Pellicon
®
3 Cassettes Installation and User Guide
13
NOTE
If the system is complex and has other associated manifolds, ensure that all
wetted surfaces in the manifolds are exposed to the solution. All valves that have
been exposed to process fluids should also be exposed. It is good practice to
cycle (partially open and the partially close) valves at least twice over the course
of the cycle to ensure that all wetted internal surfaces of the valve body are
exposed to the solution.
8. Filters may be stored in holders with the feed, retentate and filtrate isolation valves
closed and enough compression on the cassettes to ensure that the fluid cannot
escape or evaporate.
9. The filters may also be removed from the system and placed in sealed bags
or containers. Submerge in storage solution in appropriate container. Filters
should be kept at 2-8 ºC. Process holders with hydraulics can properly hold and
maintain storage solution. Process holders are also designed with a store mode
compression setting that releases to about 60% of the process compression and
ensures that the units will not leak externally.
NOTE
The storage agent used must comply with applicable local regulations.
Recommended Storage Solutions for Pellicon
®
3 Cassette Filters
Membrane
Storage Agent
Concentration
pH
Recommended
Time Period
Ultracel
®
NaOH*
0.05 N
12.7
1 year
NaOH
0.1 N
13
6 months
Biomax
®
Acetic/Phosphoric Acid 0.16M Acetic/0.12M
Phosphoric Acid
2 - 3
1 year
NaOH
0.1 N
13
1 year
*Use milder conditions if effectiveness of agent is still observed. Better membrane life
is expected with lower concentration of caustic and/or at 20-25 °C for regenerated
cellulose membranes with a nominal molecular weight limit of 3 or 5 kDa.
These recommended storage agents will ensure membranes remain wet and will
prevent microbiological growth without damage to the filter. Upon reinstallation, the
filters should be flushed, cleaned and sanitized prior to use.
Filters should be stored in liquid-tight bags or in containers at 2 - 8 °C and should
not be frozen. Fresh solutions should be prepared after the recommended time period
to prevent possible microbial growth or
contamination.
Ultracel
®
membranes can be stored in
0.1 N NaOH only for the recommended
time period. Longer exposure may result in
membrane damage.
