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Chapter 5
– GFP Transfection Assay
5.1 Sample Preparation
5.1.1 Perform a GFP transfection experiment with the appropriate negative controls, e.g., non-
transfected control or mock-transfected control without plasmid
5.1.2 Harvest the cell samples approximately 24 - 96 hours after the transfection.
5.1.3 Count the number of cells in the sample. The optimal cell concentration for cytometry is generally
between 2 x 10
6
– 5 x 10
6
cells/mL. If the initial concentration is too high, add some cell culture medium
to dilute. If the initial concentration is too low, centrifuge the cell suspension and resuspend the cells in
a smaller volume to raise the cell concentration.
5.1.4 Turn on the LUNA-FL
™ instrument and prepare the following items in order to measure GFP
transfection efficiency.

Cell sample
 PhotonSlide™ (Cat No. L12005) or LUNA™ Reusable Slide (Cat # L12008)

A USB drive to save data and results, if desired.
Note : The reagents and kits for the GFP transfection assay are not included in the LUNA-FL
™ package.
They must be purchased separately from other suppliers.