11
• Filter selection:
Filter
Function
ND2 (T=50%)
For brightness adjustment in
photomicrography
ND4 (T=25%)
ND16 (T=6.25%)
Blue filter
(colour balance filter)
For routine microscopy and
photomicrography
Green interference
(546nm)
For phase contrast and contrast
adjustment with black and white
film
HE
(didymium filter)
For colour photomicrography
of HE stained specimen with
tungsten type film
• A diffuser is built into the base of the microscope.
3.11. Power Cord
• Connect the socket of the of the power cord to the AC inlet
on the rear of the base of the microscope. Plug in the other
end of the cord to an AC outlet with ground conductor.
4. Microscopy
4.1. Coarse And Fine Focusing
• Focusing is carried out with the coarse and fine focus knobs
at the left and right of the microscope stand.
• The direction of vertical movement of the stage corresponds
to the turning direction of the focus knobs.
• One rotation of the fine focus knob moves the stage 0.2mm.
The graduation on the fine focus knob is 2 microns.
• Never attempt either of the following actions, since doing so will
damage the focusing mechanism:
- Rotate the left and right knob while holding the other.
- Turning the coarse and fine focus knobs further than their limit.
4.2. Coarse Focus Torque Adjustment
• To increase the torque, turn the torque adjustment ring
located behind the left-hand coarse focus knob in the direction
indicated by the arrow. To reduce the torque, turn the ring in
the direction opposite to that indicated by the arrow.
4.3. Coarse focus knob lock
• The coarse focus knob lock makes the stage can fixed at any
position at which the specimen is in focus i.e. by using the
handle to lock the coarse focus knob.
• With the specimen in focus, turn the handle to fix the knob.
• When the coarse focus knob lock is in position, the stage
cannot be raised from that position.
However, the fine focus knob can move the stage regardless of
the limit but will only lower the stage.
• Lower the stage by using the coarse focus knob.
4.4. Optical Path Changeover Slider
• The optical path change over slider of the trinocular eyepiece
tube can be used to select the amount of light distributed be-
tween the trinocular eyepiece tube and the vertical phototube.
• When the change over slider is pushed in until it reaches the
limit, 100% of the light enters the observation tube. When the
changer over slider is pulled out to the limit, the ratio of light
entering the observation tube and phototube will be 0:100.
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