6. Application Example
53
Design Layout
Define the plate layout using the
Well Assignment
dialog box on the right side of
the screen.
In the
Identifiers
group box, select
BL (Blank)
.
In the
Experimental
group
box the number
1
remains.
In the
Replicates
group box,
All
is selected automatically.
Click well
A1
, which is then marked with a red border.
Click
Fill selection
and the well is labeled with the selected identifier type.
Note
A single well can also be filled by double-clicking it.
Now choose the following settings in the
Well Assignment
dialog box:
In the
Identifiers
group box, select
NC
(Negative Control).
In the
Experimental
group box the number
1
remains.
All
in the
Replicates
group box is selected automatically.
Starting at well
B1
click and drag the mouse to
C1
. The wells
B1
to
C1
are then
marked with a red border.
Click
Fill selection
and the wells are labeled with the selected identifier type.
Calibrators (standards) must be assigned to wells
D1
to
G2
. Select the following
settings in the
Well Assignment
dialog box:
In the
Identifiers
group
box, select
ST (Standard)
.
In the
Experimental
group
box the number
1
remains.
In the
Replicates
group box, choose between
Fix number
and
All
.
Fix number:
Only enabled for standards and samples where IDs can be used.
If this
Fix number
button is active a number can be entered in the corresponding
text field. This number defines how many replicates are intended for this method.
In the selected wells, the entered number of replicates for every ID is created.
Therefore the number of selected wells must be a multiple of the entered number
of replicates.
