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Appendix 

31 

60 electrode channels 

and accessories 

MEA recording system for 
inverted microscopes with 
advanced perfusion, 
60 electrode channels 

MEA60-Inv-2-
System-E 

 

Complete with 5 MEAs, data acquisition 
computer with MC_Card, 2 x MEA1060-Inv 
amplifier, MEAS2/1,  2 x TC02, 2 x PH01,  
power supply, and accessories 

MEA recording system for 
upright microscopes with 
advanced perfusion, 
60 electrode channels 

MEA60-Up-
System-E 

Complete with 5 MEAs, data acquisition 
computer with MC_Card, MEA1060-Up 
amplifier, TC02, PH01, power supply, and 
accessories 

MEA recording system for 
upright microscopes with 
advanced perfusion, 
60 electrode channels 

MEA60-Up-2-
System-E 

 

Complete with 5 MEAs, data acquisition 
computer with MC_Card, 2 x MEA1060-Up 
amplifier, 2 x TC02, 2 x PH01, power supply, 
and accessories 

MEA recording system for 
inverted microscopes, 
120 electrode channels 

MEA120-Inv-
System 

Complete with 5 MEAs, data acquisition 
computer with MC_Card, MEA1060-Inv 
amplifier, TC02, power supply, and accessories 

MEA recording system for 
inverted microscopes, 
120 electrode channels 

MEA120-Inv-4-
System 

 

Complete with 5 MEAs, data acquisition 
computer with MC_Card, 4 x MEA1060-Inv 
amplifier, MEAS4/2,  2 x TC02, power supply, 
and accessories 

MEA recording system for 
upright microscopes, 
120 electrode channels 

MEA120-Up-
System 

 

Complete with 5 MEAs, data acquisition 
computer with MC_Card, MEA1060-Up 
amplifier, TC02, power supply, and accessories 

MEA recording system for 
upright microscopes, 
120 electrode channels 

MEA120-Up-4-
System 

Complete with 5 MEAs, data acquisition 
computer with MC_Card, 4 x MEA1060-Up 
amplifier, MEAS4/2, 2 x TC02, power supply, 
and accessories 

 

Stimulus generators 

Product

 

Product 
Number

 

Description

 

2-Channel stimulus generator 

STG4002 

4-Channel stimulus generator 

STG4004 

8-Channel stimulus generator 

STG4008 

General-purpose stimulus generators for current and 
voltage-driven electrical stimulation, 4000 series, 
with integrated stimulus isolation unit for each 
output channel. Operating in Download and 
Streaming mode (continuous downstreaming of 
pulses from connected computer). MC_Stimulus II 
program with advanced features. 

Содержание MEA 1060-Inv

Страница 1: ...i MEA Amplifier for Inverse Microscopes Manual ...

Страница 2: ...he publisher and the author be liable for any loss of profit or any other commercial damage caused or alleged to have been caused directly or indirectly by this document 2020 Multi Channel Systems MCS GmbH All rights reserved Printed 06 11 2012 Multi Channel Systems MCS GmbH Aspenhaustraße 21 72770 Reutlingen Germany Phone 49 71 21 90 92 5 0 Fax 49 71 21 90 92 5 11 sales multichannelsystems com ww...

Страница 3: ...Noise Level 11 Operating the MEA Amplifier 13 Signal Amplification and Filters 13 Temperature Control 14 Mounting the MEA Probe and Grounding the Bath 15 Stimulation 17 Service and Maintenance 18 Troubleshooting 19 About Troubleshooting 19 Noise on Single Electrodes 19 Unsteady Baseline 20 Artifacts on All Channels 21 Liquid Spilled onto Amplifier 22 Technical Support 22 Appendix 23 Technical Spec...

Страница 4: ......

Страница 5: ...n about the installation and first steps The printed manual and Help are basically the same so it is up to you which one you will use The Help offers you the advantage of scrolling through the text in a non linear fashion picking up all information you need especially if you use the Index and the Search function If you are going to read larger text passages however you may prefer the printed manua...

Страница 6: ......

Страница 7: ...han on conclusion of the contract Multi Channel Systems MCS GmbH makes no guarantee as to the accuracy of any and all tests and data generated by the use of the device or the software It is up to the user to use good laboratory practice to establish the validity of his findings Guarantee and liability claims in the event of injury or material damage are excluded when they are the result of one of ...

Страница 8: ...cident prevention regulations and those of the employers liability associations are observed Each time before starting up make sure that the mains supply agrees with the specifications of the product Check the power cord for damage each time the site is changed Damaged power cords should be replaced immediately and may never be reused Check the leads for damage Damaged leads should be replaced imm...

Страница 9: ...cal pass bands would be 1 to 300 Hz for recording field potentials and 300 to 3000 Hz for recording action potentials It is also possible to use a broadband amplifier and filter the data with the digital filter of the free MC_Rack program Gain settings from 100 to 5000 are possible The amplifier is connected to the data acquisition computer via a single 68 pin MCS standard cable The analog output ...

Страница 10: ...rnal power supply for example a PS40W for operating the amplifier s Please ask your local retailer for more information on setup options Using the MC_Card for data acquisition Connect the MEA amplifier s output to the MC_Card input labeled MC_Card Ch 01 64 with a 68 pin MCS standard cable If you have a MEA120 System connect the second MEA amplifier with a 68 pin MCS standard cable to the input for...

Страница 11: ... 1 nF capacitor between bath and electrode Use MC_Rack or your custom data acquisition program to record from the test model probe and to check the amplifier Setting up MC_Rack Please refer to the MC_Rack manual for more information 1 Start MC_Rack 2 Open the file MEA_Display rck on the installation volume This basic rack contains the virtual MC_Card instrument with appropriate gain settings and a...

Страница 12: ...r Inverse Microscopes Manual 12 The following screen shot shows a recording from a typical MEA amplifier with a test model probe and a sampling rate of 25 kHz Double click a channel in the display to have a closer look ...

Страница 13: ...ns but MCS can also provide amplifiers with a gain of your choice from 100 to 5000 as well For large signals for example from whole heart preparations you need a lower gain to prevent a saturation of the amplifier Please note that the gain is a fixed hardware property and that you cannot change the gain of the amplifier by software controls For more information on the technical specifications of y...

Страница 14: ...t is integrated into the amplifier and the perfusion cannula and are both controlled by a Temperature Controller Temperature Controller and perfusion cannula with heating element and sensor are either included in the MEA System or can be ordered separately You have several options regarding the culture chamber such as a semipermeable seal that guarantees stable environmental conditions For using t...

Страница 15: ...ing NMI on the MEA chip should be on the right side Otherwise the MEA layout will not match with the pin layout of the channel map in MC_Rack This is very important for MEAs with internal reference electrode for example 3 Replace the lid and close it carefully Do not unpress the Change MEA button until you have grounded the bath and replaced the shielding that is until the setup has been completel...

Страница 16: ...e that you can keep the culture chamber closed and sterile for example with MEA MEM semipermeable membranes Important If you use a MEA with internal reference electrode you have to connect pin 15 to ground with the provided cable Setting electrode 15 to ground in the MEA_Select program is not sufficient for grounding the bath 1 Attach the provided silver wire or pellet to the amplifier s ground an...

Страница 17: ...mbodied MEA Cultures Advances in Network Electrophysiology Using Multi Electrode Arrays M Taketani and M Baudry Springer Wagenaar D A Madhavan R Pine J and Potter S M 2005 Controlling bursting in cortical cultures with closed loop multi electrode stimulation J Neurosci 25 3 680 8 Stimulation with MEA electrodes You can use any MEA electrode for stimulation Simply connect the output channel s of yo...

Страница 18: ... pins can be easily damaged Do not damage the shafts of the pins as they have to be replaced by the manufacturer Replacing the contact pins 1 Disconnect the amplifier 2 Open the housing and place the cover upside down on a soft and flat pad or cloth 3 Grip the defective contact pin firmly with a pair of tweezers and carefully lift it up Make sure that you pull only in a vertical direction not side...

Страница 19: ...in the Manual Help Multi Channel Systems has put all effort into making the product fully stable and reliable but like all high performance products it has to be handled with care 6 2 Noise on Single Electrodes The noise level on single electrodes is significantly higher than 8 μV Possible causes The electrode or the contact pin of the amplifier may be defective To test this do the following 1 Ope...

Страница 20: ...ing Possible causes Bath electrode is not connected to ground Connect the internal or external bath electrode to one of the ground inputs of the amplifier as described in Mounting the MEA Probe AgCl bath electrode needs is not well chlorided Rechloride the electrode or use a new one The MEA has an internal reference electrode and pin 15 is connected to ground appropriately but the orientation of t...

Страница 21: ...plifier Use a shorter cable if possible or try another power supply with a higher output power MCS high grade cables with a total length of up to 3 m and the isolated power supply IPS10W are recommended for the MEA System You have connected too many amplifiers to your power supply The isolated power supply IPS10W that is integrated in your data acquisition computer if you use a MEA System can supp...

Страница 22: ... Support Please read the Troubleshooting part of the Manual Help first Most problems are caused by minor handling errors Contact your local retailer immediately if the cause of trouble remains unclear Please understand that information on your hardware and software configuration is necessary to analyze and finally solve the problem you encounter Important If you need to send the amplifier back to ...

Страница 23: ...0 Maximum recommended stimulus input voltage 5 V Number of analog input channels 60 Input voltage 4 mV to 4 mV with respect to the standard gain of 1200 Input impedance 1011 parallel to 10 pF Input noise 800 nVRMS Noise density en 15 nV Wurzel aus Hz Number of analog output channels 60 Output voltage 5 V Output current 10 mA Output impedance 300 Bandwidth 1 Hz to 3000 Hz standard 1 Hz to 5000 Hz c...

Страница 24: ...ng temperature Ambient temperature min 10 C to 50 C Accuracy 0 1 C in the range of 20 C to 40 C Recovery time 0 5 min to 2 min Calibration constant T c R R0 R0 for c 259 7 C Thermal resistance 6 C Watt Typical time constant 50 s Standard hole size for MEA1060 INV BC HE Inv 8 8 mm Optional hole sizes HE Inv 12 HE Inv 23 12 mm 23 mm Pin layout 7 1 3 Test Model Probe The provided test model probe sim...

Страница 25: ...fier The writing NMI should be on the right side The illustration shows the standard pin layout of the socket on the amplifier The list below shows the assignment of the electrodes to the pins of the socket Pin 1 is the ground of the power supply pin 2 is the ground of the signal that is where the signal is referred to Both pins are already internally connected inside the amplifier Channels A1 to ...

Страница 26: ...2 4 48 34 51 5 46 35 53 6 45 36 54 7 38 37 61 8 37 38 62 9 28 39 71 10 36 40 63 11 27 41 72 12 17 42 82 13 26 43 73 14 16 44 83 15 35 45 64 16 25 46 74 17 15 47 84 18 14 48 85 19 24 49 75 20 34 50 65 21 13 51 86 22 23 52 76 23 12 53 87 24 22 54 77 25 33 55 66 26 21 56 78 27 32 57 67 28 31 58 68 29 44 59 55 30 43 60 56 31 41 61 58 32 42 62 57 ...

Страница 27: ...1060 Inv MEA amplifier for upright microscopes MEA1060 Up Probe interface and 60 channel pre and filter amplifier with custom gain and bandwidth MEA amplifier with blanking circuit for inverted microscopes MEA1060 Inv BC MEA amplifier with blanking circuit for upright microscopes MEA1060 Up BC Probe interface and 60 channel pre and filter amplifier with custom gain and bandwidth The blanking circu...

Страница 28: ...uit for inverted microscopes USB MEA240 Inv 4 BC System MEA Workstation for recording and analyzing data from MEA micro electrode array experiments featuring stimulus artifact suppression blanking and software selectable recording and stimulating electrodes Data acquisition from up to 240 MEA electrodes 4 analog channels 1 audio channel and 16 digital channels Integrated analog digital converter b...

Страница 29: ...ing rate of up to 50 kHz per channel Accessories Product Product Number Description Adapter for MEAs with 8 special electrodes for stimulation MEA STIM Adapter For use with MEAs 200 30 Ti stim or 200 30 ITO stim and MEA1060 Inv amplifier Holder with M3 threads MPM3 For fixing tools with M3 threads next to the MEA 1 Channel temperature controller TC01 2 Channel temperature controller TC02 PID based...

Страница 30: ...ring contact comb GSCC1060 Up For MEA1060 Up MEA1060 Up BC amplifier 3 Pole cable from STG to MEA1060 C3P With ground not necessary for MEA1060BC amplifiers Red single pole cable from STG to MEA1060 CR Black single pole cable from STG to MEA1060 CB MEA Systems Product Product Number Description MEA recording system for inverted microscopes 60 electrode channels MEA60 Inv System Complete with 5 MEA...

Страница 31: ...02 power supply and accessories MEA recording system for inverted microscopes 120 electrode channels MEA120 Inv 4 System Complete with 5 MEAs data acquisition computer with MC_Card 4 x MEA1060 Inv amplifier MEAS4 2 2 x TC02 power supply and accessories MEA recording system for upright microscopes 120 electrode channels MEA120 Up System Complete with 5 MEAs data acquisition computer with MC_Card ME...

Страница 32: ......

Страница 33: ...33 Index ...

Страница 34: ...idth 8 Frequency band 8 G Gain 8 H Heating 9 I IPS10W 5 M MC_Card 5 MEA layout 20 mounting 10 Model Test Probe 19 Mounting MEA Probe 10 N Noise troubleshooting 14 Noise Level testing 6 P Pin layout 20 S Signal amplification 8 Signal to noise 8 Stimulation 12 T Temperature connecting the Temperature Controller 5 Temperature control 9 Trace List 12 ...

Страница 35: ...35 ...

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