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Leica M Series User Manual

Quick Start Guide 59

The iris diaphragm in the optics carrier of your
M series microscope has the same purpose as
those in a camera shutter: it regulates the avail-
able light, which changes the depth of field. The
"depth of field" is the area of a specimen that is
brought into sharp focus.

Closing the iris diaphragm

O

Close the iris diaphragm by turning the
knob to the left. The subject appears darker
and the depth of field increases.

Opening the iris diaphragm

O

Open the iris diaphragm by turning the
knob to the right. The subject now appears
brighter, but the depth of field decreases.

Iris Diaphragm

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Содержание M125 C

Страница 1: ...Leica M125 C Leica M165 C Leica M205 C Leica M205 A User Manual...

Страница 2: ...emicals to clean colored surfaces or accessories with rubberized parts This could damage the surfaces and specimens could be contaminated by abraded particles O O In most cases we can provide special...

Страница 3: ...onal safety maintenance and accessories The Safety concept booklet contains addi tional safety information regarding the service work requirements and the handling of stereo microscope accessories and...

Страница 4: ...indicates especially impor tant information that if not observed can cause the following O O Hazards to personnel O O Functional disturbances or damaged instruments Danger due to hot surface This symb...

Страница 5: ...re outside of the scope of this manual Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims Place of use O O Refer to Safety Concept booklet O O...

Страница 6: ...s O O Refer to Safety Concept booklet Disposal O O Refer to Safety Concept booklet Legal requirements O O Refer to Safety Concept booklet EC Declaration of Conformity O O Refer to Safety Concept bookl...

Страница 7: ...her defective nor interrupted since otherwise a malfunction poses a risk of fatal injury Never unplug the power plug with moist or wet hands There is danger of an electric shock Instrument safety and...

Страница 8: ...ug NEMA 5 15P or unscrew any mechanical components unless expressly instructed to do so in the instructions The microscope must be connected to a grounded outlet Always position the microscope so that...

Страница 9: ...f the Focus Drive 55 Changing the Magnification Zoom 56 Ratchet Steps and Magnification Levels 57 Parfocality More Comfort and Convenience for Your Work 58 Iris Diaphragm 59 General Instructions 2 Saf...

Страница 10: ...ansmitted light Base Leica TL BFDF Controls 79 Transmitted light Base Leica TL BFDF Operation 80 Leica IsoPro Non motorized Controls 81 System Illumination Leica LED5000 MCI 83 Leica LED5000 RL 85 Acc...

Страница 11: ...design ensures that the M series adapts perfectly to your needs no matter which accessories you require for your tasks Though the reliability and robustness of Leica stereo microscopes is legendary li...

Страница 12: ...en by the observer becomes distorted a sphere becomes an ellipse a flat surface curves toward the observer Limits are made to be broken The Leica M205 C is the world s first stereo microscope with a z...

Страница 13: ...parameters such as the magnifica tion the illumination the position of the iris diaphragm and more to the Leica LAS X soft ware Thus you always know the conditions under which an image was acquired Fe...

Страница 14: ...ything is Relative The Leica M series provides maximum flexibil ity in choosing equipment thanks primarily to the modular configuration and the compatibil ity that Leica has painstakingly maintained f...

Страница 15: ...focus If you prefer you can continue to use the previ ous objective series In this case parfocality is no longer guaranteed Tubes The interface between the optics carrier and the tube has remained th...

Страница 16: ...assembled and commissioned by your Leica consultant click here to skip through the instal lation instructions and go directly to the Quick Start Guide on page 49 If on the other hand you are assemblin...

Страница 17: ...Leica M Series User Manual Assembly 17 Assembly...

Страница 18: ...e corresponding base Tools used O O Hex socket screwdriver 3mm Assembling the column adapter 1 Securely install the column adapter on the column using the four included screws Assembling the focusing...

Страница 19: ...ding base Tools used O O Hex socket screwdriver 3 mm Assembling the column adapter 1 Securely install the column adapter on the column using the four included screws Assembling the focusing column 2 S...

Страница 20: ...ht base the focus ing column and motorized focus are installed directly on the base no extension plate is required Tools used O O Hex socket screwdriver 3 mm Assembly 1 Place the focusing column on th...

Страница 21: ...0 to switch off the device Assembling the optics carrier 1 Place the optics carrier on the focusing column so that the screw fit into the thread provided and the lug fits into the groove 2 Press the o...

Страница 22: ...ons 1 Unscrew the positioning screw and remove the protective cover Assembling the tube 2 Push the tube for example the inclined binocular tube into the dovetail ring and rotate it slightly in both di...

Страница 23: ...lable 10 16 25 and 40 wide field eyepieces for persons wearing glasses Preparation 1 If you want to use an optional graticule insert it now page 65 2 Remove the plastic tube guard Inserting the eyepie...

Страница 24: ...sem bly and disassembly so that it does not fall onto the stage plate This applies particu larly to the 2 planapochromatic objective which is very heavy Remove all specimens from the stage plate first...

Страница 25: ...0 and attach the objective nosepiece to the drive hous ing from the front Screw the objective nosepiece firmly into place 3 Unscrew the three Phillips screws on the objective mount of the optics carri...

Страница 26: ...aration O O Open the iris diaphragm O O Set the dioptric correction of the eyepieces to 0 Adjustment 1 Rotate the 2 objective into the beam path and set it to the lowest magnification 2 Focus on the s...

Страница 27: ...Check the switch position O O If the switch position corresponds to the optics carrier used skip the following steps Otherwise proceed as follows 2 Unscrew the two screws that hold the switch in posi...

Страница 28: ...ponding optics carrier it can be installed 1 Move the slide of the AX carrier into mid position 2 Install the AX carrier on the focusing column 3 Screw the objective to the AX carrier 4 Unscrew the th...

Страница 29: ...ransport anchors Before you can use the transmitted light base for the first time it is abso lutely necessary to remove the two transport anchors as follows Transmitted light Base Leica TL BFDF Before...

Страница 30: ...0 562 The selected stage is mounted on the base before commissioning You can switch between the stages at any time with just a few hand movements The following paragraph assumes use of the base withou...

Страница 31: ...t or the right side of the XY stage If the controls are to be mounted on the left hand side the gear rod on the bottom side of the XY stage must be unscrewed and reattached in reverse 1 Take the glass...

Страница 32: ...the glass plate from the XY stage and turn it around 2 Attach the axis with the control buttons to the desired side The fastener snaps into the XY stage magnetically 4 Attach the axis with the two All...

Страница 33: ...stage all the way back towards the column 5 Reinsert the glass plate back into the stan dard stage Focusing drive and column 1 Unscrew the extension plate from the base using the Allen key provided 2...

Страница 34: ...ace to the optics carrier is on the column The terminals The connection to the PC and to other instru ments is made using the terminals on the rear side of the column 1 3 CTL2 terminals for auxiliary...

Страница 35: ...amera can be stowed in the cable duct Feeding the cables 1 Unscrew the three screws on the cable duct 2 Remove the cover of the cable duct 3 Place the cables in the cable duct and screw the cover on t...

Страница 36: ...sembly 36 Cables Diagram SmartTouch PC USB Power USB LED5000 RL LED5000 MCI Please use the Leica Application Suite X to configure the functions of the system Alternative installation 115 230 V LED5000...

Страница 37: ...Constraints TheLeicaLED5000MCIcannotbeusedtogether with the objective nosepiece Assembly 1 Hold the LED5000 MCI with one hand and tightly screw the retraining stirrups on both top holes on the drive...

Страница 38: ...emain the same For such purposes the Leica LED5000 MCI is directly fastened to the column Installation on the column 1 Pull the retaining stirrup out of the Leica LED5000 MCI 2 Screw the retaining sti...

Страница 39: ...planapochromat 1 and planapochromat 0 63 objectives With all other objectives the working distance is too low for adequate illumination The ring illuminator cannot be used together with the objective...

Страница 40: ...ys for example if simultane ously using the Leica LED5000 MCI system illu minator In this case the ring illuminator cannot be connected directly to the Leica LED5000 MCI If you work with the manual fo...

Страница 41: ...larization set into place firmly using the metal plate provided O O The optional polarization set polarizer and analyzer is attached and screwed in below the ring illuminator O O The optional diffuser...

Страница 42: ...ont keypad O O Use the key to switch the illuminator on or off O O Use the or keys to adjust the bright ness in 10 increments O O Tap the or key briefly to adjust the intensity in small increments Hol...

Страница 43: ...ress one of these keys again If you change the segment using the key the automatic changeover is kept Pressing or briefly stops the changeover O O Press and hold the key for approx 2 seconds to switch...

Страница 44: ...he Leica LED5000 NVI in factory configu ration is already assembled with the exten sion plate for assembly on manual and motorized focusing columns at the factory 2 Place the Leica LED5000 NVI on the...

Страница 45: ...e optics carrier on the adapter of the Leica LED5000 NVI and tighten it with an Allen key M4 6 Install the remaining microscope compo nents tube ergonomic accessories camera etc according to the corre...

Страница 46: ...10 411 597 Achromatic objective f 100 mm 10 441 787 Achromatic objective f 150 mm 10 431 692 Achromatic objective f 175 mm 10 382 162 Achromatic objective f 200 mm 10 382 172 Achromatic objective f 4...

Страница 47: ...e equipped with an optional polarization set Using the polariza tion set reflections can be reduced on metallic surfaces for example 1 The polarizer is inserted into the filter insert on the bottom of...

Страница 48: ...1 2 can be used Make sure that the filters being used are suffi ciently temperature resistant so they are not damaged by the high energy illumination Additional empty filter inserts 10 725 035 can be...

Страница 49: ...Leica M Series User Manual Quick Start Guide 49 Quick Start Guide...

Страница 50: ...get right to work Therefore your next step should be to study the Quick Start Guide which outlines the most important steps at a glance This manual will then familiarize you with the finer details of...

Страница 51: ...stops on off 3 Coarse fine focusing 4 Fastening screw for the binocular tube or accessories 5 Interchangeable objective 6 Adjustable eyepiece tubes 7 Eyepieces for spectacle wearers with dioptric cor...

Страница 52: ...this Not to worry after a little while it will become automatic Reference value The distance between eye and eyepiece measures approx 22mm for 10 23B wide field eyepieces for persons wearing glasses A...

Страница 53: ...eyepiece Using the Eyepieces If you do not wear glasses 1 Hold the eyepiece firmly and rotate the eyecups forwards counterclockwise 2 If an eyepiece is equipped with the inte grated dioptric correctio...

Страница 54: ...of the objective O O The focusing drive can be operated either left or right handed Focusing O O The inner coarse adjustment is used for covering great distances O O The outer fine adjustment is used...

Страница 55: ...ocus movement too loose or too tight Does the outfit tend to slide downwards The resistance can be adjusted individually depend ing on the equipment weight and personal preferences as follows 1 Grip t...

Страница 56: ...me indicates the zoom range covered O O Leica M125 C 12 5 1 O O Leica M165 C 16 5 1 O O Leica M205 C 20 5 1 Leica M205 A 20 5 1 The rotary knob for the zoom can be used either left or right handed Zoo...

Страница 57: ...results etc can be reproduced more accurately Enabling and disabling ratchet steps 1 Push the top button downwards to disable the ratchet steps 2 Push the bottom button upwards to enable the ratchet s...

Страница 58: ...t is located higher or lower Requirements for parfocal work O O If you are using an eyepiece with dioptric correction the procedure differs from this description For more information refer to page 64...

Страница 59: ...the depth of field The depth of field is the area of a specimen that is brought into sharp focus Closing the iris diaphragm O O Close the iris diaphragm by turning the knob to the left The subject ap...

Страница 60: ...Leica M Series User Manual Eyepieces 60 Eyepieces...

Страница 61: ...ro scope but also has a critical effect on the maximum magnification The magnification factor is between 10 and 40 The following eyepieces are available for the M series Magnification Dioptric correct...

Страница 62: ...epieces is a potential transmission method for bacterial and viral infections of the eye The risk can be kept to a minimum by using individual eyepieces or detachable eyecups Eyecups can be ordered se...

Страница 63: ...earing your glasses look through the eyepieces and focus on the specimen 3 Rotate both eyepieces to the maximum value of 5 4 Hold one eye closed and rotate the other eyepiece in direction until the sp...

Страница 64: ...orrect setting of the diopters and the parfocality The following adjustments only have to be carried out once by each user Preparations O O Move the lever of the video phototube to the observation pos...

Страница 65: ...ten ing it using the clamping screw Use with the AX carrier If possible measure with the micro scope carrier AX in vertical position The measurements are more accurate without the convergence angle in...

Страница 66: ...Leica M Series User Manual Photography Video 66 Photography Video...

Страница 67: ...stems offers a variety of adapters that can be used together with the 50 and 100 trinocular tubes Leica cameras However if you require absolute control over the camera and need the capability for meas...

Страница 68: ...of the field of vision Cameras from third party suppliers In addition to Leica cameras with the standardized C mount interfaces you can connect third party cameras to the microscope using a T2 bayone...

Страница 69: ...tube 50 enables you to simultaneously view and photograph a specimen The available light is divided as follows O O 50 is available for the two eyepieces O O 50 of the light is diverted to the video ph...

Страница 70: ...sten the trinocular tube 100 to the optics carrier instead of the binocular observation tube refer to page 22 Switchover O O Turn the controller on the right side of the tube into the horizontal posit...

Страница 71: ...es to best fit their posture The photo slider switches the right beam path from 100 of light to the eyepieces to 100 light to the camera port In photo position the left beam path is closed to prevent...

Страница 72: ...Leica M Series User Manual Microscope Carrier 72 Microscope Carrier...

Страница 73: ...l view and tighten the clamping screw The Microscope Carrier AX Users who intend to do a great deal of photog raphy take measurements or work with polar ization should select the microscope carrier AX...

Страница 74: ...ill go and tighten the clamping screw The objective is now located directly beneath either the left or the right beam path Coaxial light and the Leica M205 C Leica M205 A When using coaxial incident l...

Страница 75: ...es objectives the parfo cality is maintained even when objectives are changed meaning that the specimen remains in sharp focus during the change Older Leica objectives can continue to be used but with...

Страница 76: ...Leica M Series User Manual Objectives and Optical Accessories 76 Objectives and Optical Accessories...

Страница 77: ...atic objectives the finest structures are visible with high contrast The sophisticated apochromatic correction allows these objectives to attain the highest color brilliance and fidelity Achromatic ob...

Страница 78: ...Leica M Series User Manual Bases 78 Bases...

Страница 79: ...ases 79 Transmitted light Base Leica TL BFDF Controls Extensionplateofthetransmitted lightbaseTLBFDF Button to toggle between bright field dark field Connector for cold light sources light conductor a...

Страница 80: ...cent objects featuring contrasting structures The object is directly illuminated from below and is seen in its natural colors against a bright background O O Turn the control as far as it will go tow...

Страница 81: ...User Manual Bases 81 Leica IsoPro Non motorized Controls Operating the XY stage IsoPro 1 To move the stage in X direction rotate the outer knob 2 To move the stage in Y direction rotate the inner con...

Страница 82: ...Leica M Series User Manual System Illumination 82 System Illumination...

Страница 83: ...e bottom end of the guide rods In this position you always have the same contrast with identical illumination This guar antees the reproducibility of an experiment Contact with the base If the optics...

Страница 84: ...keys to toggle between the illumination scenarios shown below Maximum brightness For temperature reasons it is not possible to switch on all nine LEDs simultaneously There fore to provide bright over...

Страница 85: ...utomatically toggle between them For additional information refer to the LAS X online help Using the keyboard O O Use the key to switch the illumination on or off O O Use the and keys to adjust the br...

Страница 86: ...Leica M Series User Manual Accessories 86 Accessories...

Страница 87: ...o five foot switches can be configured with a microscope system They can be programmed in LAS X to control focus illumination and indi vidual memory positions Leica Hand Wheel and Foot Switch The hand...

Страница 88: ...ith freely programmable rotary knobs and function buttons Controls The functions of the individual knobs and screen elements can be configured both directly on the Leica SmartTouch and using the Leica...

Страница 89: ...Leica M Series User Manual Dimensional Drawings 89 Dimensional Drawings...

Страница 90: ...ual Dimensional Drawings 90 Leica M125 C Leica M165 C 120 120 24 455 292 217 120 120 277 24 400 455 292 217 120 120 277 24 400 455 Leica M125 C Leica M165 C with incident light base and new focusing c...

Страница 91: ...eica M165 C Continued 496 5 91 431 118 187 154 300 50 mm 496 5 91 431 522 118 187 154 300 370 Y 50 mm Y 75 mm 496 5 91 431 522 118 187 154 300 370 Y 50 mm Y 75 mm Leica M125 C Leica M165 C with transm...

Страница 92: ...User Manual Dimensional Drawings 92 Leica LED5000 MCI 132 275 3 157 83 5 112 45 112 90 45 36 22 132 275 3 157 83 5 112 45 112 90 45 36 22 132 275 3 157 83 5 112 45 112 90 45 36 22 Leica LED5000 MCI di...

Страница 93: ...Leica M Series User Manual Dimensional Drawings 93 Leica LED5000 RL 132 132 160 37 160 37 58 mm Leica LED5000 RL dimensions in mm...

Страница 94: ...Leica M Series User Manual Dimensional Drawings 94 Leica LED5000 NVI Leica LED5000 NVI for high performance stereo microscopes dimensions in mm...

Страница 95: ...Leica M Series User Manual Dimensional Drawings 95 Leica MATS TPX 190 140 220 4 11 134 98 170 10 447 164 10 447 275...

Страница 96: ...Leica M Series User Manual Specifications 96 Specifications...

Страница 97: ...planapochromatic 28 8 mm 2 3 mm 7 3 120 max 453 lp mm 61 5 mm planapochromatic 31 5 mm 1 92 mm 7 8 160 max 525 lp mm 61 5 mm planapochromatic 29 5 mm 1 44 mm Maximum values based on optics combinatio...

Страница 98: ...from 1 000 V to 100 V Coded motorized function Zoom iris diaphragm objective nosepiece coded only Zoom iris diaphragm objective nosepiece coded only Zoom iris diaphragm objective nosepiece encoded an...

Страница 99: ...change 5 12 200 parfocal Fast magnification change 4 67 240 parfocal Fast magnification change 4 99 320 parfocal Binocular tubes ergonomics Apochr binocular and trinocular tubes with synchronized inte...

Страница 100: ...e Leica IsoPro XY stage manual Illuminators LED5000 RL LED5000 SLI LED5000 MCI LED5000 HDI LED5000 NVI LED5000 CXI cold light sources Misc accessories Photography video Digital image capture systems f...

Страница 101: ...lumination types Bright field Yes Dark field Yes Oblique light No Relief Contrast System RC No CCIC Constant Color Intensity Control No Internal shutter lamp control Yes Integrated filter holder No Co...

Страница 102: ...idity operating storage 10 90 RH non condensing Operating altitude maximum 0 2 000 m Storage altitude nA Pollution degree of intended environment 2 office lab environment Accessories Various Leica con...

Страница 103: ...Leica M Series User Manual Appendix 103 Appendix...

Страница 104: ...planachromatic and planapochromatic objectives of the MZ125 MZ16 are used on the MS5 MZ6 MZ75 or MZ95 NFOV Field number of the eyepiece Field numbers are printed on the eyepieces 10 21 16 14 25 9 5 4...

Страница 105: ...lting from careless or improper handling Contact address However if your instrument should no longer function properly contact your technician your Leica representative or Leica Microsystems Switzerla...

Страница 106: ...ents Some components are made of polymer or are polymer coated They are therefore pleasant and convenient to handle The use of unsuit able cleaning agents and techniques can damage polymers Permitted...

Страница 107: ...to modifications LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH CONNECT WITH US www leica microsystems com Leica Microsystems Schweiz AG Max Schmidheiny Strasse 201...

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