LuminUltra GeneCount
®
Q-8 Q-16 qPCR
Quick Guide For Windows® - based Software
18
Positive vs negative results
For each sample’s DNA target, we get a positive vs negative result. Generally, the negative result
will be shown as “Neg” and the positive result will be shown with a Ct value between Ct low limit
and maximum cycle number. If we mouse over the Ct value in the data table, we will see some
additional parameters that justifies the positive/negative test. These parameters include the
saturation fluorescence level, amplification efficiency and fluorescence reading signal to noise
ratio.
False positive results
Sometimes a negative result is the result of a “false positive” result. If we mouse over the result, we
see that the “Confidence” level of positive is below a threshold, for example, 10%. The confidence
threshold is adjusted through advanced setting window.
Further discussion of how to judge positive vs. negative results is given in Appendix B.
Figure 6. Positive vs Negative analysis
