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Chapter 3: Getting Started

Product Service 1-800-522-7658

13

Installation of Gloves to the Glove Ports

With the thumbs up and in a right/left orientation, secure the gloves in place
on the glove ports by stretching the beaded glove cuff into the glove port
groove nearest the window. Install the separate 8" diameter O-ring over the
gloves, into the outer groove of the glove port surface. Stainless steel band
clamps are provided for securing the separate O-ring into the glove port
groove. Replacement gloves and parts are listed in Chapter 7.

Figure 3-4

Glove Installation

Connecting the Electrical Supply Source to the
Glove Box

115V, 60 Hz; 100V, 50 Hz; or 100V, 60 Hz Models

Connect the power cord supplied to the IEC electrical supply plug located on
the back of the glove box. 115V or 100V models are rated at 8 amps total.

The maximum circuit load for the interior electrical duplex is 5 amps.

230V, 50 Hz or 230V, 60 Hz Models

The same procedure applies for the 230V international models except the
cords are shipped without an attached plug. Install the appropriate plug for
your electrical specifications per local codes. The 230V, 50 Hz or 230V, 60
Hz models are both rated at 4 amps total.

The interior electrical duplex is

rated at 2 amps.

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Содержание Precise 5220100

Страница 1: ...love Boxes CA Models Voltage Frequency Basic Models Voltage Frequency 5220100 115V 60 Hz 5220000 115V 60 Hz 5220120 230V 50 Hz 5220020 230V 50 Hz 5220121 230V 60 Hz 5220021 230V 60 Hz 5220130 100V 50 Hz 5220030 100V 50 Hz 5220131 100V 60 Hz 5220031 100V 60 Hz ...

Страница 2: ...p m Central Standard Time Part 5233701 Rev E ECO L353 Warranty Labconco Corporation provides a warranty to the original buyer for the repair or replacement of parts and reasonable labor as a result of normal and proper use of the equipment with compatible chemicals Broken glassware and maintenance items such as filters gaskets light bulbs finishes and lubrication are not warranted Excluded from wa...

Страница 3: ...e Ports 13 Connecting the Electrical Supply Source 13 Connect the Plumbing Lines for Gas Vacuum Drying Train 14 Validating the Glove Box 20 Oxygen Method Leak Testing Summary ISO 10648 2 22 Pressure Change Method Leak Testing Summary ISO 10648 2 23 CHAPTER 4 PERFORMANCE FEATURES AND SAFETY PRECAUTIONS 24 Performance Features 24 Safety Precautions 27 CHAPTER 5 USING YOUR GLOVE BOX 29 Routine Daily ...

Страница 4: ...IX C SPECIFICATIONS 47 APPENDIX D PRECISE CONTROLLED ATMOSPHERE PURGE FILL REDUCTION RATES 48 APPENDIX E LIFE SPAN FOR OXYGEN REMOVAL COLUMN MOISTURE TRAP REMOVAL 50 APPENDIX F IONIZER FAN PERFORMANCE CRITERIA 52 APPENDIX G HELIUM LEAK TEST METHOD 54 APPENDIX H PLUMBING DIAMGRAMS 56 APPENDIX I PRESSURE FLOW RATE CONVERSIONS 58 APPENDIX J REFERENCES 59 ...

Страница 5: ...dous materials Second it protects materials and laboratory equipment inside the main chamber from the effects of exposure to ambient air oxygen and moisture levels Third it provides a protective leak tight environment for your research testing or production procedures the leak rate of the glove box is rated Class 1 for controlled atmosphere chambers per ISO 10648 2 test methods Each Precise Contro...

Страница 6: ...b to provide efficient workflow and prevent operator interference from normal traffic flow Carefully read this chapter to learn the requirements for your installation site The support vibration and preventive requirements The location requirements The exhaust requirements for the pressure relief valve The electrical power requirements The plumbing requirements for gas vacuum and dry train The spac...

Страница 7: ...ements The Precise Glove Boxes have been designed to rest on a typical 29 30 737mm 762mm deep work surface The height should be 35 40 for standing position Avoid placing the glove box in high traffic areas where walking might disrupt the operator or experimentation Exhaust Requirements for the Pressure Relief Valve To prevent over or under pressurization of the main glove box chamber the Precise C...

Страница 8: ...re explosive hazard or damage to property inside the glove box See Figures 3 1 3 2 and 3 3 in Chapter 3 for ventilation connection diagrams of the vent kits Electrical Requirements Electrical receptacles should be located nearby for connecting the glove box or other optional laboratory equipment The entire glove box is rated 115V 8A or 230V 4A or 100V 8A One duplex outlet is located inside the glo...

Страница 9: ...ccessory Pressure Controller and Chapter 7 for ordering information The Pressure Controller automatically monitors and controls pressures in both the main chamber and transfer chamber it also automates the operation of purging and filling each chamber gas outlet and gas inlet up to 199 cycles Consult your Safety Officer should you require ventilation and or filtration of the vacuum pump exhaust Fi...

Страница 10: ...ructure and work surface Connect an exhaust system to the pressure relief valve if applicable Install the gloves Connect the electrical supply Connect the plumbing lines for gas vacuum and drying train Validate performance of the glove box Each Precise Glove Box model weighs 185 lbs each 83 kg The shipping container allows for lifting with a mechanical lift truck or floor jack If you must lift the...

Страница 11: ... using a floor jack Carefully remove the shrink wrap and carton on the glove box and inspect it for damage that may have occurred in transit If damaged notify the delivery carrier immediately and retain the entire shipment intact for inspection by the carrier THE UNITED STATES INTERSTATE COMMERCE COMMISSION RULES REQUIRE THAT CLAIMS BE FILED WITH THE DELIVERY CARRIER WITHIN FIFTEEN 15 DAYS OF DELI...

Страница 12: ...for optimal comfort A height of 35 40 889mm 1016mm is appropriate for standing operation Labconco base stands from Chapter 7 may be adjusted in height from 33 40 838mm 1016mm Connecting the Exhaust to the Pressure Relief Valve If Applicable WARNING The weight of the pressure relief exhaust ductwork system must be supported independently of the glove box superstructure or damage may occur The exhau...

Страница 13: ...connections See Chapter 7 Accessories to add a pressure relief valve 5230600 to the Precise Basic Glove Box A 2 06 52 3mm diameter hole must be drilled into the Precise Basic Glove Box shell This hole is located on the 4 00 101 6mm diameter x 1 00 25 4mm high raised boss the raised boss is located on the top of the glove box shell in the front right side corner Install the pressure relief valve fr...

Страница 14: ...Chapter 3 Getting Started Product Service 1 800 522 7658 10 Figure 3 1 Pressure Relief Vent Kit 5242700 ...

Страница 15: ...Chapter 3 Getting Started Product Service 1 800 522 7658 11 Figure 3 2 HEPA Filtered Pressure Relief Vent Kit 5238500 ...

Страница 16: ...Chapter 3 Getting Started Product Service 1 800 522 7658 12 Figure 3 3 Carbon Filtered Pressure Relief Vent Kit 5241200 ...

Страница 17: ...are listed in Chapter 7 Figure 3 4 Glove Installation Connecting the Electrical Supply Source to the Glove Box 115V 60 Hz 100V 50 Hz or 100V 60 Hz Models Connect the power cord supplied to the IEC electrical supply plug located on the back of the glove box 115V or 100V models are rated at 8 amps total The maximum circuit load for the interior electrical duplex is 5 amps 230V 50 Hz or 230V 60 Hz Mo...

Страница 18: ... a tube support insert See Figure 3 5 A and B for both methods Consult Figure 3 8 which depicts typical tubing connections with the accessory Automatic Pressure Controller Vacuum pump model numbers can be found in Chapter 7 Consult your Safety Officer to determine the suitability and requirement for venting the exhaust port of a vacuum pump to the outside An accessory 0 2 micron filter can be inst...

Страница 19: ...gure 3 5 Close up View of Typical Valve Connection Options Alternative Method with Hose Connector Primary Method for Tygon Tubing Hose Connector Small Ferrule Compression Nut Compression Nut Small Ferrule Large Ferrule Tube Insert Note Hose Clamp not supplied B A ...

Страница 20: ...Chapter 3 Getting Started Product Service 1 800 522 7658 16 Figure 3 6 Typical Tubing Connections for Manual Valve Operation Tubing Connection Kit 5245100 provides full details ...

Страница 21: ...Chapter 3 Getting Started Product Service 1 800 522 7658 17 Figure 3 7 Typical Tubing Connections with Automatic Pressure Controller Tubing Connection Kit 5245200 provides full details ...

Страница 22: ...apter 3 Getting Started Product Service 1 800 522 7658 18 Figure 3 8 Typical Connections Shown with Connection to the Automatic Pressure Controller Full details provided with Automatic Pressure Controller ...

Страница 23: ...Getting Started Product Service 1 800 522 7658 19 Figure 3 9 Typical Tubing Connections for Oxygen Moisture Removal Shown with the Automatic Pressure Controller Tubing Connection Kit 5242501 provides full details ...

Страница 24: ...s greater than 1 x 10 4 cc sec while at a positive pressure of 5 inches helium Refer to Chapter 6 Initial Certification and Appendix G for Helium Leak Test Method Overall Glove Box Main Chamber Leak Test The Precise Controlled Atmosphere Glove Box has been tested with an oxygen monitor detecting oxygen levels as low as 1 ppm the rate of increase in oxygen over time was determined in accordance wit...

Страница 25: ...4 Class 1 Leak Rate 5 x 10 4 Results were attained at Labconco Corporation under controlled conditions Actual results may vary Figure 3 11 The validation requirements and required testing instruments for all applications shall be determined by the end user and Safety Officer Other validation test methods such as pressure leak decay tests may be conducted per ISO 10648 2 standards The pressure leak...

Страница 26: ... classification of leak tightness of each glove box must be decided by the end user their Safety Officer and any oversight authorities Oxygen Method Leak Testing Summary ISO 10648 2 The method consists of measuring the increase in the oxygen concentration as a function of time inside the glove box main chamber previously purged with an inert gas The difference in the oxygen concentration between t...

Страница 27: ... adjustments for barometric pressure and temperature changes which can be calculated as Tf Tf 60 t PnT1 P1Tn 1 where t is the duration of the test in minutes P1 is the absolute pressure ambient barometric pressure minus under pressure at the first reading in Pascals Pn is the absolute pressure ambient barometric pressure minus under pressure at the last reading in Pascals T1 is the temperature at ...

Страница 28: ...h procedures The Precise Basic Glove Box allows the end user to customize the glove box or use the glove box in its simplest form See Figure 4 1 to refer to a detailed description of the Precise Controlled Atmosphere Glove Box main performance features 1 Molded One Piece Chemically Resistant Glove Box Shell made of medium density polyethylene which provides superior chemical resistance The molded ...

Страница 29: ... reads from 5 to 5 inches of water pressure Note The Main Chamber Pressure Gauge is included on Precise Controlled Atmosphere Glove Boxes and can be ordered from Chapter 7 for modification of Precise Basic Glove Boxes 5 Glove Ports are 8 nominal size and molded into the main glove box shell The double grooves provide two points of glove restraint for containment and safe changing of the gloves 6 N...

Страница 30: ...save space IMPORTANT The transfer chamber must have an optional High Vacuum Sleeve if the chamber is converted for vacuum operation on Precise Basic Glove Boxes See Chapter 7 13 Pressure Relief Valve provides a safety relief for over or under pressurization events within the glove box main chamber The valve is set to relieve positive or negative pressures above 6 or below 6 inches of water gauge T...

Страница 31: ...icals 5 The use of flammable gases or solvents in the glove box should be limited Care must be taken to ensure against the concentration buildup of flammable or explosive gases or vapors Use of an open flame or exposure to high heat should be avoided in the glove box as the heat will damage the liner DANGER Use of added oxygen inside the glove box must be avoided to prevent explosion death or seri...

Страница 32: ...y officer Venting or filtering of vacuum pump exhaust is the operator s responsibility 14 Ensure that the glove box is connected to electrical service in accordance with local and national electrical codes Failure to do so may create a fire or electrical hazard Do not remove or service any electrical components without first disconnecting the glove box from electrical service 15 Ensure only traine...

Страница 33: ... beginning work Work from a written plan Arrange for minimal disruptions while the glove box is in use Consult your Safety Officer for personal protective equipment recommendations and operating pressure confirmation Start up Basic start up procedure includes turning on the vacuum pump opening the inert gas valve on the gas supply tank and turning on the glove box light Loading Materials and Dryin...

Страница 34: ... oxygen levels when the glove box is not in use Maintaining a slight main chamber positive pressure can help to preserve the glove box atmosphere The moisture oxygen permeation rates are reduced at slightly positive pressures see Appendix E Recirculating drying trains are required to further reduce and maintain oxygen and moisture levels below 1 Labconco oxygen and moisture removal drying trains c...

Страница 35: ... controlled atmosphere leave the glove box main chamber under slightly positive pressure and continuously run the recirculating drying train The use of the accessory external glove port caps can assist in preserving the inert atmosphere see Appendix E Keep the outer door closed when not in use Hazardous Use with Chemicals Hazardous chemicals may be used safely only when the glove box vacuum pump a...

Страница 36: ... with a disinfectant or cleaner depending upon the application Using an appropriate cleaner and cloth clean the exterior surfaces of the glove box particularly the front and top to remove any accumulated dust Chart the pressure decay of the glove box noting the change in pressure over short time periods Adjust as necessary for temperature and barometric pressure changes See Chapter 3 Validating th...

Страница 37: ... 2 test methods Pressure Change Leak Test per ISO 10648 2 test methods Fluorescent Light Replacement 1 Disconnect the power Refer to Appendix A for replacement parts 2 Locate the light reflector on the top of the glove box behind the front panel 3 Remove the light reflector support by removing two hex nuts located on both sides of the light reflector 4 Remove the old fluorescent lamp 5 Reinstall t...

Страница 38: ...ugh so the top of the flat washer is flush with plastic body of the glove box then replace glass and frame Again tighten screws with minor turns and across from each other uniformly diagonally to apply pressure evenly Use thread lock to keep screws secure Double sided tape should be OK not needed when just replacing glass ITEM PART NO DESCRIPTION 1 5227600 Standoff 50 Hex Female 2 5226700 Frame Wi...

Страница 39: ... 9 Refer to Appendix A Appendix B and Chapters 3 and 7 for installation diagrams Service Valve and Pressure Gauge Replacement 1 Decontaminate the glove box interior if required 2 Wear appropriate protective equipment as determined by your Safety Officer 3 Turn the glove box and vacuum pump OFF 4 Remove the valve or pressure gauge by loosening the large nut for the valve or hardware for the pressur...

Страница 40: ...ave an adjustable height range from 33 to 40 838mm 1 016mm and include a lower shelf for supporting accessory equipment 4 Anti Static Ionizer Fan Part 5234400 100 115V or Part 5234401 230V International Used for weighing operations and attaches to the ceiling of the glove box The ionizer fan circulates ionized air inside the glove box to reduce static charge Ionizer Replacement Points set of 8 emi...

Страница 41: ...0400 European Power Strip 250V 4 Position 2A 5332600 Australian Power Strip 250V 6 Position 2A 5211501 Installation Tool 9 Electrical 9 Pin Sealed Pass Through Kit Part 5075619 Includes sealed 9 pin D subminiature with male pins on one side and female pins on the other connector and cord for data transfer to a typical printer outside the glove box from the balance Note Order the 9 pin installation...

Страница 42: ...e Relief Vent Kit No Filters Outside 5238500 HEPA Filtered Pressure Relief Vent Kit Room or Outside 5241200 Carbon Filtered Pressure Relief Vent Kit Room or Outside 5230600 Pressure Relief Valve Basic add on All Vent Kits 5238500 and 5241200 may be used together if required 12 Gas Inlet Vacuum Outlet Tubing Kits and Direct Drive Vacuum Pumps These flexible vinyl tubing kits provide the necessary t...

Страница 43: ...ber and transfer chamber The end user can also automate the purge fill cycles up to 199 cycles for each chamber The vacuum and pressure can be automatically regulated between 5 to 5 inches of water gauge in the main chamber and between 0 to 29 inches of mercury gauge in the transfer chamber See illustration below and figures in Chapter 3 Part Descriptions 5238600 Pressure Controller 115V or 100V 5...

Страница 44: ...g Kit Includes the FEP tubing and connections required for connecting typical drying train components such as moisture oxygen and other gases Fluorinated ethylene propylene FEP tubing has very low moisture absorption Part Descriptions 5242500 Drying Train Tubing Kit moisture solvents and radioisotopes 5242501 Drying Train Tubing Kit oxygen and moisture only See Figures 3 9 and 3 10 16 Gas and Vacu...

Страница 45: ...as a set of two 20 Acrylic Viewing Window with Frame Part 5236100 Acrylic window 1 2 thick for those users that prefer acrylic over the standard laminated safety glass includes metal frame 21 Guardian Jr Airflow Monitor Part 5242000 Audio and visual airflow monitor is especially useful for installations that exhaust the pressure relief valve to the outside Provides feedback on the roof mounted blo...

Страница 46: ... and fittings for installation Installing the pressure gauge requires drilling a 0 562 14 3mm diameter hole in the glove box ceiling 27 Vacuum Gauge for the Transfer Chamber Part 5237900 Kit for the transfer chamber is already installed on Precise Controlled Atmosphere Glove Boxes It can be added to the Precise Basic Glove Boxes Range 0 to 29 inches of mercury vacuum Kit includes the vacuum gauge ...

Страница 47: ...o electrical circuits PROBLEM CAUSE CORRECTIVE ACTION Lights won t operate Unit not plugged into outlet Plug unit into appropriate electrical service Circuit breaker s tripped Reset or replace circuit breaker Lamp not installed properly Inspect lamp installation Lamp wiring disconnected Inspect lamp wiring Defective lamp Replace lamp Light switch is defective Replace light switch Defective electro...

Страница 48: ...sert 6C 1ea 1408300 Large Ferrule 6D 1ea 1408400 Small Ferrule 6E 1ea 1413602 Stainless Compression Nut 6F 1ea 1413500 Hose Connector Secondary Method 6G order by the foot 1539500 Polyvinyl Chloride Tubing 3 8 OD x 3 16 ID 6H order by the foot 25 coils 7396209 FEP Tubing Drying Train 3 8 OD x 1 4 ID 6I 1ea 1409901 Tubing Insert Drying Train 3 8 OD x 1 4 ID 7 1ea 1365200 Main Glove Box Pressure Gau...

Страница 49: ...Appendix A Replacement Parts Product Service 1 800 522 7658 45 Figure A 1 Replacement Parts for Precise Glove Boxes Precise Controlled Atmosphere Glove Box Shown ...

Страница 50: ...B D Di im me en ns si io on ns s See the following dimensions for all the glove boxes Precise Controlled Atmosphere shown All dimensions in inches Figure B 1 Note Six valves pressure relief valve and two pressure gauges are omitted on the Precise Basic Glove Box ...

Страница 51: ...to 49 C Maximum relative humidity 80 for temperatures up to 88 F 31 C decreasing linearly to 50 relative humidity at 104 F 40 C Main supply voltage fluctuations not to exceed 10 of the nominal voltage Transient over voltages according to Installation Categories II Over voltage Categories per IEC 1010 Temporary voltage spikes on the AC input line that may be as high as 1500V for 115V models and 250...

Страница 52: ...ygen Level Per Number of Purge and Fill Cycles 54 3 2 45 4 4 40 5 2 35 6 2 30 7 4 25 8 8 20 10 5 15 12 6 10 15 1 5 18 1 1 20 3 125 0 6 105 0 8 88 1 2 78 1 5 70 1 9 0 5 10 15 20 25 0 20 40 60 80 100 120 140 of Purge and Fill Cycles 5 to 5 Inches of water pressure Oxygen Precise CA Glove Box Data Points Number of cycles Oxygen Results were attained at Labconco Corporation under controlled conditions...

Страница 53: ...8 0 7 70 1 3 54 2 8 42 5 35 6 2 30 7 5 25 9 1 20 11 15 13 2 10 15 8 5 19 1 22 1 0 5 10 15 20 25 0 10 20 30 40 50 60 70 80 90 of Purge and Fill Cycles 5 to 5 Inches of water pressure Moisture Precise CA Glove Box Data Points Number of cycles Water Results were attained at Labconco Corporation under controlled conditions Actual results may vary ...

Страница 54: ...Main Chamber Pressure Moisture Leak Rate Initial Moisture Moisture Column Life Life Capacity Weight Capacity of Columns inches of water ppm min ppm 24 hour days ppm grams of moisture 1 2 9 10000 3318 13864241 100 1 0 1 7 10000 5659 13864241 100 1 1 1 6 10000 6013 13864241 100 1 Note 1ppm of moisture 7 21ug of moisture inside the 368 liter glove box volume Note All data with glove port covers insta...

Страница 55: ...essure 0 50 100 150 200 250 300 350 400 0 10 20 30 40 50 60 70 80 Time Minutes ppm Moisture Oxygen Moisture 1 2 9 ppm min Moisture 0 1 7 ppm min Moisture 1 1 6 ppm min Oxygen 1 0 52ppm min Oxygen 0 0 22 ppm min Oxygen 1 0 15 ppm min Results were attained at Labconco Corporation under controlled conditions Actual results may vary ...

Страница 56: ...r http www descoemit com ViewProduct aspx pid 50661 h 1323 was installed in the glove box The DESCO ionizer is a dual steady state DC auto balancing bench top ionizer The ionizer helps to neutralize static electricity on surrounding surfaces The time required by the ionizer to neutralize static electricity will be the shortest when objects are within 12 to 36 directly in front of the ionizer The t...

Страница 57: ...he balance adjustment for the ionizer can be accomplished by inserting a small screwdriver or trimmer adjustment tool into the balance adjustment hole located on the side of the unit To increase the output in a positive direction turn the potentiometer in a clockwise direction Conversely to increase the output in a negative direction turn the potentiometer in a counter clockwise direction It is re...

Страница 58: ...st valve and allow box to pressurize to 5 H2O gauge than close valve Then with the vacuum pump running open the test outlet valve and allow the box to be evacuated to 5 H2O gauge then close valve Repeat this purge fill for a total of three cycles then close both valves 3 While Glove Box pressure is held at 5 0 minimum sniff all Glove Box joints seals and seams of the Glove Box shell using a test p...

Страница 59: ...6 to 10 w g Record data Check that valve reseals within 2 w g of opening pressure and opening vacuum Pressure relief valve is standard on Precise Controlled Atmosphere Glove Boxes Other Equipment 1 For a less critical helium leak measurement using a lower cost leak detector Labconco recommends using Restek www restek com or other instrumentation available from a laboratory supply dealer ...

Страница 60: ...Product Service 1 800 522 7658 56 A Ap pp pe en nd di ix x H H P Pl lu um mb bi in ng g D Di ia ag gr ra am ms s ...

Страница 61: ...Appendix F Ionizer Fan Performance Criteria Product Service 1 800 522 7658 57 ...

Страница 62: ... 760 29 92 406 8 33 90 1 14 70 1 033 101 3 1 013 lb in 2 51 71 2 036 27 69 2 307 06805 1 07031 6 895 0689 Kg cm2 735 6 28 96 393 7 32 81 9678 14 22 1 98 05 981 kPa 7 500 2953 4 016 3347 00987 1451 0102 1 01 bar 750 29 53 401 6 33 47 987 14 51 1 02 100 1 Flow Rate Conversions To From lit sec gal min ft3 sec ft3 min bbl hr bbl day lit sec 1 15 85 0 03532 2 119 22 66 543 8 gal min 0 06309 1 0 00223 0...

Страница 63: ...ntrol Pharmaceutical Press 2004 Editors Midcalf Phillips Neiger and Coles Isolation Technology A Practical Guide 2004 CRC Press 2nd Edition www crcpress com International Standard ISO 10648 2 Containment Enclosures Classification according to leak tightness and associated checking methods NIOSH Pocket Guide to Chemical Hazards U S Dept of Health and Human Resources Centers for Disease Control and ...

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