Pellicon
®
3 Cassettes Installation and User Guide
9
water into the feed port of the
Pellicon
®
holder. The recommended
feed flow rate is 4 to 6 L/min/m².
6. Once the pump has slowly ramped
up to the set point, partially close
the retentate valve (V4) to achieve
the retentate pressure listed in the
table above.
7. Recirculate the cleaning solution for the
prescribed time period as noted in the
Cleaning Conditions Chart.
Note: If the system is complex and
has other associated manifolds,
ensure that all wetted surfaces in the
manifolds are exposed to the cleaning
solution. All valves that have been
exposed to process fluids should also
be exposed to the cleaning solution. It
is good practice to cycle (partially open
and the partially close) valves at least
twice over the course of the cleaning
cycle to ensure that all wetted internal
surfaces of the valve body are exposed
to the cleaning solution.
8. At the end of the cleaning
recirculation, direct the retentate
and permeate to drain and continue
to run until the tank is empty
(without running the pump dry),
then turn off the feed pump, fully
open the retentate and filtrate lines
and drain the retentate, filtrate
piping and permeate piping.
9. Repeat the flushing procedure after
cleaning to remove chemical residue
from the cassette and system
piping.
Primary Cleaning Agents and Conditions for Organics, Biofilms,
Biopolymer, Proteins and Polyphenolic
Membrane
Cleaning
Agent
Concentration Temperature
(°C)
pH
Time
(min)
Ultracel
®
NaOH
0.1 N - 0.5 N
25 - 50
13 - 13.7
30 - 60
Biomax
®
NaOH
0.1 N - 0.5 N
25 - 50
13 - 13.7
30 - 60
NaOCl
200 - 500
ppm
25 - 50
10 - 11
30 - 60
Stable NWP from run to run is achievable after initial exposure. There is an initial
NWP decline in Pellicon
®
3 Cassettes after initial exposure to 0.5N NaOH. Better
membrane life has been observed at lower concentrations. Total exposure time of
Pellicon
®
3 Cassettes with Ultracel
®
membrane must not exceed 100 hours. Total
exposure time of Pellicon
®
3 Cassettes with Biomax
®
membrane must not exceed 200
hours. Contact Technical Service for additional Cleaning Agents and Conditions.
Sanitization
Sanitization should be performed after the Pellicon
®
3 Cassette installation has been
thoroughly cleaned and flushed to reduce bioburden. Sanitization pressures, flow rates
and volumes are identical to those used during Cleaning. Repeat the flushing procedure
after sanitization to remove chemical residue from the cassette and system piping.
NOTE
Use sanitization agents and conditions that are suitable for the process
requirements. Sanitization agents must comply with applicable local regulations.
Recommended Sanitization Conditions and Agents
Membrane
Sanitizing Agent
Concentration
Temp
( ° C)
pH
Time
(min)
Biomax
®
NaOCl
200 - 500 ppm
25 - 50
10 - 11
30
Peracetic acid-containing
Cold Sanitant*
1%
10 to 40
3.5
30
NaOH
0.1 N - 0.5 N
25 - 50
13 - 13.7
30
Ultracel
®
Peracetic acid-containing
Cold Sanitant*
1%
10 - 40
3.5
30
NaOH**
0.1N
25 - 50
13
30
