4.1 Operation
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
22
￭￭￭￭￭￭￭￭
IC equipment Dialysis 6.5330.100
2 Preparing the sample
￭
To avoid blockage of the sample channel, always centrifuge sam-
ples with a high content of suspended or solid particles in a tab-
letop centrifuge for 5 minutes at 10,000 rpm.
3 Putting the IC system into operation
￭
Switch on all required devices.
￭
Start the software and load the required IC system.
￭
Condition the IC system.
4 Putting the dialysis system into operation
￭
Immerse the aspiration capillary for the acceptor solution in the
acceptor solution.
￭
Immerse the sample aspiration capillary in a rinsing solution (e.g.
ultrapure water).
￭
Start both peristaltic pumps in the software.
￭
Rinse the dialysis system with the acceptor solution and the rins-
ing solution for approx. 10 minutes and then switch off the peri-
staltic pumps again.
5 Calibrating the IC system
￭
Immerse the sample aspiration capillary in the standard solution.
￭
Start the determination in the software and wait until the stan-
dard chromatogram has been evaluated.
￭
Repeat the two steps for all standard solutions.
6 Determining the sample
￭
Immerse the sample aspiration capillary in the sample solution.
￭
Start the determination in the software and wait until the chroma-
togram has been evaluated.
7 Rinsing the dialysis system
￭
After the end of the measurements, immerse the sample aspira-
tion capillary in the rinsing solution.
￭
Switch on the peristaltic pump for the sample channel in the soft-
ware.
￭
Rinse the dialysis system with the acceptor solution and the rins-
ing solution for approx. 10 minutes and then switch off the peri-
staltic pumps again.
