15
6. Release the pressure bars and gently pry apart the glass plates. The gel will
usually stick to one of the plates and can be removed by first soaking in running
buffer and then gently lifting with a spatula.
7. The gel is now ready to be stained with Coomassie or silver stain for specific
band identification and further analysis. If using the Enduro GDS for imaging you
will need the white light conversion screen.
Table 7.
Recommended Voltage and Current settings
for 1mm thick, 12% gels.
VE10
One gel
90-225V,
20-45mA
Two gels
90-225V,
40-90mA
Three gels
90-225V,
60-135mA
Four gels
90-225V,
80-180mA
The recommended power condition for optimal resolution with minimal thermal band
distortion is 150 volts, constant voltage setting. No adjustment of the setting is
necessary for thickness or number of gels. The usual run time is approximately 40-
45 minutes. Current should be approximately 50mA per gel (120mA for two gels) at
the beginning of the run. During the 45 minute run, the current will slowly drop to
about 20mA per gel. This drop is caused by the change in buffer ions in the gel,
causing a slow rise in the resistance in the gel. As one would expect from the Ohms
law (V=I*R), at constant voltage (V) a rise in the resistance (R) results in a drop in
the current (I).
