8
COMMISSIONING THE CABINET
Check all the following points before starting to commission the workstation.
1.
Switch "OFF" the cabinet at the Mains ELCB switch.
2.
The gas flow has been set at the factory to 35 litres per minute. However during initial
commissioning please check this gas flow as described on page 10 by placing a gas flow meter
and pressure gauge in the gas line.
3.
Open the mixed gas pressure regulator fitted to the gas bottle sufficiently high to maintain an inlet
pressure to the cabinet of approximately 30 psi (2 Bar) with the gas flowing.
4.
Ensure that the Gas Trap Bubble Bottle is filled up to the water level line and the gauntlets and
bungs are both fitted to the cabinet. (Page 7)
5.
Hang the Indicator Pump on the hook adjacent to the electrical socket, plug the pump into the
socket and switch "ON".
6.
Ensure that the bottom lid of the petri dish entry lock is in the closed position. Remove the top lid of
the lock and place it at an angle over the top of the lock ensuring that there is a gap for the flushing
gas to escape.
7.
Check that the Atmospheric Conditioning Sachet and the Palladium Conditioning Sachet are in
position underneath the shelf. The Atmospheric Conditioning Sachet should be put in first to the
rear of the cabinet
8.
Set the humidistat to 70% R.H.
9.
The commissioning switch will be found on the control panel. This is held in the 'OFF' position by a
small keeper. Slide the keeper and place the switch in the 'ON' position.
10.
Now switch on the cabinet at the E.L.C.B.
11.
Gas will begin to flow into the incubator and out of the lock. Adjust the gas flow if necessary (see
page 10)
12.
Leave the gas flowing for approximately 25 minutes then place the anaerobic indicator bottle in
position filled to the appropriate level with indicator solution and connect it to the pump with the
flexible tubing. (See the instructions regarding the preparation of the indicator solution on page 7)
13.
If the indicator solution remains colourless the cabinet is anaerobic. If the solution turns blue, leave
the gas flowing until it becomes colourless.
14.
During this period, the inside of the cabinet will mist over, but eventually this condensation will
clear. The catalyst sachet will also get quite warm.
15.
Once the cabinet is anaerobic, switch the commissioning switch off on the control panel and
replace the keeper to prevent accidental switching.
16.
Replace the lid of the petri dish entry lock.
17.
Now check all the functions are working.
