EVE™ PLUS, Automated Cell Counter
31
Troubleshooting
Trouble
shooting
Problem
Solution
Sample handling
•
Do not insert the EVE™ Cell counting slide upside-down
as this may introduce liquid into the instrument that could
damage it.
•
Do not reuse the EVE™ Cell counting slides, as leftover dye
from the previous reading may affect the next reading.
•
Do not use any other counting slides such as a glass
hemocytometer with the EVE™ PLUS as it results in inac-
curate cell count and may damage the instrument.
• En
sure that the sample covers the entire counting area
and the EVE™ Cell counting slide is inserted completely into
the counter.
Low and
high readings
•
The EVE™ PLUS is designed to read samples from 1 × 10
4
cells/ mL to 2 × 10
7
cells/mL, with the highest accuracy
between 1 × 10
5
cells/mL and 1 × 10
7
cells/mL.
•
If your sample is not in this range, you may need to dilute
the sample or add more cells and read the sample again.
Poor image quality
•
While viewing cells in the zoom mode, use the focus knob
to adjust the image to ensure that live cells have bright
centers, and dead cells have dark/blue centers.
Clumpy cell
• Ensure the cells are not clumped.
• To maintain instrument sensitivity, we recommend that
you calibrate the counter each year as described on page 12 .
Inaccurate cell count
