7
CHECKING THE INCUBATOR ATMOSPHERE FOR OXYGEN
The Anaerobic Indicator Solution will indicate traces of Oxygen in the incubator atmosphere by changing
colour from being clear when the atmosphere is anaerobic to being blue when oxygen is present. The
Indicator Solution is held in a small bottle located in the bottom of the incubator and is held in position by
a plastic holder. The atmosphere from the cabinet is bubbled through the solution by a small aquatic
pump. The pump is plugged permanently into an electrical socket. The atmosphere of the incubator can
be checked for oxygen content by simply switching the socket "ON" on the Control Panel. It is usually
sufficient to check the atmosphere perhaps once a day allowing the pump to run for about 2 minutes. If
the pump is allowed to run continuously, the indicator solution tends to evaporate and requires replacing
on a regular basis. This is not necessary.
PREPARING THE ANEROBIC INDICATOR SOLUTION
Make up the Anaerobic Indicator Solution from the two ingredients provided sufficient to fill the bottle
about two-thirds full using nine parts of solution "A" and one part of solution "B"
Now place a cap lightly on the neck of the bottle and place it in a bath of water and bring it to the boil. The
solution will change from being blue to colourless. Tighten up the cap and remove the bottle from the
water allow it to cool and pass it into the cabinet. The cap can now be removed and the outlet pipe from
the pump connected to the bottle. The bottle can now be placed in position inside the bottle holder where
it will be in full view.
We recommend replacing the solution on a weekly basis.
