Logos LUNA-FL, Руководство пользователя

Страница: 103 / 122

103
www.logosbio.com
8.3 Adjusting Parameters for Yeast Cell Counting
<Yeast Counting Parameters on the Protocol Menu>
The adjustable parameters are described as follows:
Parameter Range Default Description
Dilution
Factor
1-10 1.11
The default value for the dilution factor is “1.11”.
To perform the yeast counting, 1 µl of FDA and 1 µl of PI
is added to 18 µl of the yeast sample. The dilution factor
in this case is calculated as 1.11 (20/18). In case 2 µl of
each FDA and PI is added to 16 µl of the cell sample, the
dilution factor is 1.25 (20/16). Likewise, the dilution factor
changes from 1.11(2 µl of the FDA/PI cell viability kit
used), 1.25 (4 µl), 1.42 (6 µl), 1.66 (8 µl), 2.00 (10 µl),
2.50 (12 µl), 3.33 (14 µl), 5.00 (16 µl) to 10.00 (18 µl).
Dilution factor corrects the actual number of cells; thus
the analyzed results are generated for the original
samples.
Minimum
Cell Size
1-90 1
For yeast, the default min value is 1. The unit is 1
micrometer.
Maximum
Cell Size
1-90 20
For yeast, the default max value is 20. The unit is 1
micrometer.
Green
Fluorescence
Threshold
1-10 2
Green and Red Fluorescence Threshold will determine
the level of threshold during the image processing.
Increased threshold will detect less cells by subtracting