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Cellicon

Perfusion Solution for Process Development User Guide

Ending the Run

Disposing of the Filter

The filter is completely single use and should be disposed of after use.

Follow company procedure for waste disposal.

Cleaning the Hardware

All parts of the system should be wiped down with a cloth dampened

with a 70% ethanol or isopropyl alcohol solution.

Содержание Milipore Cellicon Perfusion Solution for Process Development

Страница 1: ...n Solution for Process Development User Guide Contents Introduction 2 System Components 2 Setting Up the System 3 Starting the System 4 User Interface 4 Settings 7 Running the Pump 7 Alerts 9 Settings...

Страница 2: ...scribes the installation setup and use of the CelliconTM Perfusion Solution for Process Development This system is for industrial use only and cannot be used in a residential environment System Compon...

Страница 3: ...ors on the assembly as follows Press 1 to the feed line Press 2 to the retentate line Press 3 to the perfusate permeate line Connecting the Bioreactor Use a tubing welder to connect The cell retention...

Страница 4: ...on Powering up the system launches the perfusion control software The initial screen is the username selection screen After selecting the username the password screen will appear Touch the white box o...

Страница 5: ...ton Selecting the Settings button opens the settings screen User Icon and Time Date Display Selecting the user icon allows the user to lock the screen or switch users Lock screen will cause the contro...

Страница 6: ...low Setpoint Prime System Zero Adjust Flow Rate Speed Setpoint Pump Start Decrease Flow Rate Increase Flow Rate Status The current values state of the system components and data logging switch are dis...

Страница 7: ...ton will read START PUMP If the pump is on the button will read STOP PUMP Stopping the pump will cause a confimation screen to appear Prime System The pump needs to be primed to reduce damage to cells...

Страница 8: ...t be stopped A pop up will appear to confirm that the pump is stopped Flowmeter 2 does not require that the pump is stopped All sensors should be zero adjusted prior to starting a run Only one sensor...

Страница 9: ...splayed Alarm Acknowledgement Alerts can be removed from the main screen by selecting Acknowledge from the alert bar or in the alert log The alert will reappear on the alert bar if it remains active 1...

Страница 10: ...ill make a loud beep when the alert is activated Select Dismiss to return to the main screen then select Acknowledge on the alert bar or within the alert log The data region can still be viewed when a...

Страница 11: ...Critical Alarm High Optional 1 Pressure Alarms Pressure Critical Alarm Low Optional 1 Pressure Warning Alarm Low Never 2 Pressure Warning Alarm High Never 2 Pressure Critical Alarm High Optional 1 Al...

Страница 12: ...ending is fixed and generated with the date followed by the time when the logfile is created When data logging is switched On a prompt to enter a filename will appear This filename will replace the t...

Страница 13: ...error The resolution of the flow signal can be improved by decreasing the full scale of the flowmeter The resolution of the flow signal is always 1 10 000 of the full scale Decreasing the full scale b...

Страница 14: ...Refer to Table of Alerts for more information Critical alarms are shown in red color type 1 alert Warning alarms are shown in yellow color type 2 alert Neither critical nor warning alarms will shut d...

Страница 15: ...nalog Input PLC Digital Input PLC Analog Output User Accounts Settings The administrator can generate delete and edit user accounts including password protection capabilities and rescrict access permi...

Страница 16: ...s have been added to the reactor This will eliminate the possible introduction of cell shear when the system is primed see Priming Preparation Equipment Needs The following user supplied items may be...

Страница 17: ...r or gas in the pump head will negatively affect pump performance and decrease the recirculation flow rate MobiusTM 3L Single use Bioreactor When using the MobiusTM single use 3L reactor the inlet to...

Страница 18: ...lect Prime system on the main menu screen This will switch the controller into Speed setpoint mode and provide step by step guidance on how to complete the priming 2 Ensure that the perfusate permeate...

Страница 19: ...ved on the USB drive the log interval and the log file size Choose a log interval of 60 seconds or greater and a log file size of 65000 lines Insert the USB drive into a computer to view data Open Mic...

Страница 20: ...ars light grey and does not read values Connect cables from component to controller Install correct software Component is in an alarm state appears red or yellow Plug in component cables Pump alert Pl...

Страница 21: ...d ensure it does not pull air from sparger Check system mode flow control vs speed control No flow through permeate Ensure permeate line is not clamped or pinched Symptom Corrective Action Early filte...

Страница 22: ...un Disposing of the Filter The filter is completely single use and should be disposed of after use Follow company procedure for waste disposal Cleaning the Hardware All parts of the system should be w...

Страница 23: ...s All other trademarks are the property of their respective owners Detailed information on trademarks is available via publicly accessible resources MK_UG5285EN Rev 1 06 2020 2020 Merck KGaA Darmstadt...