Molecular Devices SpectraMax 190, Руководство оператора

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Chapter 1
1-10 SPECTRAmax
190 Microplate Spectrophotometer Operator’s Manual
set point. The sensors, also mounted inside the chamber, measure the air tem-
perature. The temperature feedback closed-loop control algorithms compare
the measured air temperature inside the chamber against the temperature set
point and use the difference to calculate the heating cycles. This technique
results in accurate, precise control of the chamber temperature with a temper-
ature variation of the air inside the chamber of less than 1.0°C. (The tempera-
ture uniformity within the microplate will depend upon the design, materials,
and/or con
Þ guration of that component.)
Computer Control
The SPECTRAmax 190 is equipped with an 8-pin DIN RS-232 serial port
through which a computer can communicate with and control the instrument.
SOFTmax PRO

Molecular Devices’ SOFTmax PRO software is a highly integrated program
that can be used to control and collect data from the SPECTRAmax 190.
SOFTmax PRO makes it possible to access the full capabilities of the
SPECTRAmax 190. SOFTmax PRO allows you to:
• Read microplates for endpoint, kinetic, or spectral scans
- Use up to six wavelengths for endpoint and kinetic readings
- Perform Absorbance or %Transmittance readings in the 190- to 850-nm
range
- Extend kinetic run times up to 99 hours
- Select your own read intervals for kinetic runs
- Specify the duration for Automix before and between readings
- Read the whole plate or a subset of microplate strips
• Use PathCheck to normalize the absorbance readings in each microplate
well to a 1-cm pathlength
• Design microplate templates to simplify data reduction
- Identify groups of wells with labels of your choice
- Identify individual wells with unique names
- Blank the entire plate, groups, and/or individual wells
• Save instrument settings, template formats, and data analysis parameters
for microplates as assay protocol Þ les and recall them for later use
- Rapid instrument and analysis setup for repeated microplate assays
- Uniform analysis for equivalent microplates
• Turn the incubator on or off to control the temperature in the microplate
drawer