise clockwise when viewed from above the eyepiece For the 50i AC adapter cable thick For the ...

Страница 71: ...l spacer must be removed or reversed as described on P 57 UV 2A UV 2B DAPI FITC GFP L GFP B TRITC Tx Red 3 Insert a nameplate into the position with the same address as the one indicated on the filter cube select knob on the right side of the microscope 4 Turn the filter cube select knob and insert a filter cube into the remaining open bay 5 Replace the cover Filter cube select knob Address indica...

Страница 72: ...lace with the tool stored in the back of the microscope 4 Attach the camera cable to the camera head Adjust the camera head position before using the camera Refer to 2 4 Photomicroscopy 12 2 When attaching to the trinocular eyepiece tube 1 Attach the camera head to the trinocular eyepiece tube using the DSC adapter 2 Attach the camera cable to the camera head Adjust the camera head position before...

Страница 73: ...the power cord to the AC adapter connecting to the cytodiagnostic unit Use only the specified AC adapter and power cord 3 Insert the other end of the power cord into a wall outlet 13 3 When not using the battery with the 55i 1 Check to confirm that the microscope power switch is off 2 Connect one end of the power cord to the AC adapter leading out from the wiring section on the back of the microsc...

Страница 74: ... bare lamp bulb with bare hands Soiling will reduce the service life of the lamp 1 Remove the lamphouse cover on the back of the microscope Remove the old lamp 2 Replace with a new lamp Avoid touching the bare lamp bulb with your bare hands Use only the lamp specified PHILIPS 5761 3 Replace the cover Engage the cover hook in the slot on the rear of the unit in the direction indicated by the arrow ...

Страница 75: ... humidity Avoid unusually cold or hot locations Recharge the battery at room temperature 5 C to 35 C To avoid degrading battery performance do not recharge the battery until it has been in use for some time and is at least partially discharged Battery temperature may increase somewhat immediately after recharging This does not indicate a problem and battery performance will not be affected If batt...

Страница 76: ...Avoid touching the object marker knob while the ink cartridge is removed 1 Remove the ink cartridge from the rear of the cytodiagnostic unit 2 Apply a few drops of ink to the red portion at the tip Overfilling may result in ink leakage 3 Once the ink is fully absorbed place a piece of paper over the tip to absorb any excess ink 4 Replace the ink cartridge Ink cartridge Ink cartridge Object marker ...

Страница 77: ...the field of view Field diaphragm image is not focused on the specimen surface Focus and center the condenser Dirt or dust is present on the lens or container Clean off the dirt and use a clean container Objective correction ring does not match the thickness of the container s bottom plate Adjust the correction ring Image quality is poor Contrast is poor Resolution is poor Field diaphragm image is...

Страница 78: ...ted or is connected improperly Connect it properly Lamp does not light Lamp has burned out Replace the lamp with a specified one Lamp burns out quickly Lamp type is incorrect Replace the lamp with a specified one Cables are not connected or are connected improperly Connect cables properly and push them in as far as possible see p 60 Power does not turn on even though the cytodiagnostic unit if use...

Страница 79: ...e or four times should be sufficient to clean the lens Never use petroleum benzine to clean the entrance lens at the bottom of the eyepiece tube or prism surface of the eyepiece tube Absolute alcohol and petroleum benzine are highly flammable Be careful when handling these materials particularly around open flames or when turning the power switch on or off Follow the instructions provided by the m...

Страница 80: ...e vinyl cover over the product to protect it from dust Switch off the microscope press the switch to the O position and wait for the lamphouse to cool before covering the product with the vinyl cover 7 5 Periodic Inspections fee charged To maintain the peak performance of the product we recommend periodic inspections Contact your nearest Nikon representative for more information Parts and service ...

Страница 81: ...ife 100 hours Input ratings 100 120V AC 230V AC 10 50 60Hz 0 9A 0 5A Power cord When used in 100 120V areas UL listed detachable power cord set 3 conductor grounding Type SVT AWG 18 3 m long maximum rated at 125V AC minimum When used in 220 240V areas EU EN approved 3 conductor power cord set 3 conductor grounding Type H05VV F 3 m long maximum rated at 250V AC minimum Operating conditions Temperat...

Страница 82: ...armful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense This Class A digital apparatus complies with Canadian ICES 003 Cet appareil numérique de la classe A est conforme à la norme NMB 003 du Canada Complies with Australian EMI AS NZS2...

Страница 83: ...r Manufacturer ILAN ELECTRONICS LTD Model F1650K Rated input voltage 100 240V AC 1 2A max when inputting 115V AC 50 60Hz Rated output voltage 12V DC 3 5A max Other UL listed product GS approved CE certified AC adapter power cord When used in 100 120V areas UL listed detachable power cord set 3 conductor grounding Type SVT AWG 18 3 m long maximum rated at 125V AC minimum When used in 220 240V areas...

Страница 84: ...requency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense This Class A digital apparatus complies with Canadian ICES 003 Cet appareil numé...

Страница 85: ...eld diaphragm Manual φ1 to 20 mm ND filter Manual 3 filters curved type filters ND4 ND8 ND16 Shutter Manual front operated Compatible lamp housing Hg Xe centered halogen incompatible with precentered type Operating conditions Temperature 0 to 40 C Humidity 85 RH max no condensation Altitude 2000 m max Degree of pollution Degree 2 Installation Category II Protection class Class I Indoor use only Tr...

Страница 86: ... Specified AC adapter Manufacturer ILAN ELECTRONICS LTD Model F1650K Rated input voltage 100 240V AC 1 2A max when inputting 115V AC 50 60Hz Rated output voltage 12V DC 3 5A max Other UL listed product GS approved CE certified AC adapter power cord When used in 100 120V areas UL listed detachable power cord set 3 conductor grounding Type SVT AWG 18 3 m long maximum rated at 125V AC minimum When us...

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