Molecular Devices SpectraMax 190, Руководство оператора

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Instrument Description
SPECTRAmax
190
Microplate Spectrophotometer Operator’s Manual 1-3
Introduction
General Overview
The SPECTRAmax™ 190 microplate spectrophotometer provides rapid
and sensitive measurements of a variety of analytes across a wide range of
concentrations. It measures the optical density (OD) of samples in 96-well
microplates at a selected wavelength for a single point in time (endpoint),
over a speci
Þ ed period of time (kinetic), or over a selected wavelength range
(spectral scan).
Figure 1.1: The SPECTRAmax 190 System
The on-board microprocessor calculates and reports the absorbance (or
% Transmittance) for each well of a microplate. Data from multiple wave-
lengths can be acquired during a single reading, if desired, and different
calculations can be made based on this data using SOFTmax PRO software,
including the subtraction of blanks, use of standard curves, etc.
Typical applications include DNA quantitation, endpoint assays (quantitation
of cytoproliferation by MTT reduction, colorimetric protein assays, and
endpoint ELISAs) and kinetic measurements (enzyme studies, such as
determination of the activity of enzymes released from cells, and kinetic
ELISAs).
All standard 96-well microplates, strip wells, and Þ lter-bottom microplates
can be used in the SPECTRAmax 190.
With SOFTmax PRO, the contents of the wells in a microplate can be mixed
automatically by shaking before each read cycle, making it possible to per-
form kinetic analysis of solid-phase, enzyme-mediated reactions (mixing is
not critical for liquid-phase reactions). The temperature of the microplate
chamber can also be regulated, if desired, from 4°C above ambient to 45°C.