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Version 1.1, Revision B

Operation

Tip

Detailed descriptions for inserting the knife into the individual knife holders are provided in

(

→

P. 68 – 6. Optional Accessories)

5.7

Trimming the specimen

5.7.1 Trimming in manual operation

Warning

Wheel turn direction of electric coarse feed incorrectly selected.

Specimen damage.

• Always assure that you have selected the correct turn direction of the coarse feed wheel before

turning the wheel.

Caution

Rotating the handwheel and the coarse feed wheel at the same time.

Specimen damage.

• Do not turn the coarse feed wheel whilst rotating the handwheel.

Fig. 64

• Use the

TRIM/SECT

button to select the

TRIM

mode.

• Set the desired trimming thickness.

• Release the handwheel locking mechanisim and the handwheel brake

lever.

• In

TRIM

mode, use the coarse feed buttons or electronic coarse feed

wheel to move the specimen towards the knife/blade.

• Trim the specimen by turning the handwheel or the coarse feed wheel

• Using the

CUT MODE

button, select the

ROCK

operating mode and cut the

specimen with forward and backward motions of the handwheel.

• Terminate trimming when the desired sectioning surface and depth have

been reached.

Warning

When doing fast manual trimming and fingers are put between the specimen and the knife/blade after

the handwheel is released.

Operator can get cut and injured due to the turning of the handwheel when it is unlocked.

• Do not put fingers between the specimen and the knife/blade during trimming and sectioning.

Содержание 14 9AUTO00C1

Страница 1: ...UTOCUT Rotary Microtome Instructions for Use English Order No 14 0519 80101 Revision B Always keep this manual with the instrument Read carefully before working with the instrument Version 1 1 Revisio...

Страница 2: ......

Страница 3: ...ical details of the present Instructions for Use are not to be considered warranted characteristics of our products These are determined only by the contract provisions agreed between ourselves and ou...

Страница 4: ...t Components and Specifications 25 3 1 Overview instrument components 25 3 2 Instrument specifications 26 3 3 Technical Data 28 4 Setting up the instrument 30 4 1 Installation site requirements 30 4 2...

Страница 5: ...tine 66 6 Optional Accessories 68 6 1 Assembly for fixture for specimen clamps 68 6 1 1 Rigid fixture for specimen clamps 68 6 1 2 Directional fixture for specimen clamps 69 6 1 3 Fine directional fix...

Страница 6: ...4 1 Replacing the pressure plate 107 7 4 2 Mounting the clamping top onto the segment arc 108 7 5 The setting of the clamping system of the clamp mount on the base plate 108 8 Cleaning and Maintenanc...

Страница 7: ...the machine or something in its vicinity Tip Gives you tips to simplify your workflow Item numbers for numbering illustrations Fig 7 1 Numbers in red refer to item numbers in illustrations Manufacture...

Страница 8: ...nternational NSF and others Symbol for alternating current PE terminal Power on Power off The package contents are fragile and must be handled with care The package must be kept in a dry environment M...

Страница 9: ...ndicating the instrument serial number is attached at the back side of the instrument 1 3 Intended use The HistoCore AUTOCUT is a fully automatic motorized rotary microtome with a separate control pan...

Страница 10: ...rious injury of persons and or property damage including specimen damage Never remove or modify any protective devices of the instrument or any accessories Only service personnel qualified by Leica Bi...

Страница 11: ...touch it only at the lifting points described in the Instructions for Use and Unpacking Instructions base plate at the front and under the instrument on the back Never lift the instrument by the handw...

Страница 12: ...o avoid accidental falling off Warning Section waste tray falling down after it has been detached Injury of persons Work especially carefully when detaching the section waste tray and put it in a safe...

Страница 13: ...ion working with the instrument Serious injury of persons and or specimen damage can occur when specimen approaches knife blade due to inappropriate operator actions for example because object head ma...

Страница 14: ...lade holder was uninstalled from the microtome Serious injury of persons due to contact with extremely sharp knife blade Before uninstalling the knife blade holder from the microtome always make sure...

Страница 15: ...cutting edge facing upwards and never try to catch a falling knife Warning Uploading or unloading the specimen on the microtome without appropriate clothes and in an inappropriate manner Operator can...

Страница 16: ...ning if you recognize insufficient sections results Make sure that all prerequisites for appropriate sectioning are met Refer to the troubleshooting section of these Instructions for Use for further d...

Страница 17: ...hat you have centered the handle of the handwheel Warning When doing fast manual trimming and fingers are put between the specimen and the knife blade after the handwheel is released Operator can get...

Страница 18: ...otating the handwheel and the coarse feed wheel at the same time Specimen damage Do not turn the coarse feed wheel whilst rotating the handwheel Caution The feather key is lost when mounting the handw...

Страница 19: ...fast homing or moving to the memory position Caution The memory position is set too close to the knife blade Specimen impaired Make sure that the specimen does not touch the cutting edge of the knife...

Страница 20: ...irection during cleaning Serious injury of persons Always wipe the knife from the back of the knife to the cutting edge Warning Removing the parts from the drying chamber 65 C during cleaning of the k...

Страница 21: ...ctive devices Warning Protective devices or safety accessories provided by manufacturer have been removed or modified Serious injury of persons and or property damage including specimen damage Never r...

Страница 22: ...he motorized sectioning use the RUN STOP button or ENABLE button or the BRAKE button on the separate control panel P 49 Start and stop motorized sectioning Using the handwheel brake lever Fig 2 3 on t...

Страница 23: ...sectioning mode Activate the handwheel locking mechanism To center the handwheel lightly pull the handle Fig 4 1 outwards and swivel it into the center of the handwheel Fig 4 2 Release the handle and...

Страница 24: ...der E consists of a red foldaway handle Fig 8 1 To cover the cutting edge fold the safety guard handle upwards as illustrated in Fig 8 Tip The clamping levers on the two in one blade holder E are not...

Страница 25: ...tch 9 Socket for backlighting 2 Coarse feed wheel 10 Handwheel brake lever 3 Separate control panel 11 Object head with orientation and UCC 4 Microtome base 12 Handwheel 5 Blade holder base 13 Handwhe...

Страница 26: ...e for manual sectioning applications Smooth running hand wheel allows two manual sectioning modes rocking mode and conventional manual sectioning with full hand wheel rotation and 3 motorized sectioni...

Страница 27: ...t distance without the need of deactivating the retraction Each change in the sense of rotation will be electronically detected and automatically converted into an advance or retraction movement of th...

Страница 28: ...humidity 20 to max 80 non condensing Operating altitude Up to 2000 m above sea level Transport temperature range 29 C to 50 C Storage temperature range 5 C to 50 C Transport and storage relative humi...

Страница 29: ...n orientation Maximum sectioning area with retraction 60 mm Maximum specimen block size for large standard clamp H x W x D Maximum specimen block size for super mega cassette clamp H x W x D 55 x 50 x...

Страница 30: ...he instrument in rooms with an explosive hazard 4 2 Standard delivery packing list The HistoCore AUTOCUT is available in two configurations HistoCore AUTOCUT configuration 14 9AUTO00C1 Quantity Part d...

Страница 31: ...ife holder set up and waste tray select one Specimen orientation 14050237717 Fine directional fixture for specimen clamp 14050238949 Directional fixture for specimen clamp 14050238160 Rigid fixture fo...

Страница 32: ...trument Once unpacked the instrument must be moved only in an upright position Thoroughly follow the instructions given in the Unpacking Instructions attached at the outside of the package or the acti...

Страница 33: ...ositioning Caution Accessories components loosening damaged during transportation Property damage or delayed diagnosis The packaging has two indicators ShockDot Impact Indicator and Tilt Indicator tha...

Страница 34: ...uter carton wall Fig 13 7 Lift the instrument Fig 13 8 by holding it by the base plate at the front Fig 14 11 and under the instrument on the back Fig 14 12 and lift it out of the formed cushion Fig 1...

Страница 35: ...and it may cause the delay of diagnosis Before assembling the handwheel check the feather key and make sure that it is in the handwheel shaft 2 Place the spacer Fig 15 4 and the handwheel Fig 15 5 on...

Страница 36: ...ove the main power switch on the left rear side of the instrument The voltage setting is displayed in the viewing window Fig 16 1 1 Insert a small screwdriver into the cutout Fig 16 3 and carefully pr...

Страница 37: ...1 3 4 If a foot switch is to be used with the instrument insert the foot switch cable Fig 18 1 into the connecting jack Fig 18 2 on the rear of the instrument and fasten it by tightening the screws Fi...

Страница 38: ...nsporting the instrument wait at least two hours to allow the instrument to adopt the ambient temperature before turning it on Warning Misinterpretation of the yellow LED in the M STOP field Serious i...

Страница 39: ...which setting was last activated This is indicated simultaneously on the control panel and microtome The LED of the active mode here the section thickness is illuminated in green When the red LED in t...

Страница 40: ...e freeze Specimen damage and or delay of diagnose In case of a software freeze follow the instructions given in chapters Troubleshooting and Operation If instrument cannot set back into operation imme...

Страница 41: ...tion thickness sum to 0 12 MENUMODE CLEAR Pressing two buttons simultaneously switches to the setting of the retraction value 5 1 2 Separate control panel Fig 21 1 9 5 3 11 7 2 10 6 4 12 8 13 17 15 19...

Страница 42: ...MODE button Selects cutting mode 14 Button of setting sectioning window Sets sectioning window The green LED flashes until second sectioning window edge is set 15 MEMO button Sets one memory position...

Страница 43: ...ion thickness setting range 0 50 m 100 m Setting values from 0 5 m 5 0 m in 0 5 m increments from 5 0 m 20 0 m in 1 0 m increments from 20 0 m 60 0 m in 5 0 m increments from 60 0 m 100 0 m in 10 0 m...

Страница 44: ...coarse feed buttons have the same functions as in the trimming mode The STEP function is useful for careful step by step approximation of the specimen towards the knife How to activate the STEP functi...

Страница 45: ...osition Fig 25 1 2 3 4 5 Set one memory position using the MEMO button Fig 25 1 on the separate control panel To set one memory position move the object head to the desired position and press the MEMO...

Страница 46: ...ot turn the handwheel or press any button on the separate control panel or the instrument control panel during fast homing or moving to the memory position Caution The memory position is set too close...

Страница 47: ...position of the object head In motorized sectioning mode the retraction depends on the setting of the sectioning speed In manual mode the retraction value can be selected in 5 m increments between 5 a...

Страница 48: ...poses and does not indicate a particular speed Sectioning window setting Fig 30 1 The sectioning window function allows to optimally adapt the size of the sectioning area to the actual size of the spe...

Страница 49: ...ing mode Turn the handwheel a short distance forwards and backwards for sectioning rocking mode Each change in the sense of rotation will be electronically detected and automatically converted into an...

Страница 50: ...the electronic brake function does not work If the BRAKE button is pressed during manual sectioning a warning sound is heard Caution Using the electronic brake to lock the handwheel People injury Dam...

Страница 51: ...ights up from the beginning of the remaining feed In addition an acoustic signal is heard for approx 2 seconds The sectioning process is interrupted and the object head stops in the upper end position...

Страница 52: ...rating mode Press the foot switch once briefly to start motorized sectioning Press the foot switch again to stop it The specimen stops in the next upper end position Fig 45 SINGLE single stroke operat...

Страница 53: ...d switch the instrument on while holding the slow forward coarse feed button Fig 49 1 on the separate control panel three digit display indicates C for clockwise Fig 49 2 To set counterclockwise rotat...

Страница 54: ...t not be oriented during the retraction phase If a block is oriented during retraction the block will be fed by the retraction value plus the selected section thickness before the next section This ma...

Страница 55: ...amp the specimen Set the object head to half the height of the vertical travel range by turning the handwheel Fig 51 If the object head remains in this exact position the setting is correct If the obj...

Страница 56: ...bject head moves upwards turn the screw Fig 52 1 approx 1 2 turn counterclockwise each time Continue this procedure until the object head no longer moves once released Tip Repeat the adjusting procedu...

Страница 57: ...he two in one blade holder E Fig 54 8 with the underside groove onto the T piece Fig 54 7 of the knife holder base Fig 53 2 3 To clamp retighten the eccentric bolt Fig 54 9 5 3 Adjusting the clearance...

Страница 58: ...the specimen is clamped into place You can use the quick clamping system Fig 56 5 to hold all available accessory specimen clamps for more information see P 68 6 Optional Accessories To do so proceed...

Страница 59: ...s provided in P 68 6 Optional Accessories 5 6 Clamping the knife disposable blade Warning Knives or blades are handled and or disposed in an inappropriate manner Serious injury of persons due to conta...

Страница 60: ...ig 60 1 When using low profile blades the insert Fig 60 1 for low profile blades must first be placed in the two in one blade holder E and make sure that the bottom edge of the insert fits closely to...

Страница 61: ...ser Fig 63 Fig 63 Warning Knives or blades are handled and or disposed in an inappropriate manner Serious injury of persons due to contact with extremely sharp knives and or blades Work especially tho...

Страница 62: ...TRIM mode Set the desired trimming thickness Release the handwheel locking mechanisim and the handwheel brake lever In TRIM mode use the coarse feed buttons or electronic coarse feed wheel to move th...

Страница 63: ...the handle of the handwheel Warning Inappropriate selection of sectioning speed Specimen damage Always set the sectioning speed according to the hardness of the specimen For hard specimens always sele...

Страница 64: ...mask and safety goggles Warning Working with the instrument and brittle specimen without appropriate protective clothes Serious injury of persons by splinters while cutting brittle specimen Always wea...

Страница 65: ...f trimming with the electronic coarse feed remember to switch back to sectioning mode Before starting sectioning always make sure that an appropriate sectioning thickness has been selected Fig 66 Alwa...

Страница 66: ...men clamp prior to changing the specimen and during all work breaks Warning Uploading or unloading the specimen on the microtome without appropriate clothes and in an inappropriate manner Operator can...

Страница 67: ...g knife Warning Paraffin waste falls on the floor and is not cleaned up Serious injury of persons for example by slipping and stumbling into the knife blade Always clean up paraffin waste before it sp...

Страница 68: ...r specimen clamps Before assembling the fixture for specimen clamps activate the handwheel locking mechanism 6 1 1 Rigid fixture for specimen clamps Tips Remove the rubber ring Fig 68 5 only after att...

Страница 69: ...the bores as shown and screw them in using an Allen key No 3 Insert the spring Fig 69 3 and pin Fig 69 4 with the flatter side into the thrust piece Fig 69 2 Completely screw in the thrust piece with...

Страница 70: ...rectional fixture for specimen clamps with zero point indicators or the directional fixture for specimen clamps Screw the 4 screws Fig 71 2 into bore Fig 71 1 with an Allen key size 2 5 and tighten th...

Страница 71: ...ble jaw upward against the fixed jaw to securely clamp the sample Warning Insufficient frame conditions for sectioning Specimen damage or poor sectioning results for example sections alternating in th...

Страница 72: ...e V insert upward against the fixed jaw to securely clamp the sample 6 2 3 Foil clamp type 1 The foil clamp type 1 is appropriate both for clamping very small and thin foil pieces and flat angular spe...

Страница 73: ...the standard specimen clamp as shown Turn the knurled screw Fig 75 6 clockwise to clamp the foil clamp in the standard specimen clamp 6 2 4 Universal cassette clamp Warning Insufficient frame conditi...

Страница 74: ...cessary the specimen must be clamped horizontally Caution When using thin walled cassettes the cassettes become deformed or insecurely clamped into place or other problems because of the clamping syst...

Страница 75: ...he left into the dovetail guide of the rigid fixture for specimen clamps and tighten screw Fig 77 1 Caution When using super mega cassette clamp the orientation is not set to position 0 when direction...

Страница 76: ...9 To orient the inserted specimen insert the pin Fig 78 7 into the bore Fig 78 8 and rotate it counterclockwise to release the clamp You can now rotate the specimen so that the side you want faces upw...

Страница 77: ...kwise 6 3 2 Knife holder E TC Warning The knife blade is installed before the knife blade holder and the knife blade holder base are installed on the instrument Serious injury of persons due to contac...

Страница 78: ...low profile disposable blades The two in one blade holder E has a lateral displacement function so that the entire width of the blade can be used It is equipped with a blade ejector to safely remove t...

Страница 79: ...otate the lever Fig 83 1 clockwise 6 3 4 Knife holder E with a water trough for low profile blades Fig 84 3 1 2 The knife holder E with a water trough Fig 84 is only for low profile blades The safety...

Страница 80: ...ide knives profile c and d up to 16 cm long The integrated height adjustment feature allows you to also use knives that have been resharpened numerous times Knife holder N Fig 86 For holding conventio...

Страница 81: ...ig 88 1 can be inserted whereas the shining facet of profile d knife Fig 88 2 should face the operator Putting the knife wrongly will damage the specimen and the knife Knife height adjustment When adj...

Страница 82: ...s ease of cleaning due to the anti static surface Push the section waste tray Fig 89 1 from the front to the microtome base plate Fig 89 2 until it is held in place by the two magnets Fig 89 3 on the...

Страница 83: ...acklighting illumination must be detached NEVER use the super mega cassette clamp with backlighting illumination The force balance system has to be adjusted when using the super mega cassette clamp 6...

Страница 84: ...5 Fig 93 4 Support plate large for BIOCUT MULTICUT and AUTOCUT Fig 93 5 Support plate small for NANOCUT Fig 93 6 Allen key No 3 Fig 93 7 4 countersunk screws to install support plate Fig 93 8 Allen ke...

Страница 85: ...n Fig 96 2 onto the baseplate from above and tighten with the Allen key size 8 Warning The universal microscope carrier tips Serious injury of persons After installing the vertical column immediately...

Страница 86: ...oscope magnifying lens or cold light source please see the appropriate Instructions for Use 6 8 Magnifying lens LED illumination Tips The magnifying lens provides a 2x magnification and can be used wi...

Страница 87: ...adjust the brightness of the spot lights Fig 99 5 1 6 7 2 3 4 8 9 Adjust the position of the magnifying lens to the specimen being processed The magnifying lens can be swiveled completely to the side...

Страница 88: ...ventional knives up to 16 cm in length Height adjustment of the knife Separate clearance angle adjustment Movable safety guard Order No 14 0502 37993 Knife holder NZ silver Fig 102 For holding convent...

Страница 89: ...3 click stop positions make it easier to utilize the entire blade length Color coded foldaway safety guard Order No 14 0502 38961 Knife holder E TC Fig 104 For tungsten carbide disposable blades TC 65...

Страница 90: ...e it easier to utilize the entire blade length The pressure plate is replaceable Color coded foldaway safety guard With blade ejector Order No 14 0502 54497 Disposable blades low profile 819 Fig 107 8...

Страница 91: ...rbide metal guarantees sections to approx 2 m The blades are fully recyclable Length 65 mm Thickness 1 mm Height 11 mm 1 pack of 5 pcs Order No 14 0216 26379 Knife 16 cm profile c steel Knife 16 cm pr...

Страница 92: ...Order No 14 0216 04206 Fig 112 1 is the carbide metal knife serial number Knife case Fig 113 Variable knife case plastic for 1 or 2 knives 10 16 cm long Order No 14 0213 11140 Universal cassette clam...

Страница 93: ...e directional Fig 117 Silver for holding the standard clamp universal cassette clamp round specimen holder and EM specimen holder including installation hardware Note Quick clamping system 14 0502 377...

Страница 94: ...18 must be ordered separately Order No 14 0502 37717 Quick clamping system Fig 120 For specimen holder for use with the fine directional fixture for specimen clamps with zero point indicators 14 0502...

Страница 95: ...ecimen clamps 14 0502 38005 and 14 0502 37998 black Maximum specimen size 25 x 13 mm Order No 14 0402 09307 EM specimen holder fixture Fig 124 For EM specimen holder black For samples with a 10 mm dia...

Страница 96: ...er mega cassette clamp Fig 127 With adapter silver Note Use only together with non orientable specimen holder fixture 14 0502 38160 which has to be ordered separately Backlighting 14 0502 38719 cannot...

Страница 97: ...men clamps to be changed quickly and reliably without modifying the handwheel Antistatic material makes cleaning easier Can be retrofitted for all rotary microtomes from HistoCore series Technical dat...

Страница 98: ...nification Note Magnifier includes an adapter for holding the optional LED illumination Order No 14 0502 42790 Universal microscope carrier Fig 130 Universal assembly Order No 14 0502 40580 Backlighti...

Страница 99: ...series Order No 14 0517 56261 Section waste tray Fig 133 For HistoCore rotary microtome series Anti static section waste tray Order No 14 0517 56237 Section waste tray Order No 14 0518 56458 Brush Fig...

Страница 100: ...high profile 1 pack of 50 80 x 14 x 0 35 mm 14 0358 38926 Disposable blades high profile 10 packs of 50 14 0358 38383 Disposable tungsten carbide blades TC 65 14 0216 26379 Knife 16 cm profile c stee...

Страница 101: ...unction with LED 1000 control unit Order No 14 6000 04825 14 6000 04826 LED 1000 control unit This module can only function with LED 1000 Hi power spots 2 arm Order No 14 6000 04826 14 6000 04825 Back...

Страница 102: ...instrument and check if error code E2 still exists 2 If error code E2 still exists call Leica Biosystems service Error code E3 is displayed a two second long warning beep is heard The instrument does...

Страница 103: ...amped in the universal cassette clamp If the universal cassette clamp is soiled by paraffin clean the universal cassette clamp P 109 8 1 Cleaning the instrument When using cassettes whose lid is molde...

Страница 104: ...mped into place an alternative tensioning clamp must be used Laterally displace the knife blade holder or insert a new knife blade Insert a new pressure plate or use a new two in one blade holder Read...

Страница 105: ...ed and or section thickness when trimming Select a smaller section thickness rotate the handwheel more slowly Adjust the clearance angle 7 3 Instrument malfunctions The following is a list of the most...

Страница 106: ...ment was switched on Select the trimming mode by pressing TRIM SECT Then retract the specimen using the coarse feed button See above The coarse feed buttons can be used to move the object in a backwar...

Страница 107: ...the clamping lever counterclockwise Fig 137 2 3 Pull out the bolt Fig 137 3 Fig 138 3 4 Remove the damaged pressure plate Fig 138 4 Tips Make sure that the insert of the clamping top is installed as...

Страница 108: ...a 2 5 mm Allen key at the setscrew Fig 139 1 so that the lever initially slips when the lever is activated Continue turning the setscrew Fig 139 1 a little bit approx 1 4 turn to the left or right th...

Страница 109: ...of persons due to contact with extremely sharp knife blade Before uninstalling the knife blade holder from the microtome always make sure to detach the knife blade wearing cut resistant gloves and st...

Страница 110: ...and activate the handwheel lock Switch the instrument off and unplug it Remove the blade from the blade holder and insert it in the receptacle at the bottom of the dispenser or remove the knife from t...

Страница 111: ...141 7 and take the pressure plate off 3 Remove the clamping top To do so rotate the clamping lever Fig 141 2 of the lateral displacement counterclockwise and pull it out sideways push the clamping top...

Страница 112: ...e of the clamping top Fig 141 3 Tips If the insert of the clamping top is installed incorrectly the pressure plate cannot be clamped Universal cassette clamp Fig 142 1 2 3 Detach cassette clamp Fig 14...

Страница 113: ...e internal components of the instrument for service and repair The instrument is basically maintenance free To ensure trouble free operation of the instrument over a long period of time the following...

Страница 114: ...tems service technician regardless of how heavily the instrument has been used 8 4 Lubricating the instrument Once a month lubricate the following parts with the included drive parts oil 1 2 drops are...

Страница 115: ...e holder E Fig 146 1 2 Clamping lever Fig 146 1 for the lateral displacement Clamping lever Fig 146 2 for the blade Universal cassette clamp Fig 147 1 2 Lubricate the spiral springs Fig 147 1 of the t...

Страница 116: ...ty terms of your Leica Biosystems sales organization or the organization from which you have purchased the contractual product shall apply exclusively 9 2 Service information If you are in need of tec...

Страница 117: ...LeicaBiosystems com within the product menu This template has to be used for gathering all required data When returning a product a copy of the filled and signed confirmation has to be enclosed or pa...

Страница 118: ...118 Version 1 1 Revision B Notes...

Страница 119: ......

Страница 120: ...w LeicaBiosystems com Version 1 1 Revision B 10 2018 Leica Biosystems Nussloch GmbH Heidelberger Str 17 19 D 69226 Nussloch Germany Tel 49 0 6224 143 0 Fax 49 0 6224 143 268 Web www LeicaBiosystems co...

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