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LuminUltra GeneCount

®

 Q-8 Q-16 

qPCR Non-Touch Screen Version

 

 

For Windows® - based Software 

 

Version 0.16 – for Beta release 

 

 

 

 

 

 

 

 

 

 

 
 

 

 

 

 

 

 

 

 

 

 

 

 

  

Содержание GeneCount Q-16

Страница 1: ...LuminUltra GeneCount Q 8 Q 16 qPCR Non Touch Screen Version For Windows based Software Version 0 16 for Beta release...

Страница 2: ...10 Install the software 10 Launching the software 12 The home button 12 Resizing the window 12 The setup page name setup 13 Samples and fluorescence channels set up 13 Open existing experiment for tem...

Страница 3: ...based on user feedback Newest versions and information are available for download from the website my luminultra com and from sales LuminUltra com Note Very important information is contained within...

Страница 4: ...rument If the power cable is damaged it should be replaced with a new one of the same specifications The power supply wire should be treated with care do not place objects on the wire and do not place...

Страница 5: ...tes a strange odour The instrument is dropped or its casing is damaged There is an obvious change in the function of the instrument Warning When operating or performing work on the device all relevant...

Страница 6: ...re is no guarantee that interference will not occur in a particular installation If this equipment does cause harmful interference to radio or television reception which can be determined by turning t...

Страница 7: ...ick Guide For Windows based Software 7 Overview This is a user s guide for using LuminUltra GeneCount qPCR with Windows based software Parts required GeneCount qPCR instrument unit AC adapter and powe...

Страница 8: ...USB 2 0 Wi Fi Excitation source High endurance LED Detector Ultra low light CMOS Bio imaging chip Thermal system Solid state Peltier based Tube plate formats 0 2mL 4 or 8 tube strip Reaction volume 1...

Страница 9: ...ix at end of the document about how to download decompress and install software from LuminUltra s web site Connect instrument with Windows based PC via included USB cable Power up the instrument using...

Страница 10: ...pport print report function 2 We use the USB HID device interface for GeneCount This is the same USB HID for keyboard and mouse Step by step software guide Install the software The Beta version of the...

Страница 11: ...LuminUltra GeneCount Q 8 Q 16 qPCR Quick Guide For Windows based Software 11...

Страница 12: ...eral notable features are marked in Figure 1 Marker 1 is an indicator of whether the GeneCount USB device is found If found a message HID Device Connected is shown along with a green dot We call it HI...

Страница 13: ...ed instrument can support Each reaction well can be associated with a sample In this page the user can enter a name for each sample It a sample well name field is left empty white spaces it is conside...

Страница 14: ...d are configurable A cycler program can have several periods and each period can have one or more steps For example we could have pre denature period cycle period and hold period In the cycle period w...

Страница 15: ...nal to noise ratio signal quality We recommend leaving this check box checked Running the experiment and monitor status In the setup page cycler setup when we are satisfied with all the settings and l...

Страница 16: ...s Ct Threshold The threshold for determining Ct is expressed as the percentage of the saturating level of the amplification levels after normalization The default Ct threshold is 10 The bigger this pe...

Страница 17: ...LuminUltra GeneCount Q 8 Q 16 qPCR Quick Guide For Windows based Software 17...

Страница 18: ...dditional parameters that justifies the positive negative test These parameters include the saturation fluorescence level amplification efficiency and fluorescence reading signal to noise ratio False...

Страница 19: ...ve to first the select the specific channel and perform such analysis Figure 7 Standard curve analysis page Steps to perform standard curve analysis Step 1 Select the fluorescence channel for such ana...

Страница 20: ...riment file will contain all the settings and experiment results Two report formats will be used for report generation The excel file report is hand editable The PDF report is easier to store and use...

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