23
Leica RM2125
5.6 Sectioning
Always rotate the handwheel evenly with the rotation speed of the handwheel depending on
the hardness of the specimen being sectioned.
Harder specimens should be selected at slower speed.
5.7 Changing specimen
Lock the handwheel and cover the knife edge with the knife guard prior to any manipulation of
knife or specimen, prior to changing the specimen block and during all work breaks!
•
Bring specimen to uppermost position (via handwheel) and lock the
handwheel in 12 o’clock position.
•
Cover the blade edge with the knife guard.
•
Remove the specimen from the specimen clamp and insert a new
specimen block.
•
Use the coarse feed wheel to move the specimen clamp backwards far
enough to be able to start trimming the new specimen block.
•
For that purpose - if you work with a knife holder with lateral adjust-
ment option - move the knife holder base / knife holder to the desired
position (see
chap. 5.8.7
) or - if your knife holder base is without lateral
adjustment - move the knife or blade itself.
•
Start sectioning by rotating the handwheel (
15
) evenly in clockwise di-
rection.
•
Pick up the sections and mount them on microscope slides.
5.
Operation
•
Use the adjusting knob (
33
) on the right side
of the microtome front panel to selected the
desired section thickness unless the appro-
priate value already shows in window (
34
).
The line of the graduated scale that coin-
cides with the red reference mark (
38
) corre-
sponds to the thickness value selected.
•
For sectioning use another part of the blade
edge than the one used for trimming.
Fig. 17
34
33
38
Содержание RM2125
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